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[The occurrence and significance of plasma coagulase negative staphylococci from the genital tract of horses].

Abstract: Classification based on biochemical characteristics of 389 strains of plasma-coagulase-negative (plc-) staphylococci isolated from the genital tract of mares and stallions resulted in the following distribution of species: St. sciuri 130 (33.4%), St. equorum 42 (10.8%), St. xylosus 16 (4.1%), St. epidermidis 35 (9.0%), St. simulans 24 (6.2%), St. haemolyticus 33 (8.5%), St. warneri 18 (4.6%), St. lentus 12 (3.1%), St. hyicus 11 (2.8%). Strains of St. cohnii, St. capitis, St. gallinarum, St. saprophyticus and St. hominis have only been found sporadically (a. 1%). 48 (12.3%) strains could not be classified. With regard to species distribution of isolates from stallions and mares. 63.7% of the isolates from stallions belonged to St. sciuri and 9.3% to St. lentus, whereas in isolates of mares these species numbered only 24.9% and 0.4%, respectively. On the other hand the species St. equorum (14.9% vs. 6.8%), St. epidermidis (14.5% vs. 1.7%), St. haemolyticus (14.0% vs. 1.7%), St. warneri (7.7% vs. 0.8%) and St. xylosus (5.9% vs. 2.5%) predominated in mares. St. simulans was found occurring equally in mares and stallions (7.7% vs. 5.9%). Comparing the staphylococcal species of healthy mares and of mares which have not become pregnant after copulation no indication was found for a significant role of certain plc- staphylococci in infertility. All of the 389 isolates were tested for production of protein A, i.e. Fc-fragment binding receptors, using a microenzyme-assay with peroxidase-labelled rabbit immunoglobulin G. With this method cell-bound or extracellular Fc-receptors could not be detected in anyone of the plc- staphylococcal strains.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1989-05-01 PubMed ID: 2752930
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Summary

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This study explored the various plasma-coagulase negative (plc-) staphylococci types present in the genital tracts of mares and stallions, examined the variations in distribution between the two, and investigated the potential correlation between certain plc- staphylococci and infertility in mares. The testing for Fc-fragment binding receptors found no detectable receptors in any of the isolated plc- staphylococcal strains.

Staphylococci Distribution

  • The researchers isolated 389 strains of plc- staphylococci from horse genital tracts. These were identified and grouped based on biochemical properties into species including St. sciuri, St. equorum, St. xylosus, St. epidermidis, St. simulans, and many more.
  • There were notable variations in the representation of different bacterial species between mares and stallions. St. sciuri and St. Lentus were found to be more prevalent in stallions while St. equorum, St. epidermidis, St. haemolyticus, and others were more common in mares.
  • St. simulans was discovered at similar levels in both mares and stallions.

Investigation into Infertility

  • The study also looked at associations between the presence of specific staphylococcal species and mare infertility. However, no significant connection was discovered between any specific plc- staphylococci and mares not becoming pregnant following coitus.

Protein A Production Testing

  • All 389 plc- staphylococci strains were tested for protein A production, specifically for the production of Fc-fragment binding receptors. This involved a microenzyme-assay using peroxidase-labelled rabbit immunoglobulin G.
  • The test results revealed that none of the plc- staphylococcal strains had identifiable cell-bound or extracellular Fc-receptors, suggesting that this bacterial group does not produce Fc-fragment binding receptors.

Cite This Article

APA
Hüthwohl H, Weiss R, Schmeer N, Schliesser T. (1989). [The occurrence and significance of plasma coagulase negative staphylococci from the genital tract of horses]. Dtsch Tierarztl Wochenschr, 96(5), 256-258.

Publication

ISSN: 0341-6593
NlmUniqueID: 7706565
Country: Germany
Language: ger
Volume: 96
Issue: 5
Pages: 256-258

Researcher Affiliations

Hüthwohl, H
    Weiss, R
      Schmeer, N
        Schliesser, T

          MeSH Terms

          • Animals
          • Carrier State / microbiology
          • Carrier State / veterinary
          • Female
          • Genitalia / microbiology
          • Horse Diseases / microbiology
          • Horses
          • Male
          • Staphylococcal Infections / microbiology
          • Staphylococcal Infections / veterinary
          • Staphylococcus / isolation & purification

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