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The pathogenicity of Ab4p, the sequenced strain of equine herpesvirus-1, in specific pathogen-free foals.
Abstract: The sequencing of the genome of equine herpesvirus-1 (EHV-1) is reported in Elizabeth A. R. Telford, Moira S. Watson, Kathryn McBride, and Andrew J. Davison, 1992, Virology, 189, 304-316. The sequence was derived using a plaque-purified clone of EHV-1 strain Ab4 (designated Ab4p). To ensure that Ab4p shares the pathogenic characteristics of parental Ab4 (hereafter Ab4), both were inoculated intranasally into foals, specifically free from EHV-1 and EHV-4. Clinical signs, including rectal temperature, were similar for both viruses. In addition, nasal shedding of virus was observed over a 1- to 2-week period postinfection, and viremia was established with both Ab4 and Ab4p. Isolation of virus from one foal following intravenous administration of steroids indicates that Ab4p can establish latency and undergo reactivation. Finally, retinal lesions were observed and these were similar to those seen with Ab4. In conclusion, several pathogenic characteristics of Ab4 are retained in the plaque-purified clone, Ab4p.
Publication Date: 1992-07-01 PubMed ID: 1318607DOI: 10.1016/0042-6822(92)90707-vGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study investigated the pathogenic properties of the cloned strain of equine herpesvirus-1 (EHV-1), called Ab4p, in specific pathogen-free foals and found that it preserved many characteristics of its parental strain, Ab4.
Research Background
- The researchers set out to study the behavior of a sequenced strain of equine herpesvirus-1 (EHV-1), named Ab4p, in specific pathogen-free foals. The test strain was cloned from a parental strain called Ab4.
- The study aimed to understand if the pathogenic, or disease-causing, features of the parental Ab4 strain were retained in the cloned strain, Ab4p.
Methodology
- To conduct the study, both Ab4 and Ab4p viruses were introduced intranasally, or through the nose, into foals specifically chosen to be free from EHV-1 and another related virus, EHV-4.
- The researchers monitored the foals for clinical signs such as rectal temperature, indicating the presence and effects of the viruses.
- They also checked for traces of the virus in the foals’ nasal secretions over one to two weeks after infection, in a process known as nasal shedding. Establishing viremia, or the presence of the virus in the blood, was another process of monitoring.
- One foal was given steroids intravenously, and the researchers isolated the virus from this animal, indicating that the Ab4p strain could also establish latency, or a dormant state, and reactivate.
Findings
- The clinical signs, including rectal temperature and viremia, were found to be similar for both viruses.
- Nasal shedding of the virus was observed post-infection for both strains.
- The Ab4p strain was confirmed to be able to establish latency and have the potential for reactivation.
- Equally important, retinal lesions similar to those caused by the parental strain Ab4 were also seen in the case of the cloned Ab4p strain.
Conclusion
- The research concluded that the clone of the EHV-1 virus, Ab4p, retained several pathogenic characteristics of its parental strain, Ab4.
Cite This Article
APA
Gibson JS, Slater JD, Field HJ.
(1992).
The pathogenicity of Ab4p, the sequenced strain of equine herpesvirus-1, in specific pathogen-free foals.
Virology, 189(1), 317-319.
https://doi.org/10.1016/0042-6822(92)90707-v Publication
Researcher Affiliations
- Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom.
MeSH Terms
- Animals
- Antibodies, Viral / biosynthesis
- Antibody Formation
- Body Temperature
- Fever
- Herpesviridae Infections / pathology
- Herpesvirus 1, Equid / pathogenicity
- Horse Diseases / microbiology
- Horses
- Specific Pathogen-Free Organisms
Citations
This article has been cited 11 times.- Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
- Bryant NA, Wilkie GS, Russell CA, Compston L, Grafham D, Clissold L, McLay K, Medcalf L, Newton R, Davison AJ, Elton DM. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks. Transbound Emerg Dis 2018 Jun;65(3):817-832.
- Shakya AK, O'Callaghan DJ, Kim SK. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11. Front Vet Sci 2017;4:211.
- Okada A, Izume S, Ohya K, Fukushi H. Equine herpesvirus type 1 tegument protein VP22 is not essential for pathogenicity in a hamster model, but is required for efficient viral growth in cultured cells. J Vet Med Sci 2015 Oct;77(10):1293-7.
- Hussey GS, Goehring LS, Lunn DP, Hussey SB, Huang T, Osterrieder N, Powell C, Hand J, Holz C, Slater J. Experimental infection with equine herpesvirus type 1 (EHV-1) induces chorioretinal lesions. Vet Res 2013 Dec 5;44(1):118.
- Awan AR, Field HJ. Effects of phosphonylmethoxyalkyl derivatives studied with a murine model for abortion induced by equine herpesvirus 1. Antimicrob Agents Chemother 1993 Nov;37(11):2478-82.
- Kasem S, Abdel-Moneim AS, Fukushi H. Effect of thymidine kinase-deficiency (∆ORF38) on neuropathogenicity of equine herpesvirus-1 in the mouse model and expression of neighboring genes. Virus Genes 2025 Apr;61(2):179-192.
- Nayel M, Kasem S, Fukushi N, El-Habashi N, Elsify A, Salama A, Hassan H, Yanai T, Ohya K, Fukushi H. Equine Herpesvirus Type 1 ORF76 Encoding US9 as a Neurovirulence Factor in the Mouse Infection Model. Pathogens 2024 Oct 2;13(10).
- Nishimura F, Fukushi N, Sakai H, Fukushi H. Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30. J Vet Med Sci 2024 Dec 1;86(12):1273-1278.
- Giessler KS, Goehring LS, Jacob SI, Davis A, Esser MM, Lee Y, Zarski LM, Weber PSD, Hussey GS. Impact of the host immune response on the development of equine herpesvirus myeloencephalopathy in horses. J Gen Virol 2024 May;105(5).
- Pusterla N, Dorman DC, Burgess BA, Goehring L, Gross M, Osterrieder K, Soboll Hussey G, Lunn DP. Viremia and nasal shedding for the diagnosis of equine herpesvirus-1 infection in domesticated horses. J Vet Intern Med 2024 May-Jun;38(3):1765-1791.
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