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Scientific reports2021; 11(1); 4332; doi: 10.1038/s41598-021-83783-z

The pelvic flexure separates distinct microbial communities in the equine hindgut.

Abstract: As hindgut fermenters, horses are especially dependent on the microbiota residing in their cecum and large intestines. Interactions between these microbial populations and the horse are critical for maintaining gut homeostasis, which supports proper digestion. The current project was motivated to determine if any features of the fecal microbiota are informative of the microbial communities from the cecum, ventral colon, or dorsal colon. Digesta from the cecum, ventral colon, dorsal colon and feces were collected from 6 yearling miniature horses. Microbial DNA was isolated and the microbiota from each sample was characterized by profiling the V4 region of the 16S rRNA. Principal coordinate analysis of the beta diversity results revealed significant (p = 0.0001; F = 5.2393) similarities between the microbial populations from cecal and ventral colon and the dorsal colon and fecal samples, however, there was little overlap between the proximal and distal ends of the hindgut. These distinct population structures observed in our results coincide with the pelvic flexure, which itself separates intestinal compartments with distinct roles in digestive physiology. An indicator species analysis confirmed the population differences, supported by the identification of several microbial families characteristic of the compartments upstream of the pelvic flexure that were not represented following it. Our data suggest that the fecal microbiota is not informative of the proximal hindgut but can provide insight into communities of the distal compartments. Further, our results suggest that the pelvic flexure might be an important anatomical landmark relative to the microbial communities in the equine large intestine.
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Publication Date: 2021-02-22 PubMed ID: 33619300PubMed Central: PMC7900177DOI: 10.1038/s41598-021-83783-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the presence of distinct microbial communities in different parts of a horse’s gut, focusing specifically on the role of the pelvic flexure in separating these communities.

Research Motivation and Methodology

The research was motivated by the critical role microbiota play in the digestion process in horses, particularly in their cecum and large intestines. The team wanted to understand if any signs of the fecal microbiota could give information about the microbial communities in these segments of the horse’s hindgut.

Data was gathered by collecting samples from diverse regions of the gut, including the cecum, ventral colon, dorsal colon, and feces, from 6 yearling miniature horses. A molecular biology technique was employed, isolating microbial DNA and profiling the V4 region of the 16S rRNA to characterize the microbiota in each sample.

Findings

Analysing the beta diversity results, the team found significant similarities between the microbial populations in the cecal and ventral colon and between those in the dorsal colon and fecal samples. However, there was little overlap between the microbiota at the proximal (or earlier) and distal (or later) ends of the hindgut.

Interestingly, these different community populations align with the location of the pelvic flexure—an anatomical structure in the horse’s intestine. It separates the intestinal compartments, each of which has unique roles in digestive physiology.

Indicator Species Analysis and Conclusions

An indicator species analysis reaffirmed these population differences. Certain microbial families were found characteristic of the compartments situated upstream (or before) the pelvic flexure, with these families not evident in those located after it.

This led to two main conclusions:

  • The fecal microbiota cannot provide information about the microbiota in the proximal hindgut. However, it can offer insight into the microbial communities in the distal compartments.
  • The pelvic flexure appears to be a significant anatomical landmark related to the microbial communities in a horse’s large intestine.

This lays valuable groundwork for a more detailed understanding of the symbiotic relationship between horses and their internal microbial communities, which could have implications for the treatment of equine digestive diseases.

Cite This Article

APA
Reed KJ, Kunz IGZ, Scare JA, Nielsen MK, Turk PJ, Coleman RJ, Coleman SJ. (2021). The pelvic flexure separates distinct microbial communities in the equine hindgut. Sci Rep, 11(1), 4332. https://doi.org/10.1038/s41598-021-83783-z

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 11
Issue: 1
Pages: 4332
PII: 4332

Researcher Affiliations

Reed, Kailee J
  • Animal Sciences, Colorado State University, Fort Collins, CO, 80521, USA.
  • Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO, 80521, USA.
Kunz, Isabelle G Z
  • Animal Sciences, Colorado State University, Fort Collins, CO, 80521, USA.
Scare, Jessica A
  • M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40546, USA.
Nielsen, Martin K
  • M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40546, USA.
Turk, Philip J
  • Animal Sciences, Colorado State University, Fort Collins, CO, 80521, USA.
  • Atrium Health, Charlotte, NC, 28203, USA.
Coleman, Robert J
  • Animal and Food Sciences, University of Kentucky, Lexington, KY, 40546, USA.
Coleman, Stephen J
  • Animal Sciences, Colorado State University, Fort Collins, CO, 80521, USA. stephen.coleman@colostate.edu.
  • Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO, 80521, USA. stephen.coleman@colostate.edu.

MeSH Terms

  • Animals
  • Biodiversity
  • Gastrointestinal Microbiome
  • Horses
  • Intestine, Large
  • Metagenome
  • Metagenomics / methods
  • Pelvis / anatomy & histology

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 1 times.
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