The PGF2α agonists luprostiol and d-cloprostenol reliably induce luteolysis in luteal phase mares without evoking clinical side effects or a stress response.
Abstract: In the present study we have evaluated a possible stress reaction in response to two different PGF2α analogs-luprostiol and D-cloprostenol--and their effects on estrous cycle characteristics. In a cross-over-design eight mares received in alternating order either luprostiol (Treatment LUP; 3.75 mg im), D-cloprostenol (Treatment CLO; 22.5μg im) or saline (Treatment CON; NaCl 0.9% 0.5ml im) on day 8 after ovulation. Injection of either LUP or CLO, but not of CON resulted in a significant decline of progesterone concentration in plasma to baseline concentrations within two days (time: p<0.001, treatment: p<0.01, time × treatment: p<0.05). The treatment to ovulation interval was significantly shorter in LUP and CLO than in CON cycles (LUP: 9.4 ± 0.4 d; CLO: 9.4 ± 1.3 d; CON: 16.1 ± 0.8 d; p<0.001). Injection of either LUP or CLO, but not of CON significantly increased salivary cortisol concentration (immediately before injection: CON 1.3 ± 0.2, LUP 1.4 ± 0.3, CLO 1.4 ± 0.3 ng/ml; 60 min after injection: CON 1.0 ± 0.3, LUP 8.0 ± 1.4, CLO 4.2 ± 0.7 ng/ml; time: p<0.01, treatment: p<0.001, time × treatment: p<0.001). Heart rate decreased over time (p<0.05) independent of treatment and no changes in heart rate variability were detected. Injection of the PGF2α analogs CLO and LUP reliably induced luteolysis and apart from a transient increase in salivary cortisol concentration no signs of a physiological stress response or apparent side effects occurred.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication Date: 2016-03-02 PubMed ID: 26963045DOI: 10.1016/j.anireprosci.2016.02.031Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explored the stress reactions and effects on estrous cycle characteristics in response to the PGF2α analogs luprostiol and D-cloprostenol in mares. The study revealed that while both treatments caused a temporary increase in salivary cortisol, neither showed signs of a physiological stress response or apparent side effects.
Research Design and Methodology
- The study used a cross-over design and involved eight mares. The subjects were treated in alternating order with either luprostiol, D-cloprostenol, or saline (as a control) on the eighth day after ovulation.
- The objective was to establish the possible stress reaction caused by the two PGF2α analogs and how they impact the estrous cycle characteristics.
Key Findings
- Both the luprostiol and the D-cloprostenol treatments resulted in a marked decrease of progesterone concentration in the blood within two days, falling to baseline concentrations. The control (saline) showed no such decline.
- The interval between treatment and ovulation was significantly shorter in the case of both luprostiol and D-cloprostenol treatments when compared to the control treatment.
- Both of the PGF2α analog-treated groups showed a rise in salivary cortisol concentration, indicating a possible stress response.
- However, no other signs of a physiological stress response or evident side effects were observed after the administration of the PGF2α analogs.
Conclusions and Implications
- The study concluded that the PGF2α analogs, luprostiol and D-cloprostenol, can reliably induce luteolysis without any clinical side effects.
- Despite the temporary increase in salivary cortisol concentration, there were no evident signs of stress response or adverse effects.
- The research offers an avenue for further studying and understanding the hormonal regulation of the estrous cycle in mares and possible manipulation for fertility enhancements.
Cite This Article
APA
Kuhl J, Nagel C, Ille N, Aurich JE, Aurich C.
(2016).
The PGF2α agonists luprostiol and d-cloprostenol reliably induce luteolysis in luteal phase mares without evoking clinical side effects or a stress response.
Anim Reprod Sci, 168, 92-99.
https://doi.org/10.1016/j.anireprosci.2016.02.031 Publication
Researcher Affiliations
- Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Vienna, Austria. Electronic address: juliane.kuhl@vetmeduni.ac.at.
- Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Vienna, Austria.
- Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Vienna, Austria.
- Obstetrics, Gynecology and Andrology, Vetmeduni Vienna, Vienna, Austria.
- Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Vienna, Austria.
MeSH Terms
- Animals
- Behavior, Animal / drug effects
- Cloprostenol / adverse effects
- Cloprostenol / pharmacology
- Dinoprost / agonists
- Female
- Heart Rate / drug effects
- Horses / physiology
- Luteal Phase / drug effects
- Luteal Phase / physiology
- Luteolysis / drug effects
- Prostaglandins F, Synthetic / adverse effects
- Prostaglandins F, Synthetic / pharmacology
- Skin Temperature / drug effects
- Stress, Physiological / drug effects
Citations
This article has been cited 3 times.- Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares. Animals (Basel) 2021 May 28;11(6).
- Okada CTC, Kaps M, Perez Quesada J, Gautier C, Aurich J, Aurich C. Diestrous Ovulations in Pregnant Mares as a Response to Low Early Postovulatory Progestogen Concentration. Animals (Basel) 2020 Nov 30;10(12).
- Nagel C, Melchert M, Aurich C, Aurich J. Differences in Endocrine and Cardiac Changes in Mares and Her Fetus before, during, and after Parturition in Horses of Different Size. Animals (Basel) 2020 Sep 4;10(9).
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