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Home / Equine Research Bank / Vet Res Commun / The potential of three whole blood microRNAs to predict outc...
Veterinary research communications2022; 47(1); 87-98; doi: 10.1007/s11259-022-09930-7

The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids.

Abstract: MicroRNAs (miRNAs) have been proposed as biomarkers for equine sarcoid (ES) disease. In this study, the suitability of three whole blood miRNAs to diagnose ES and to predict and monitor the outcome of therapy was explored. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), expression levels of eca-miR-127, eca-miR-379, and eca-miR-432 in whole blood of ES-affected equids before and at least one year after therapy were compared to those of unaffected control equids. Associations of age, sex, species, diagnosis, and therapy outcome with miRNA expression levels were examined using general linear models. In total, 48 ES-affected equids and 47 control equids were recruited. From the affected animals, 31 responded favorably to treatment, and 17 demonstrated a failure of therapy. None of the tested miRNAs were influenced by age. Male equids showed increased expression of eca-miR-127 compared to females and horses showed higher expression levels of eca-miR-379 and eca-miR-432 than donkeys. Eca-miR-127 was confirmed as a diagnostic discriminator between ES-affected and control equids. No difference in miRNA profiles before therapy was found when comparing ES-affected equids with success vs. failure of therapy. Eca-miR-379 and eca-miR-432 decreased over time in horses where therapy was successful, but not in those cases where it failed. Biological variables influence equine whole blood miRNA expression, which may complicate biomarker validation. While none of the tested miRNAs could predict the response to therapy in ES-affected equids and eca-miR-127 showed poor diagnostic accuracy for ES, eca-miR-379 and eca-miR-432 miRNAs might allow refinement of monitoring of success of ES therapy.
© 2022. The Author(s).
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Publication Date: 2022-04-28 PubMed ID: 35484337PubMed Central: PMC9873782DOI: 10.1007/s11259-022-09930-7Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on the potential use of certain blood microRNAs as a tool to diagnose equine sarcoid (ES), a common type of skin tumor in horses, and to monitor the response to its treatment. The study found that two microRNAs might allow for better monitoring of the success of ES treatment.

Background and Objective

  • The research seeks to explore the potential use of three specific microRNAs (tiny molecules that play a key role in regulating gene expression) in the diagnosis and treatment of equine sarcoid (ES), a common skin tumor found in horses.
  • It aimed to assess whether levels of these microRNAs in the blood can serve as non-invasive biomarkers for the presence of ES and predict or monitor the response to treatments.

Methodology

  • Samples were collected from a total of 48 ES-affected equids (horses and donkeys), along with 47 unaffected control equids.
  • The researchers used a common laboratory technique, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), to measure the expression levels of three microRNAs (eca-miR-127, eca-miR-379, and eca-miR-432) in whole blood.
  • These levels were compared before and at least one year after the therapy. Various factors like age, sex, species, diagnosis, and therapy outcome were considered.

Findings

  • The study found that none of the microRNAs tested were influenced by age. It did find, however, that male equids had increased expression of eca-miR-127 compared to females. Similarly, horses showed higher expression levels of eca-miR-379 and eca-miR-432 than donkeys.
  • One of the microRNAs, eca-miR-127, was found to be a discerning factor between ES-affected and unaffected control equids, providing some potential for diagnostic use.
  • Interestingly, the study did not find any difference in the microRNA profiles before therapy when comparing ES-affected equids that responded to treatment with those where the treatment failed.
  • On the other hand, two microRNAs, eca-miR-379 and eca-miR-432, were observed to decrease over time in horses where therapy was successful, but not in those where it failed.

Implications

  • The research suggests that biological variables can influence the expression of microRNAs in equine whole blood, which may complicate their use as biomarkers.
  • While the study found that none of the tested microRNAs could predict response to therapy reliably, it suggests that monitoring the levels of eca-miR-379 and eca-miR-432 might help track the success of the treatment for sarcoid equids.

Cite This Article

APA
Hamza E, Cosandey J, Gerber V, Koch C, Unger L. (2022). The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids. Vet Res Commun, 47(1), 87-98. https://doi.org/10.1007/s11259-022-09930-7

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 47
Issue: 1
Pages: 87-98

Researcher Affiliations

Hamza, E
  • Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Departement of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Cosandey, J
  • Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Gerber, V
  • Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Koch, C
  • Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Unger, L
  • Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland. lucia.unger@vetsuisse.unibe.ch.

MeSH Terms

  • Female
  • Male
  • Horses
  • Animals
  • MicroRNAs / genetics
  • Biomarkers
  • Skin Diseases / veterinary
  • Horse Diseases / diagnosis
  • Horse Diseases / genetics

Conflict of Interest Statement

The authors have no relevant financial or non-financial interests to disclose.

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