The presence and gene expression of bovine papillomavirus in the peripheral blood and semen of healthy horses.
Abstract: Papillomavirus (PV) are double-stranded DNA viruses that can cause both benignant and malignant tumours in mammals. Twelve genotypes of bovine papillomavirus (BPV1-12) have been identified so far. The presence of BPV1 and 2 has been found in the body fluids of cattle and horses. The aim of this study is to investigate the presence of BPV DNA and the expression of viral genes in the blood and sperm cells of healthy horses using PCR and RT-PCR. BPV-1 or 2 was detected in 14 of 70 blood samples (20%) and in 11 of 31 semen samples (35%). In five of fourteen blood samples, the E5 expression tested positive, while no blood sample was positive for L1 expression. Four of 11 (36%) semen cell samples proved to be positive for E5 expression, while no gene expression in L1 could be detected. This is the first study that shows BPV1 gene expression in the blood and semen of healthy horses. Our data illustrate the need for a better understanding of the presence of BPV in non-epithelial tissues of horses and their role in the vertical and horizontal transmission of these viruses.
© 2012 Blackwell Verlag GmbH.
Publication Date: 2012-12-04 PubMed ID: 23210736DOI: 10.1111/tbed.12036Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The paper explores the presence and activity of bovine papillomavirus (BPV) in healthy horses’ blood and semen. The research shows the first evidence of BPV1 gene expression in these samples, suggesting a need for more understanding of how BPV behaves in non-epithelial tissues of horses.
Introduction to Papillomavirus and its Relevance
- Papillomaviruses (PV) are DNA viruses that can lead to both benignant and malignant tumors in mammals.
- So far, twelve different genotypes of bovine papillomavirus (BPV1-12) have been identified.
- BPV1 and 2 have been found in body fluids of cattle and horses, establishing their presence in these animals.
Objective of the Study
- The pivotal aim of this study is to analyze the presence of BPV DNA and the active expression of their genes in the blood and sperm cells of healthy horses.
- The research implements PCR and RT-PCR methods in the investigation.
Results of the Study
- In blood samples, BPV-1 or 2 was detected in 20% of cases, and 35% of semen samples showcased the same.
- The E5 expression was observable in a percentage of these samples, but none of them showed L1 expression.
Significance of the Study
- This study is groundbreaking as it provides the first evidence of BPV1 gene expressions in the blood and semen of healthy horses.
- The study’s findings support the need for a more profound understanding of how BPV is present in horse tissues that are not epithelial and its implications on vertical and horizontal transmission of these viruses.
Cite This Article
APA
Silva MA, Silva KM, Jesus AL, Barros LO, Corteggio A, Altamura G, Borzacchiello G, Freitas AC.
(2012).
The presence and gene expression of bovine papillomavirus in the peripheral blood and semen of healthy horses.
Transbound Emerg Dis, 61(4), 329-333.
https://doi.org/10.1111/tbed.12036 Publication
Researcher Affiliations
- Department of Genetics, Center for Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.
MeSH Terms
- Animals
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / isolation & purification
- DNA, Viral / blood
- DNA, Viral / genetics
- Gene Expression Regulation, Viral / physiology
- Horses / blood
- Horses / virology
- Male
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
- RNA, Viral / genetics
- RNA, Viral / metabolism
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Semen / virology
Citations
This article has been cited 7 times.- Maggi R, De Paolis L, De Santis D, Vellone VG, De Ciucis CG, Fruscione F, Mazzocco K, Ghelardi A, Marruchella G, Razzuoli E. Bovine Papillomavirus Type 1 Infection in an Equine Congenital Papilloma. Pathogens 2023 Aug 18;12(8).
- Savini F, Gallina L, Mazza F, Mariella J, Castagnetti C, Scagliarini A. Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals. Vet Sci 2019 Feb 6;6(1).
- Silva MA, Silva EC, Gurgel AP, Nascimento KC, Freitas AC. Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls. Virusdisease 2014 Jan;25(1):125-8.
- DiMaio D, Petti LM. The E5 proteins. Virology 2013 Oct;445(1-2):99-114.
- Cutarelli A, De Falco F, Serpe F, Izzo S, Fusco G, Catoi C, Roperto S. Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses. Sci Rep 2025 Jan 4;15(1):769.
- Cutarelli A, De Falco F, Brunetti R, Napoletano M, Fusco G, Roperto S. Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen. Front Vet Sci 2024;11:1427370.
- Pontes NE, Carrazzoni PG, Pessoa-Junior ME, Tibúrcio Júnior E, Freitas AC, Silva MARD. Bovine papillomavirus vertical transmission: BPV diversity and expression in maternal and fetal tissues. Braz J Microbiol 2024 Sep;55(3):2879-2884.
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