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Home / Equine Research Bank / Microorganisms / The Presence of Treponema spp. in Equine Hoof Canker Biopsie...
Microorganisms2021; 9(11); doi: 10.3390/microorganisms9112190

The Presence of Treponema spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions.

Abstract: Equine hoof canker and bovine digital dermatitis are infectious inflammatory diseases of the hooves with an unknown etiology. However, anaerobic spirochetes of the genus Treponema are considered to be potential etiological agents. The aim of this study was to find a suitable way to isolate DNA and to detect the presence of treponemal DNA in samples of equine hoof canker and bovine digital dermatitis. DNAzol®® Direct and column kits were used to isolate DNA from samples of equine hoof canker and bovine digital dermatitis. The presence of Treponema spp. was detected using PCR and Sanger sequencing. DNAzol®® Direct is suitable for isolating DNA from these types of samples. Treponemal DNA was detected in equine hoof samples as well as in bovine digital dermatitis skin samples. In equine hoof biopsies, the most frequently detected was Treponema pedis (8/13). Treponema brennaborense (2/13) and Treponema denticola (2/13) were also found. In the case of bovine digital dermatitis, Treponema medium ssp. bovis was confirmed in 14 of 36 skin samples. Treponema pedis (9/36), Treponema vincentii (1/36), Treponema phagedenis (1/36), and Treponema brennaborense (1/36) were detected as well. DNAzol®® Direct was more appropriate for isolation of treponemal DNA because the columns isolation method was more equipment and time-consuming. The presence of several Treponema spp. was determined in the samples. In horses, the most commonly detected species was a T. pedis, while in cattle it was T. medium ssp. bovis.
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Publication Date: 2021-10-20 PubMed ID: 34835315PubMed Central: PMC8625648DOI: 10.3390/microorganisms9112190Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates possible causes of hoof diseases in horses and cattle by identifying Treponema bacteria species in affected tissue samples. Special DNA isolation techniques were used to reveal that different Treponema species were present, supporting the hypothesis that these bacteria could be involved in the diseases.

Research Methods and Objectives

  • The main aim of the study was to identify the presence of treponemal DNA in samples from equine hoof canker and bovine digital dermatitis to determine whether this bacteria genus could be a cause of these diseases. Both these conditions are infectious and inflammatory but their exact cause is unknown.
  • The researchers wanted to establish a reliable method of extracting treponemal DNA from such samples, hence they used both DNAzol Direct and column kit methods for isolation. The use of PCR (Polymerase Chain Reaction) and Sanger sequencing allowed them to specifically target and detect Treponema species organises.

Findings

  • This study demonstrated the presence of various Treponema species in both equine hoof and bovine digital dermatitis samples.
  • In the equine samples the most frequently detected was Treponema pedis, while in the bovine samples the most common species was Treponema phagedenis.
  • The DNAzol Direct method of DNA isolation proved the most effective, as the column kit involved was more time consuming and needed more equipment.

Implications and Conclusions

  • The research provides supporting evidence that anaerobic spirochetes of the Treponema genus could be involved in the etiology of these hoof diseases recognized in horses and cattle.
  • The methodology established for DNA extraction and detection could be useful in further research focused on such diseases and also in developing preventive measures or treatments.
  • The specific species identified in each animal type could be a starting point for detailed studies about the pathological role these bacteria play in each disease.

Cite This Article

APA
Marčeková P, Mad'ar M, Styková E, Kačírová J, Sondorová M, Mudroň P, Žert Z. (2021). The Presence of Treponema spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions. Microorganisms, 9(11). https://doi.org/10.3390/microorganisms9112190

Publication

Microorganisms
ISSN: 2076-2607
NlmUniqueID: 101625893
Country: Switzerland
Language: English
Volume: 9
Issue: 11

Researcher Affiliations

Marčeková, Paulína
  • Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Mad'ar, Marián
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Styková, Eva
  • Clinic of Horses, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Kačírová, Jana
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Sondorová, Miriam
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Mudroň, Pavol
  • Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Žert, Zdeněk
  • Clinic of Horses, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.

Grant Funding

  • APVV-16-0203 / Slovak Research and Development Agency
  • APVV-19-0462 / Slovak Research and Development Agency

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Navarrete-López P, Asselstine V, Maroto M, Lombó M, Cánovas Á, Gutiérrez-Adán A. RNA Sequencing of Sperm from Healthy Cattle and Horses Reveals the Presence of a Large Bacterial Population. Curr Issues Mol Biol 2024 Sep 19;46(9):10430-10443.
    doi: 10.3390/cimb46090620pubmed: 39329972google scholar: lookup
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