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Microorganisms2021; 9(11); doi: 10.3390/microorganisms9112190

The Presence of Treponema spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions.

Abstract: Equine hoof canker and bovine digital dermatitis are infectious inflammatory diseases of the hooves with an unknown etiology. However, anaerobic spirochetes of the genus Treponema are considered to be potential etiological agents. The aim of this study was to find a suitable way to isolate DNA and to detect the presence of treponemal DNA in samples of equine hoof canker and bovine digital dermatitis. DNAzol®® Direct and column kits were used to isolate DNA from samples of equine hoof canker and bovine digital dermatitis. The presence of Treponema spp. was detected using PCR and Sanger sequencing. DNAzol®® Direct is suitable for isolating DNA from these types of samples. Treponemal DNA was detected in equine hoof samples as well as in bovine digital dermatitis skin samples. In equine hoof biopsies, the most frequently detected was Treponema pedis (8/13). Treponema brennaborense (2/13) and Treponema denticola (2/13) were also found. In the case of bovine digital dermatitis, Treponema medium ssp. bovis was confirmed in 14 of 36 skin samples. Treponema pedis (9/36), Treponema vincentii (1/36), Treponema phagedenis (1/36), and Treponema brennaborense (1/36) were detected as well. DNAzol®® Direct was more appropriate for isolation of treponemal DNA because the columns isolation method was more equipment and time-consuming. The presence of several Treponema spp. was determined in the samples. In horses, the most commonly detected species was a T. pedis, while in cattle it was T. medium ssp. bovis.
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Publication Date: 2021-10-20 PubMed ID: 34835315PubMed Central: PMC8625648DOI: 10.3390/microorganisms9112190Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates possible causes of hoof diseases in horses and cattle by identifying Treponema bacteria species in affected tissue samples. Special DNA isolation techniques were used to reveal that different Treponema species were present, supporting the hypothesis that these bacteria could be involved in the diseases.

Research Methods and Objectives

  • The main aim of the study was to identify the presence of treponemal DNA in samples from equine hoof canker and bovine digital dermatitis to determine whether this bacteria genus could be a cause of these diseases. Both these conditions are infectious and inflammatory but their exact cause is unknown.
  • The researchers wanted to establish a reliable method of extracting treponemal DNA from such samples, hence they used both DNAzol Direct and column kit methods for isolation. The use of PCR (Polymerase Chain Reaction) and Sanger sequencing allowed them to specifically target and detect Treponema species organises.

Findings

  • This study demonstrated the presence of various Treponema species in both equine hoof and bovine digital dermatitis samples.
  • In the equine samples the most frequently detected was Treponema pedis, while in the bovine samples the most common species was Treponema phagedenis.
  • The DNAzol Direct method of DNA isolation proved the most effective, as the column kit involved was more time consuming and needed more equipment.

Implications and Conclusions

  • The research provides supporting evidence that anaerobic spirochetes of the Treponema genus could be involved in the etiology of these hoof diseases recognized in horses and cattle.
  • The methodology established for DNA extraction and detection could be useful in further research focused on such diseases and also in developing preventive measures or treatments.
  • The specific species identified in each animal type could be a starting point for detailed studies about the pathological role these bacteria play in each disease.

Cite This Article

APA
Marčeková P, Mad'ar M, Styková E, Kačírová J, Sondorová M, Mudroň P, Žert Z. (2021). The Presence of Treponema spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions. Microorganisms, 9(11). https://doi.org/10.3390/microorganisms9112190

Publication

Microorganisms
ISSN: 2076-2607
NlmUniqueID: 101625893
Country: Switzerland
Language: English
Volume: 9
Issue: 11

Researcher Affiliations

Marčeková, Paulína
  • Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Mad'ar, Marián
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Styková, Eva
  • Clinic of Horses, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Kačírová, Jana
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Sondorová, Miriam
  • Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Mudroň, Pavol
  • Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Žert, Zdeněk
  • Clinic of Horses, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.

Grant Funding

  • APVV-16-0203 / Slovak Research and Development Agency
  • APVV-19-0462 / Slovak Research and Development Agency

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 32 references
  1. Gillespie AV, Carter SD, Blowey RW, Staton GJ, Evans NJ. Removal of bovine digital dermatitis-associated treponemes from hoof knives after foot-trimming: A disinfection field study. BMC Vet. Res. 2020;16:330.
    doi: 10.1186/s12917-020-02552-8pmc: PMC7488572pubmed: 32917195google scholar: lookup
  2. Staton GJ, Sullivan LE, Blowey RW, Carter SD, Evans NJ. Surveying bovine digital dermatitis and non-healing bovine foot lesions for the presence of Fusobacterium necrophorum, Porphyromonas endodontalis and Treponema pallidum. Vet Rec. 2020;186:450.
    doi: 10.1136/vr.105628pmc: PMC7279135pubmed: 32066637google scholar: lookup
  3. Newbrook K, Carter SD, Crosby-Durrani H, Evans NJ. Challenge of Bovine Foot Skin Fibroblasts with Digital Dermatitis Treponemes Identifies Distinct Pathogenic Mechanisms. Front. Cell. Infect. Microbiol. 2021;10:538591.
    doi: 10.3389/fcimb.2020.538591pmc: PMC7820575pubmed: 33489929google scholar: lookup
  4. Hartshorn RE, Thomas EC, Anklam K, Lopez-Benavides MG, Buchalova M, Hemling TC, Döpfer D. Short communication: Minimum bactericidal concentration of disinfectants evaluated for bovine digital dermatitis-associated Treponema phagedenis-like spirochetes. J. Dairy Sci. 2013;96:3034–3038.
    doi: 10.3168/jds.2012-5994pubmed: 23498015google scholar: lookup
  5. Anklam K, Kulow M, Yamazaki W, Döpfer D. Development of real-time PCR and loop-mediated isothermal amplification (LAMP) assays for the differential detection of digital dermatitis associated treponemes. PLoS ONE 2017;12:e0178349.
    doi: 10.1371/journal.pone.0178349pmc: PMC5444799pubmed: 28542573google scholar: lookup
  6. Kuwano A, Niwa H, Higuchi T, Mitsui H, Agne RA. Treponemes-infected canker in a Japanese racehorse: Efficacy of maggot debridement therapy. J. Equine Sci. 2012;23:41–46.
    doi: 10.1294/jes.23.41pmc: PMC4013979pubmed: 24833994google scholar: lookup
  7. Apprich V, Licka T, Zipfl N, Tichy A, Gabriel C. Equine Hoof Canker: Cell Proliferation and Morphology. Vet. Pathol. 2017;54:661–668.
    doi: 10.1177/0300985817695515pubmed: 28622496google scholar: lookup
  8. Oosterlinck M, Deneut K, Dumoulin M, Gasthuys F, Pille F. Retrospective study on 30 horses with chronic proliferative pododermatitis (canker). Equine Vet. Educ. 2011;23:466–471.
    doi: 10.1111/j.2042-3292.2010.00213.xgoogle scholar: lookup
  9. Azzolini EFOT, Bastos SF, Barros RM. Chronic equine proliferative pododermatitis: Case report. Braz. J. Vet. Res. Anim. Sci. 2019;56:e160249.
    doi: 10.11606/issn.1678-4456.bjvras.2019.160249google scholar: lookup
  10. Fürst AE, Lische CJ. Chapter 90-Foot. In: Auer JA, Stick JA, editors. Equine Surgery. 4th ed. Elsevier Saunders; Philadelphia, PA, USA: 2012. pp. 1264–1299.
    doi: 10.1016/B978-1-4377-0867-7.00090-9google scholar: lookup
  11. Brandt S, Schoster A, Tober R, Kainzbauer C, Burgstaller JP, Haralambus R, Steinborn R, Hinterhofer C, Stanek C. Consistent detection of bovine papillomavirus in lesions, intact skin and peripheral blood mononuclear cells of horses affected by hoof canker. Equine Vet. J. 2011;43:202–209.
    doi: 10.1111/j.2042-3306.2010.00147.xpubmed: 21592216google scholar: lookup
  12. Sykora S, Brandt S. Occurrence of Treponema DNA in equine hoof canker and normal hoof tissue. Equine Vet. J. 2015;47:627–630.
    doi: 10.1111/evj.12327pubmed: 25115798google scholar: lookup
  13. Espiritu HM, Mamuad LL, Jin SJ, Kim SH, Kwon SW, Lee SS, Lee SM, Cho YI. Genotypic and Phenotypic Characterization of Treponema phagedenis from Bovine Digital Dermatitis. Microorganisms 2020;8:1520.
    doi: 10.3390/microorganisms8101520pmc: PMC7600072pubmed: 33023205google scholar: lookup
  14. Nises J, Rosander A, Pettersson A, Backhans A. The occurrence of Treponema spp. in gingival plaque from dogs with varying degree of periodontal disease. PLoS ONE 2018;13:e0201888.
    doi: 10.1371/journal.pone.0201888pmc: PMC6084996pubmed: 30092089google scholar: lookup
  15. Tanno-Nakanishi M, Kikuchi Y, Kokubu E, Yamada S, Ishihara K. Treponema denticola transcriptional profiles in serum-restricted conditions. FEMS Microbiol. Lett. 2018;365:fny171.
    doi: 10.1093/femsle/fny171pubmed: 29982599google scholar: lookup
  16. Mamuad LL, Seo BJ, Faruk MSA, Espiritu HM, Jin SJ, Kim WI, Lee SS, Cho YI. Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle. Vet. Microbiol. 2020;245:108696.
    doi: 10.1016/j.vetmic.2020.108696pubmed: 32456812google scholar: lookup
  17. Buyuktimkin B, Zafar H, Saier MH Jr. Comparative genomics of the transportome of Ten Treponema species. Microb. Pathog. 2019;132:87–99.
    doi: 10.1016/j.micpath.2019.04.034pmc: PMC7085940pubmed: 31029716google scholar: lookup
  18. Nagamine CM, Castro F, Buchanan B, Schumacher J, Craig JLE. Proliferative pododermatitis (canker) with intralesional spirochetes in three horses. Vet. Diagn. Investig. 2005;17:269–271.
    doi: 10.1177/104063870501700311pubmed: 15945386google scholar: lookup
  19. Moe KK, Yano T, Kuwano A, Sasaki S, Misawa N. Detection of treponemes in canker lesions of horses by 16S rRNA clonal sequencing analysis. J. Vet. Med. Sci. 2010;72:235–239.
    doi: 10.1292/jvms.09-0404pubmed: 19942809google scholar: lookup
  20. Brandt S, Apprich V, Hackl V, Tober R, Danzer M, Kainzbauer C, Gabriel C, Stanek C, Kofler J. Prevalence of bovine papillomavirus and Treponema DNA in bovine digital dermatitis lesions. Vet. Microbiol. 2011;148:161–167.
    doi: 10.1016/j.vetmic.2010.08.031pubmed: 20875931google scholar: lookup
  21. Klitgaard K, Nielsen MW, Ingerslev HC, Boye M, Jensen TK. Discovery of bovine digital dermatitis-associated Treponema spp. in the dairy herd environment by a targeted deep-sequencing approach. Appl. Environ. Microbiol. 2014;80:4427–4432.
    doi: 10.1128/AEM.00873-14pmc: PMC4068665pubmed: 24814794google scholar: lookup
  22. Kuhnert P, Brodard I, Alsaaod M, Steiner A, Stoffel MH, Jores J. Treponema phagedenis (ex Noguchi 1912) Brumpt 1922 sp. nov., nom. rev., isolated from bovine digital dermatitis. Int. J. Syst. Evol. Microbiol. 2020;70:2115–2123.
    doi: 10.1099/ijsem.0.004027pubmed: 31999237google scholar: lookup
  23. Bomjardim HA, Oliveira MC, Cordeiro MD, Brito MF, Fonseca AH, Oliveira C, Silva NS, Barbosa JD. Detection of Treponema spp. in bovine digital dermatitis in the Amazon biome, Brazil. Pesquisa Veterinária Brasileira 2020;40:430–437.
    doi: 10.1590/1678-5150-pvb-6139google scholar: lookup
  24. Nordhoff M, Moter A, Schrank K, Wieler LH. High prevalence of treponemes in bovine digital dermatitis-a molecular epidemiology. Vet. Microbiol. 2008;131:293–300.
    doi: 10.1016/j.vetmic.2008.04.019pubmed: 18524502google scholar: lookup
  25. Nascimento LV, Mauerwerk MT, Dos Santos CL, Barros Filho IR, BirgelJúnior EH, Sotomaior CS, Madeira HM, Ollhoff RD. Treponemes detected in digital dermatitis lesions in Brazilian dairy cattle and possible host reservoirs of infection. J. Clin. Microbiol. 2015;53:1935–1937.
    doi: 10.1128/JCM.03586-14pmc: PMC4432058pubmed: 25788552google scholar: lookup
  26. Daly K, Stewart CS, Flint HJ, Shirazi-Beechey SP. Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes. FEMS Microbiol. Ecol. 2001;38:141–151.
    doi: 10.1111/j.1574-6941.2001.tb00892.xgoogle scholar: lookup
  27. Steelman SM, Chowdhary BP, Dowd S, Suchodolski J, Janečka JE. Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis. BMC Vet. Res. 2012;8:231.
    doi: 10.1186/1746-6148-8-231pmc: PMC3538718pubmed: 23186268google scholar: lookup
  28. Demirkan I, Erdoğan M, Demirkan AÇ, Bozkurt F, Altındiş M, Navruz FZ, Köse Z. Isolation and identification of Treponema pedis and Treponema phagedenis-like organisms from bovine digital dermatitis lesions found in dairy cattle in Turkey. J. Dairy Sci. 2018;101:10317–10326.
    doi: 10.3168/jds.2017-14227pubmed: 30219415google scholar: lookup
  29. Wilson-Welder JH, Alt DP, Nally JE. Digital Dermatitis in Cattle: Current Bacterial and Immunological Findings. Animals 2015;5:1114–1135.
    doi: 10.3390/ani5040400pmc: PMC4693204pubmed: 26569318google scholar: lookup
  30. Moreira TF, Facury Filho EJ, Carvalho AU, Strube ML, Nielsen MW, Klitgaard K, Jensen TK. Pathology and bacteria related to digital dermatitis in dairy cattle in all year round grazing system in Brazil. PLoS ONE 2018;13:e0193870.
    doi: 10.1371/journal.pone.0193870pmc: PMC5841792pubmed: 29513739google scholar: lookup
  31. Alsaaod M, Locher I, Jores J, Grimm P, Brodard I, Steiner A, Kuhnert P. Detection of specific Treponema species and Dichelobacter nodosusfrom digital dermatitis (Mortellaro’s disease) lesions in Swiss cattle. Schweiz. Arch. Tierheilkd. 2019;161:207–215.
    doi: 10.17236/sat00201pubmed: 30942188google scholar: lookup
  32. Beninger C, Naqvi SA, Naushad S, Orsel K, Luby C, Derakhshani H, Khafipour E, De Buck J. Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species. Vet. Res. 2018;49:111.
    doi: 10.1186/s13567-018-0605-zpmc: PMC6206660pubmed: 30373670google scholar: lookup

Citations

This article has been cited 1 times.
  1. Navarrete-López P, Asselstine V, Maroto M, Lombó M, Cánovas Á, Gutiérrez-Adán A. RNA Sequencing of Sperm from Healthy Cattle and Horses Reveals the Presence of a Large Bacterial Population. Curr Issues Mol Biol 2024 Sep 19;46(9):10430-10443.
    doi: 10.3390/cimb46090620pubmed: 39329972google scholar: lookup
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