The relationship between Corynebacterium pseudotuberculosis biovar equi phenotype with location and extent of lesions in horses.
Abstract: Equine infection with Corynebacterium pseudotuberculosis can manifest in several forms, including external or internal abscesses. The objective of this study was to phenotype clinical isolates of C. pseudotuberculosis and to investigate the relationship between lesion location and extent of lesions in the animals from which they were collected. One hundred and seventy-one C. pseudotuberculosis biovar equi isolates were collected from horses presenting to the University of California Veterinary Medical Teaching Hospital and two other sources in the period between September 1996 and December 2011. Bacterial isolates were grouped on the bases of biochemical characteristics and growth on brain heart infusion agar. Six phenotypes were identified: (1) large colonies that metabolized sucrose (n = 81); (2) large sucrose-negative colonies (n = 47); (3) medium sucrose-positive (n = 20); (4) medium sucrose-negative (n = 11); (5) small sucrose-positive (n = 7), and (6) small sucrose-negative (n = 5). Medical records corresponding to each isolate were accessed from the University's administrative computer system or from the submitting source in order to determine the anatomical site from which the isolate was collected (n = 171), as well as the extent of lesions (n = 164) in the patient. The relationship between phenotype, lesion location and extent of lesions was then investigated statistically. No significant relationship between strain and lesion location or extent of lesions was found. This suggests that phenotypic differences during in vitro culture does not account for external versus internal disease in horses. Further work to characterize strains genotypically and to identify determinants for bacterial virulence should be performed. Importantly, host and environmental factors should also be further investigated.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication Date: 2014-04-02 PubMed ID: 24703322DOI: 10.1016/j.tvjl.2014.03.009Google Scholar: Lookup
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- Journal Article
Summary
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The research paper investigates the connection between distinctive categories (phenotypes) of a bacteria called Corynebacterium pseudotuberculosis and the location and severity of the lesions it causes in horses. Found no significant correlation, implying that internal and external diseases in horses might not be due to variations observed in laboratory culture.
Study Design and Data Collection
- The research used 171 isolates of Corynebacterium pseudotuberculosis biovar equi collected from horses presented to the University of California Veterinary Medical Teaching Hospital and two other sources over 15 years (1996–2011).
- The researchers separated and classified the bacterial isolates based on their biochemical characteristics and growth on brain heart infusion agar, a type of growth medium. They identified six different phenotypes based on size of the colony as well as its ability to metabolize sucrose.
- To match each bacterial isolate with the horse it originated from, the researchers used medical records from the University’s administrative computer system or from the submitting source, accessing information on where the isolate was collected in the body and the severity of the lesions found in the subject.
Findings and Conclusion
- The researchers examined the relationship between the type (phenotype) of Corynebacterium pseudotuberculosis, the site of the lesion in the horse, and the severity of the lesions.
- No significant relationship between the phenotype of the bacteria and the location or extent of the lesions was found. This indicates that the different disease manifestations (external or internal abscesses) seen in horses are not linked to the biochemical or growth characteristics of the strains as studied under laboratory conditions.
- The study recommends further research to understand the genetic variants of these bacterial isolates and to identify the precise factors leading to their virulence in order to manage and treat this disease more effectively in horses.
- The paper also emphasizes the importance of exploring host (such as immunological responses) and environmental factors that could contribute to the disease progression.
Cite This Article
APA
Britz E, Spier SJ, Kass PH, Edman JM, Foley JE.
(2014).
The relationship between Corynebacterium pseudotuberculosis biovar equi phenotype with location and extent of lesions in horses.
Vet J, 200(2), 282-286.
https://doi.org/10.1016/j.tvjl.2014.03.009 Publication
Researcher Affiliations
- PO Box 261069, Los Angeles, CA 20026, USA. Electronic address: eline.britz.dvm@gmail.com.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
- Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
MeSH Terms
- Actinomycetales Infections / microbiology
- Actinomycetales Infections / veterinary
- Animals
- California
- Horse Diseases / microbiology
- Horses
- Phenotype
- Random Amplified Polymorphic DNA Technique / veterinary
- Rhodococcus equi / classification
- Rhodococcus equi / genetics
- Rhodococcus equi / physiology
Citations
This article has been cited 8 times.- Marques da Silva W, Seyffert N, Silva A, Azevedo V. A journey through the Corynebacterium pseudotuberculosis proteome promotes insights into its functional genome. PeerJ 2021;9:e12456.
- Rifici C, Attili AR, De Biase D, Gonçalves Dos Santos R, Seyffert N, De Paula Castro TL, Pereira Figueiredo HC, Scaramozzino C, Reale S, Paciello O, Cuteri V, Spier SJ, Azevedo V, Mazzullo G. Atypical Multibacterial Granulomatous Myositis in a Horse: First Report in Italy. Vet Sci 2020 Apr 21;7(2).
- Silva WM, Carvalho RDO, Dorella FA, Folador EL, Souza GHMF, Pimenta AMC, Figueiredo HCP, Le Loir Y, Silva A, Azevedo V. Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host. Front Cell Infect Microbiol 2017;7:325.
- Silva WM, Folador EL, Soares SC, Souza GHMF, Santos AV, Sousa CS, Figueiredo H, Miyoshi A, Le Loir Y, Silva A, Azevedo V. Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains. BMC Genomics 2017 Jun 8;18(1):451.
- Baraúna RA, Ramos RT, Veras AA, Pinheiro KC, Benevides LJ, Viana MV, Guimarães LC, Edman JM, Spier SJ, Azevedo V, Silva A. Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics. PLoS One 2017;12(1):e0170676.
- Oliveira A, Teixeira P, Azevedo M, Jamal SB, Tiwari S, Almeida S, Silva A, Barh D, Dorneles EM, Haas DJ, Heinemann MB, Ghosh P, Lage AP, Figueiredo H, Ferreira RS, Azevedo V. Corynebacterium pseudotuberculosis may be under anagenesis and biovar Equi forms biovar Ovis: a phylogenic inference from sequence and structural analysis. BMC Microbiol 2016 Jun 2;16:100.
- Barba M, Stewart AJ, Passler T, Wooldridge AA, van Santen E, Chamorro MF, Cattley RC, Hathcock T, Hogsette JA, Hu XP. Experimental transmission of Corynebacterium pseudotuberculosis biovar equi in horses by house flies. J Vet Intern Med 2015 Mar-Apr;29(2):636-43.
- Markova J, Langova D, Babak V, Kostovova I. Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms-A Comparative Study. Microorganisms 2024 Apr 27;12(5).
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