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The separation of peripheral blood cells of the horse.

Abstract: The peripheral blood cells from Standard bred horses were subjected to procedures which will separate equine peripheral blood cells with good precision and efficiency into red cell, leukocyte, and platelet fractions. The separated cells have normal morphology and the differential count of the separated granulocytes and lymphocytes is unchanged from that of the original sample.
Publication Date: 1974-01-01 PubMed ID: 4272960PubMed Central: PMC1319969
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  • Journal Article

Summary

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The research paper discusses methods for accurately and efficiently separating equine peripheral blood cells into their constituent components – red cells, leukocytes, and platelets.

Experimental Procedures

  • The study focused on the separation of peripheral blood cells of Standard bred horses. These cells circulate throughout the horse’s body, carrying out various functions such as oxygen transport and immune responses.
  • The research introduced methods aimed at separating these peripheral blood cells into their three key components: red blood cells (which carry oxygen), leukocytes (which are part of the immune system), and platelets (which are involved in clotting).

Results and Observations

  • The separation process was not only precise and efficient, but also it had no negative effects on the morphology, or form and structure, of the separated cells. This means the process did not damage or alter the cells in any detrimental way.
  • The differential count of the separated granulocytes and lymphocytes was unchanged from the original sample. Granulocytes and lymphocytes are types of leukocytes, which means the distribution of these types of white blood cells remained consistent after separation.

Significance and Implications

  • The procedure holds promise for its potential use in various areas of equine medicine and research. For instance, it could aid in studying diseases that affect certain types of blood cells, allowing for more targeted treatments.
  • Additionally, the preservation of the normal cell morphology and differential count means that the separated cells retain their original functionality and proportions. This would allow for accurate testing and evaluation in experimental scenarios.

Cite This Article

APA
Carter EI, Valli VE, McSherry BJ. (1974). The separation of peripheral blood cells of the horse. Can J Comp Med, 38(1), 72-74.

Publication

ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 38
Issue: 1
Pages: 72-74

Researcher Affiliations

Carter, E I
    Valli, V E
      McSherry, B J

        MeSH Terms

        • Animals
        • Blood Cells
        • Blood Platelets
        • Cell Separation
        • Centrifugation
        • Edetic Acid
        • Erythrocytes
        • Horses / physiology
        • Leukocytes
        • Methods

        References

        This article includes 2 references
        1. Valli VE, Hulland TJ, McSherry BJ, Robinson GA, Gilman JP. The kinetics of haematopoiesis in the calf. I. An autoradiographical study of myelopoiesis in normal, anaemic and endotoxin treated calves.. Res Vet Sci 1971 Nov;12(6):535-50.
          pubmed: 5145350
        2. Garvin JE. Effects of divalent cations on adhesiveness of rat polymorphonuclear neutrophils in vitro.. J Cell Physiol 1968 Dec;72(3):197-212.
          pubmed: 5724570doi: 10.1002/jcp.1040720307google scholar: lookup

        Citations

        This article has been cited 2 times.
        1. Carter EI, Valli VE, McSherry BJ, Milne FJ, Robinson GA, Lumsden JH. The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse. Can J Comp Med 1974 Jul;38(3):303-13.
          pubmed: 4277706
        2. Targowski SP. Separation of mononuclear leukocytes and polymorphonuclear leukocytes from equine blood. Can J Comp Med 1976 Jul;40(3):285-90.
          pubmed: 1087178