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Home / Equine Research Bank / J Proteomics / The synovial fluid proteome differentiates between septic an...
Journal of proteomics2019; 202; 103370; doi: 10.1016/j.jprot.2019.04.020

The synovial fluid proteome differentiates between septic and nonseptic articular pathologies.

Abstract: Articular conditions are common in horses and can result in loss of function, chronic pain and/or inability to work. Common conditions include osteoarthritis, osteochondrosis and synovial sepsis, which can be life-threatening, but despite the high clinical prevalence of these conditions, rapid and specific diagnosis, monitoring and prognostication remains a challenge for practicing veterinarians. Synovial fluid from a range of arthropathies was enriched for low abundance proteins using combinatorial peptide ligand ProteoMiner™ beads and analysed via liquid chromatography-tandem mass spectrometry. Changes in protein abundances were analysed using label-free quantification. Principle component analysis of differentially expressed proteins identified groupings associated with joint pathology. Findings were validated using ELISA. Lactotransferrin (LTF) abundance was increased in sepsis compared to all other groups and insulin-like growth factor-binding protein 6 (IGFBP6) abundance decreased in sepsis compared to other disease groups. Pathway analysis identified upregulation of the complement system in synovial joint sepsis and the downregulation of eukaryotic translation initiation factors and mTOR signalling pathways in both OA and OC compared to the healthy group. Overall, we have identified a catalogue of proteins which we propose to be involved in osteoarthritis, osteochondrosis and synovial sepsis pathogenesis. SIGNIFICANCE: Osteoarthritis, osteochondrosis and synovial sepsis, which can be life-threatening, are common articular conditions in which rapid and specific diagnosis, monitoring and prognostication remains a challenge for practicing veterinarians. This study has identified that the equine synovial fluid proteome exhibits distinctive profile changes between osteoarthritis, osteochondrosis, synovial sepsis and healthy joints. Elevated synovial abundance of lactotransferrin and decreased insulin-like growth factor-binding protein 6 were both found to distinguish synovial sepsis from all other study groups. Thus, these protein markers may have a future role in clinical practice to enable an earlier and reliable diagnosis of synovial sepsis.
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
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Publication Date: 2019-04-25 PubMed ID: 31028944PubMed Central: PMC6549134DOI: 10.1016/j.jprot.2019.04.020Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focused on identifying clear distinction between septic and nonseptic joint conditions in horses by studying the synovial fluid proteome. The team found unique protein markers that could aid in the earlier and more reliable diagnosis of synovial sepsis.

Objective and Methodology of the Study

  • The study was aimed at discerning between septic and nonseptic joint conditions in horses, such as osteoarthritis, osteochondrosis and synovial sepsis. The current challenge for veterinarians is an efficient and exact diagnosis, monitoring, and prognosis of these conditions.
  • Synovial fluid from different arthropathies was enriched using ProteoMiner™ beads (designed for low abundance proteins) and was analyzed via liquid chromatography-tandem mass spectrometry.
  • The researchers analyzed the change in protein abundances using label-free quantification. By performing a principle component analysis of the differentially expressed proteins, the researchers were able to associate specific groupings with joint pathologies.

Findings of the Study

  • The study observed that Lactotransferrin (LTF) abundance was notably increased in sepsis as compared to all other groups whereas the abundance of insulin-like growth factor-binding protein 6 (IGFBP6) was decreased in sepsis compared to other disease groups.
  • In addition to this, an upregulation of the complement system in synovial joint sepsis was observed.
  • Downregulation of eukaryotic translation initiation factors and mTOR signalling pathways was observed in both osteoarthritis (OA) and osteochondrosis (OC) when compared to the healthy group. This indicates a possible involvement of these pathways in the pathogenesis of these conditions.
  • Overall, the study identified a catalogue of proteins hypothesized to be involved in the pathogenesis of osteoarthritis, osteochondrosis and synovial sepsis.

Significance of the Research

  • This research could be groundbreaking for veterinary medicine in terms of diagnosing equine joint conditions like osteoarthritis, osteochondrosis and synovial sepsis.
  • The findings suggest distinct changes in the synovial fluid protein composition between healthy joints and those afflicted with the aforementioned diseases. The elevated presence of Lactotransferrin and reduced levels of insulin-like growth factor-binding protein 6 were found to distinguish synovial sepsis from the other conditions.
  • This distinction could provide veterinary professionals with clear, biological markers for the early and reliable diagnosis of synovial sepsis, improving prognosis and treatment outcomes.

Cite This Article

APA
Anderson JR, Smagul A, Simpson D, Clegg PD, Rubio-Martinez LM, Peffers MJ. (2019). The synovial fluid proteome differentiates between septic and nonseptic articular pathologies. J Proteomics, 202, 103370. https://doi.org/10.1016/j.jprot.2019.04.020

Publication

Journal of proteomics
ISSN: 1876-7737
NlmUniqueID: 101475056
Country: Netherlands
Language: English
Volume: 202
Pages: 103370

Researcher Affiliations

Anderson, James R
  • Institute of Ageing and Chronic Disease, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK. Electronic address: janders@liverpool.ac.uk.
Smagul, Aibek
  • Institute of Ageing and Chronic Disease, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK. Electronic address: aibek@liverpool.ac.uk.
Simpson, Deborah
  • Centre for Proteome Research, Institute of Integrative Biology, Biosciences Building, Crown Street, University of Liverpool, Liverpool L69 7ZB, UK. Electronic address: dsimpson@liverpool.ac.uk.
Clegg, Peter D
  • Institute of Ageing and Chronic Disease, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK. Electronic address: pclegg@liverpool.ac.uk.
Rubio-Martinez, Luis M
  • Department of Equine Clinical Studies, Institute of Veterinary Science, Chester High Road, Neston CH64 7TE, UK. Electronic address: lrubio@liverpool.ac.uk.
Peffers, Mandy J
  • Institute of Ageing and Chronic Disease, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK. Electronic address: peffs@liv.ac.uk.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Horse Diseases / metabolism
  • Horses / metabolism
  • Osteoarthritis / metabolism
  • Osteoarthritis / veterinary
  • Proteomics
  • Synovial Fluid / metabolism

Grant Funding

  • Wellcome Trust
  • MR/P020941/1 / Medical Research Council

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