The use of a bovine plasma progesterone ELISA kit to measure progesterone in equine, ovine and canine plasmas.

This research article discusses assessing the efficiency of a bovine plasma progesterone kit in measuring progesterone levels in the plasma of horses, sheep, and dogs. The researchers found the kit to be effective, especially in detecting lower concentrations and changes in progesterone levels during the oestrous cycle in these species.

Assessment of the ELISA Kit

• The primary goal of this study was to evaluate the efficiency of an ELISA kit, initially designed to measure progesterone in bovine plasma, in measuring progesterone levels in the plasma of other species, namely horses, sheep, and dogs.
• There was a stipulation against using an immunoassay designed for one species to measure progesterone in the plasma of other species without appropriate validation. Hence, this validation process was essential.
• The criteria used for this assessment included the parallelism to a standard curve, recovery of added progesterone, correlation with an established radioimmunoassay, and the detection of physiological change for each species tested.

Results of the Assessment

• The research process found that the ELISA kit was satisfactory for analyzing the progesterone in the plasma of each of these species.
• The kit was particularly effective in detecting low concentrations of progesterone found during the follicular phase as well as the subsequent rise during the luteal phase of the oestrous cycle.
• This indicates that the use of the kit could be potentially extended beyond bovines and could be essential in hormonal studies and therapies in other animals like horses, sheep, and dogs.