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Home / Equine Research Bank / Parasitol Res / The utility of an rTeGM6-4r-based immunochromatographic test...
Parasitology research2018; 117(9); 2913-2919; doi: 10.1007/s00436-018-5982-8

The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.

Abstract: Our previous studies report epidemics of non-tsetse-transmitted equine trypanosomosis in Mongolia. However, the current status of non-tsetse-transmitted equine trypanosomosis endemicity remains to be clarified in some parts of Mongolia. We previously reported the potential application of rTeGM6-4r-based diagnostic tools, an rTeGM6-4r-based immunochromatographic test (ICT) and an enzyme-linked immunosorbent assay (ELISA), in the serological surveillance of equine trypanosomosis in Mongolia. In the present study, the utility of the rTeGM6-4r-based ICT was validated. The rTeGM6-4r-based ICT accurately diagnosed positive reference sera that had been prepared from dourine horses in Mongolia, similarly to the rTeGM6-4r-based ELISA. The diagnostic performance of the rTeGM6-4r-based ICT was maintained when the strips were preserved for at least 2 months under dry conditions. The ICT detected 42 positive serum samples from a total of 1701 equine sera that had been collected from all 21 provinces of Mongolia. The κ-value, sensitivity and specificity of rTeGM6-4r-based ICT were 0.58, 50.0% (95% CI, 37.7-62.3%) and 99.3% (95% CI, 98.7-99.6%), respectively, in comparison to the rTeGM6-4r-based ELISA. Our field-friendly rTeGM6-4r-based ICT was found to be useful for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.
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Publication Date: 2018-06-25 PubMed ID: 29943319DOI: 10.1007/s00436-018-5982-8Google Scholar: Lookup
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  • Journal Article
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study aimed at testing the effectiveness of rTeGM6-4r-based immunochromatographic test (ICT) in diagnosing non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia. The study found this method to be valid and accurate, making it handy for serological diagnosis in rural Mongolia.

Research Context and Aim

  • The study was conducted in light of previously reported epidemics of non-tsetse-transmitted equine trypanosomosis in Mongolia.
  • In order to get a clearer picture of the disease’s current status in Mongolia, an improved and convenient method of diagnosis was required.
  • The researchers sought to validate the use of rTeGM6-4r-based diagnostic methods, specifically the ICT, for screening equine trypanosomosis.

Methods and Findings

  • Two types of rTeGM6-4r-based diagnostic tools, an ICT and an enzyme-linked immunosorbent assay (ELISA), were previously reported to have potential use in diagnosing equine trypanosomosis.
  • In this study, the ICT test was confirmed as valid and reliable for spotting the disease. It was capable of accurately diagnosing positive samples that had been prepared from horses with the disease in Mongolia. It performed similarly well as the ELISA test.
  • The test strips for ICT performed well even after being preserved for two months under dry conditions, indicating good shelf-life.
  • Out of a total of 1701 equine sera collected from all 21 provinces of Mongolia, the ICT detected 42 positive serum samples.
  • The diagnostic performance and reliability of the ICT were confirmed through statistical measures: its κ-value was 0.58 and it had a sensitivity of 50.0%. Its specificity, i.e., its ability to exclude those without the disease, was commendably high at 99.3%.

Implications of Research

  • This research validates the application of rTeGM6-4r-based ICT for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas, which often lack sophisticated lab facilities.
  • Such an easy-to-use, accurate, field-friendly and reliable diagnostic tool can assist in controlling the disease spread by targeting appropriate treatment and management strategies to affected animals.

Cite This Article

APA
Mizushima D, Amgalanbaatar T, Davaasuren B, Molefe NI, Battur B, Battsetseg B, Inoue N, Yokoyama N, Suganuma K. (2018). The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia. Parasitol Res, 117(9), 2913-2919. https://doi.org/10.1007/s00436-018-5982-8

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 117
Issue: 9
Pages: 2913-2919

Researcher Affiliations

Mizushima, Daiki
  • National Research Center for Protozoan Diseases, OIE Reference Laboratory for Surra, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
Amgalanbaatar, Tovuu
  • Institute of Veterinary Medicine, Laboratory of Molecular Genetics, Mongolian University of Life Sciences, Zaisan, Ulaanbaatar, 17024, Mongolia.
Davaasuren, Batdorj
  • National Research Center for Protozoan Diseases, OIE Reference Laboratory for Surra, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
  • Institute of Veterinary Medicine, Laboratory of Molecular Genetics, Mongolian University of Life Sciences, Zaisan, Ulaanbaatar, 17024, Mongolia.
Molefe, Nthatisi Innocentia
  • National Research Center for Protozoan Diseases, OIE Reference Laboratory for Surra, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
Battur, Banzragch
  • Institute of Veterinary Medicine, Laboratory of Molecular Genetics, Mongolian University of Life Sciences, Zaisan, Ulaanbaatar, 17024, Mongolia.
Battsetseg, Badgar
  • Institute of Veterinary Medicine, Laboratory of Molecular Genetics, Mongolian University of Life Sciences, Zaisan, Ulaanbaatar, 17024, Mongolia.
Inoue, Noboru
  • Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
Yokoyama, Naoaki
  • National Research Center for Protozoan Diseases, OIE Reference Laboratory for Surra, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
Suganuma, Keisuke
  • National Research Center for Protozoan Diseases, OIE Reference Laboratory for Surra, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan. k.suganuma@obihiro.ac.jp.
  • Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan. k.suganuma@obihiro.ac.jp.

MeSH Terms

  • Animals
  • Chromatography, Affinity / methods
  • Enzyme-Linked Immunosorbent Assay / methods
  • Horse Diseases / diagnosis
  • Horse Diseases / parasitology
  • Horse Diseases / transmission
  • Horses / parasitology
  • Immunologic Tests / methods
  • Mongolia
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Rural Population
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Trypanosomiasis / diagnosis
  • Trypanosomiasis / veterinary
  • Variant Surface Glycoproteins, Trypanosoma / genetics
  • Variant Surface Glycoproteins, Trypanosoma / immunology

Grant Funding

  • 17jm0110006h0005 / Japan Agency for Medical Research and Development (JP)

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Citations

This article has been cited 3 times.
  1. Verney M, Gautron M, Lemans C, Rincé A, Hans A, Hébert L. Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis.. Sci Rep 2022 Jan 25;12(1):1308.
    doi: 10.1038/s41598-022-05356-ypubmed: 35079068google scholar: lookup
  2. Elhosseiny NM, Samir TM, Ali AA, El-Kholy AA, Attia AS. Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis.. Pharmaceutics 2021 Jul 26;13(8).
    doi: 10.3390/pharmaceutics13081141pubmed: 34452099google scholar: lookup
  3. Mizushima D, Amgalanbaatar T, Davaasuren B, Kayano M, Naransatsral S, Myagmarsuren P, Otgonsuren D, Enkhtaivan B, Davkharbayar B, Mungun-Ochir B, Baatarjargal P, Nyamdolgor U, Soyolmaa G, Altanchimeg A, Zoljargal M, Nguyen TT, Battsetseg B, Battur B, Inoue N, Yokoyama N, Suganuma K. Nationwide serological surveillance of non-tsetse-transmitted horse trypanosomoses in Mongolia.. Parasite Epidemiol Control 2020 Aug;10:e00158.
    doi: 10.1016/j.parepi.2020.e00158pubmed: 32642568google scholar: lookup
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