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Animals : an open access journal from MDPI2024; 14(4); 583; doi: 10.3390/ani14040583

The Welfare of Horses Competing in Three-Barrel Race Events Is Shown to Be Not Inhibited by Short Intervals between Starts.

Abstract: Equestrian sports require precise animal welfare and health evaluations. To test the hypothesis that horses maintain their welfare when subjected to two three-barrel (3TB) races with 2 min intervals, an experiment was designed to evaluate their surface temperature using infrared thermography (IRT) in regions of interest (barrel, flank, neck, jaw, corner of the mouth, and ocular caruncle) and also measure blood biomarkers (hemogram, total plasma protein, fibrinogen, urea, creatinine, GGT, CK, cortisol, IL-6, and IL-1β). Ten Quarter Horses were monitored through thermography (pre-race, +1, +4, and +24 h post-race) and blood sampling (pre-race, +1, +4, and +24 h post-race). ANOVA and Tukey test at 5% were used. IRT in six regions of interest (Left/Right-barrel, flank, neck muscles) increased at +, with no differences between values recorded at +1 and +4 when compared to those measured pre-race ( > 0.05). Plasma protein, RBC count, hemoglobin, hematocrit, WBC count, neutrophils, and lymphocytes ( 0.05). Results indicate that well-conditioned 3TB horses subjected to two races at short intervals do not show changes that could be related to impaired health or welfare.
Publication Date: 2024-02-09 PubMed ID: 38396551PubMed Central: PMC10886278DOI: 10.3390/ani14040583Google Scholar: Lookup
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  • Journal Article

Summary

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This research investigates the welfare of horses competing in multiple three-barrel (3TB) races with short breaks in between, concluding that such events do not appear to harm the health or general wellbeing of the animals.

Study Design

  • The study was carried out to examine the impact of two consecutive 3TB horse races with a short, 2-minute interval between them on the welfare of the competing horses.
  • Ten Quarter Horses were used in this experiment. Their wellbeing was evaluated using infrared thermography (IRT) to measure the animals’ surface temperature in several regions of interest, namely the barrel, flank, neck, jaw, corner of the mouth, and ocular caruncle. Additionally, a range of blood biomarkers was analyzed.
  • These measures were taken at four instances: prior to the start of the races (pre-race), 1 hour after the race (+1), 4 hours post-race (+4), and 24 hours after the race (+24).

Key Findings

  • The researchers found that the IRT increased immediately after the race (+) in six regions of interest including the left and right paradernal region, flank, and neck muscles. However, there was no significant difference in the measurements recorded 1 and 4 hours after the race in comparison to the pre-race values. This suggests that surface temperature elevations due to the race are momentary and normalize within an hour.
  • The blood biomarkers showed a similar pattern. Certain metrics such as plasma protein, RBC count, hemoglobin, hematocrit, WBC count, neutrophils, and lymphocytes increased immediately after the race, but returned to normal levels within an hour.
  • Critical biomarkers related to stress and inflammation such as cortisol, IL-6, and IL-1β did not show any significant changes after the race. Typical increases in these markers would indicate physiological stress, inflammation, or potential harm to the animal.

Conclusion

  • The study concluded that well-conditioned Quarter Horses do not show any significant changes that would indicate impaired health or animal welfare, even when they are subjected to two 3TB races with only a short interval in between.
  • The rapid return to normal values after initial increases suggests that these physical activities do not cause lasting harm or distress to the animals, thus supporting the hypothesis that horse welfare is sustained in such race conditions.

Cite This Article

APA
Filho HCM, Trindade KLG, Silva CJFL, Cruz RKS, Vilela CF, Coelho CS, Filho JDR, Manso HECCC. (2024). The Welfare of Horses Competing in Three-Barrel Race Events Is Shown to Be Not Inhibited by Short Intervals between Starts. Animals (Basel), 14(4), 583. https://doi.org/10.3390/ani14040583

Publication

ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 4
PII: 583

Researcher Affiliations

Filho, Helio C Manso
  • Núcleo de Pesquisa Equina, Universidade Federal Rural de Pernambuco (UFRPE), Recife 52171-900, PE, Brazil.
Trindade, Keity L G
  • Núcleo de Pesquisa Equina, Universidade Federal Rural de Pernambuco (UFRPE), Recife 52171-900, PE, Brazil.
Silva, Carolina J F L
  • Núcleo de Pesquisa Equina, Universidade Federal Rural de Pernambuco (UFRPE), Recife 52171-900, PE, Brazil.
Cruz, Raissa K S
  • Faculdade de Medicina Veterinária, Centro Universitário Cesmac, Maceió 57051-160, AL, Brazil.
Vilela, César F
  • Independent Researcher, Americana 13474-470, SP, Brazil.
Coelho, Clarisse S
  • Mediterranean Institute for Agriculture, Environment and Development, Universidade de Évora, 7006-554 Évora, Portugal.
  • Veterinary and Animal Research Centre (CECAV), Faculty of Veterinary Medicine, Lusofona University, 376 Campo Grande, 1749-024 Lisbon, Portugal.
Filho, José D Ribeiro
  • Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Manso, Helena E C C C
  • Núcleo de Pesquisa Equina, Universidade Federal Rural de Pernambuco (UFRPE), Recife 52171-900, PE, Brazil.

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 48 references
  1. Souza T.M.S., Rêgo G.M., Nunes G.S., Paraguaio P.E., Machado L.P.. Elevação transitória da atividade sérica das enzimas musculares em equinos após exercício de vaquejada. Cien. Vet. Trop. 2014;17:56–57.
  2. Silva C.J.F.L., Trindade K.L.G., Cruz R.K.S., Vilela C.F., Coelho C.S., Ribeiro Filho J.D., Manso H.H.C.C.C., Manso Filho H.C.. Association between infrared thermography, blood count and creatine kinase in the evaluation of the welfare of vaquejada horses. Open J. Vet. Med. 2023;13:53–67.
    doi: 10.4236/ojvm.2023.136006google scholar: lookup
  3. Lo Feudo C.M., Stucchi L., Conturba B., Stancari G., Zucca E., Ferrucci F.. Medical causes of poor performance and their associations with fitness in Standardbred racehorses. J. Vet. Intern. Med. 2023;37:1514–1527.
    doi: 10.1111/jvim.16734pmc: PMC10365054pubmed: 37148147google scholar: lookup
  4. Witkowska-Piłaszewicz O.D., Zmigrodzka M., Winnicka A., Miskiewicz A., Strzelec K., Cywinska A.. Serum amyloid A in equine health and disease. Equine Vet. J. 2019;51:293–298.
    doi: 10.1111/evj.13062pmc: PMC7163734pubmed: 30565319google scholar: lookup
  5. Voigt M.A., Hiney K., Richardson J.C., Waite K., Borron A., Brady C.M.. Show Horse Welfare: Horse Show Competitors’ Understanding, Awareness, and Perceptions of Equine Welfare. J. Appl. Anim. Welf. Sci. 2016;19:335–352.
    doi: 10.1080/10888705.2016.1152190pubmed: 27029609google scholar: lookup
  6. Mann S., Ramsay J.D., Wakshlag J.J., Stokol T., Reed S., Divers T.J.. Investigating the pathogenesis of high-serum gamma-glutamyl transferase activity in Thoroughbred racehorses: A series of case-control studies. Equine Vet. J. 2022;54:39–51.
    doi: 10.1111/evj.13435pubmed: 33555643google scholar: lookup
  7. Holtby A.R., McGivney B.A., Browne J.A., Katz L.M., Murphy K.J., Hill E.W.. Variation in salivary cortisol responses in yearling Thoroughbred racehorses during their first year of training. PLoS ONE 2023;18:e0284102.
  8. Atock M.A., Williams R.B.. Welfare of competition horses. Rev. Sci. Et Tech. (Int. Off. Epizoot.) 1994;13:217–232.
    doi: 10.20506/rst.13.1.765pubmed: 8173097google scholar: lookup
  9. Andriichuk A., Tkachenko H., Tkachova I.. Oxidative Stress Biomarkers and Erythrocytes Hemolysis in Well-Trained Equine Athletes Before and After Exercise. J. Equine Vet. Sci. 2016;36:32–43.
  10. Mach N., Ruet A., Clark A., Bars-Cortina D., Ramayo-Caldas Y., Crisci E., Pennarun S., Dhorne-Pollet S., Foury A., Moisan M.-P.. Priming for welfare: Gut microbiota is associated with equitation conditions and behavior in horse athletes. Sci. Rep. 2020;10:8311.
    doi: 10.1038/s41598-020-65444-9pmc: PMC7239938pubmed: 32433513google scholar: lookup
  11. Gold J.R., Knowles D.P., Coffey T., Bayly W.M.. Exercise-induced pulmonary hemorrhage in barrel racing horses in the Pacific Northwest region of the United States. J. Vet. Intern. Med. 2018;32:839–845.
    doi: 10.1111/jvim.15066pmc: PMC5866954pubmed: 29460489google scholar: lookup
  12. Souza L.A., Hunka M.M., Nery P.C.R., Coelho C.S., Manso H.E.C.C.C., Manso Filho H.C.. The effect of repeated barrel racing on blood biomarkers and physiological parameters in Quarter Horses. Comp. Exerc. Physiol. 2018;14:47–54.
    doi: 10.3920/CEP170019google scholar: lookup
  13. Scott M.. Musculoskeletal injuries in nonracing Quarter Horses. Vet. Clin. Equine. 2008;24:133–152.
    doi: 10.1016/j.cveq.2007.11.006pubmed: 18314040google scholar: lookup
  14. Medica P., Cravana C., Fazio E., Ferlazzo A.. Hormonal responses of Quarter Horses to a 6-week conventional Western-riding training programe. Livest. Sci. 2011;140:262–267.
  15. Sala L.C.C., Elui M.C., Jardin M.C.. Avaliação termográfica da musculatura pélvica de equinos da modalidade esportiva de três tambores. PUBVET 2012;6:1437.
  16. Rodrigues I.M.S.M.M., Spindola B.F., Botteon P.T.L.. Perfil bioquímico e oxidativo de cavalos usados em prova simulada dos três tambores. Rev. Bras. Med. Vetet. 2016;38:93–100.
    doi: 10.2430/00000000000000google scholar: lookup
  17. Gomes C.L.N., Alves A.M., Ribeiro Filho J.D., Moraes Junior F.J., Barreto Junior R.A., Fucuta R.S., Ribeiro B.M., Miranda L.M.. Physiological and biochemical responses and hydration status in equines after two-barrel racing courses. Pesq. Vet. Bras. 2020;40:992–1001.
  18. Holtby A.R., Hall T.J., Han H., Murhy K.J., MacHugh D.E., Katz L.M., Hill E.W.. Integrative genomics analysis highlights functionally relevant genes for equine behaviour. Anim. Genet. 2023:1–13.
    doi: 10.1111/age.13320pubmed: 36971191google scholar: lookup
  19. Hunka M.M., Souza L.A., Almeida T.H.S., Nery P.C.R., Manso H.E.C.C.C., Manso Filho H.C.. Metabolic and physiological changes during and after vaquejada exercise in horse. Med. Veterinária (UFRPE) 2018;12:254–262.
  20. Liburt N.R., Adams A., Betancourt A., Horohov D.W., McKeever K.H.. Exercise-induced increases in inflammatory cytokines in muscle and blood of horses. Equine Vet. J. 2010;42:280–288.
  21. Brownlow M., Smith T.. The use of the hand-held infrared thermometer as an early detection tool for exertional heat illness in Thoroughbred racehorses: A study at racetracks in eastern Australia. Equine Vet. Educ. 2020;33:296–305.
    doi: 10.1111/eve.13299google scholar: lookup
  22. Witkowska-Piłaszewicz O., Masko M., Domino M., Winnicka A.. Infrared thermography correlates with lactate concentration in blood during race training in horses. Animals 2020;10:2072.
    doi: 10.3390/ani10112072pmc: PMC7695344pubmed: 33182281google scholar: lookup
  23. Kędzierski W., Cywińska A.. The Effect of Different Physical Exercise on Plasma Leptin, Cortisol, and Some Energetic Parameters Concentrations in Purebred Arabian Horses. J. Equine Vet. Sci. 2014;34:1059–1063.
  24. Bartolomé E., Sánchez M.J., Molina A., Schaefer A.L., Cervantes I., Valera M.. Using eye temperature and heart rate for stress assessment in young horses competing in jumping competitions and its possible influence on sport performance. Animal 2013;7:2044–2053.
    doi: 10.1017/S1751731113001626pubmed: 24067493google scholar: lookup
  25. Valera M., Bartolomé E., Sánchez M.J., Molina A., Cook N., Schaefer A.L.. Changes in Eye Temperature and Stress Assessment in Horses During Show Jumping Competitions. J. Equine Vet. Sci. 2012;32:827–830.
  26. ABQM—Associação Brasileira de Criadores de Cavalo Quarto de Milha. Regras Para Provas Dos Três Tambores. ABQM; São Paulo, Brazil: 2020. 175p.
  27. Mellor D.J.. Operational details of the five domains model and its key applications to the assessment and management of animal welfare. Animals 2017;7:60.
    doi: 10.3390/ani7080060pmc: PMC5575572pubmed: 28792485google scholar: lookup
  28. Turner T.A.. Diagnostic thermography. Vet. Clin. N. Am. 2001;17:95–113.
    doi: 10.1016/S0749-0739(17)30077-9pubmed: 11488048google scholar: lookup
  29. Bartolomé E., Perdomo-González D.I., Sánchez-Guerrero M.J., Valera M.. Genetic parameters of effort and recovery in sport horses assessed with infrared thermography. Animals 2021;11:832.
    doi: 10.3390/ani11030832pmc: PMC8001494pubmed: 33809482google scholar: lookup
  30. Kim S.-M., Cho G.-J.. Validation of eye temperature assessed using infrared thermography as an indicator of welfare in horses. Appl. Sci. 2021;11:7186.
    doi: 10.3390/app11167186google scholar: lookup
  31. Kruljc P.. Thermographic examination of the horse. Acta Vet.-Beograd. 2023;73:289–316.
    doi: 10.2478/acve-2023-0023google scholar: lookup
  32. NOAA—National Oceanic Atmospheric Administration. Livestock Hot Weather Stress. US Department of Commerce, National Oceanic and Atmospheric Administration, National Weather Service Central Region; Kansas City, MO, USA: 1976. Regional Operations Manual Letter C-31-76.
  33. Loving N.S., Johnston A.M.. Veterinary Manual for the Performance Horse. Blackwell Science Ltd.; Hoboken, NJ, USA: 1995. 580p.
  34. Cymbaluk N.F., Christison G.I.. Environmental Effects on Thermoregulation and Nutrition of Horses. Vet. Clin. N. Am. 1990;6:255–372.
    doi: 10.1016/S0749-0739(17)30546-1pubmed: 2202497google scholar: lookup
  35. Vitali A., Segnalini M., Bertocchi L., Bernabucci U., Nardone A., Lacetera N.. Seasonal pattern of mortality and relationships between mortality and temperature-humidity index in dairy cows. J. Dairy Sci. 2009;92:3781–3790.
    doi: 10.3168/jds.2009-2127pubmed: 19620660google scholar: lookup
  36. Gabay C., Kushner I.. Acute-phase protein, and other systemic responses to inflammation. N. Engl. J. Med. 1999;11:448–454.
    doi: 10.1056/NEJM199902113400607pubmed: 9971870google scholar: lookup
  37. Peng S., Magdesian K.G., Dowd J., Carpenter R., Ho W., Finno C.J.. Investigation of high gamma-glutamyltransferase syndrome in California Thoroughbred racehorses. J. Vet. Intern. Med. 2022;36:2203–2212.
    doi: 10.1111/jvim.16582pmc: PMC9708438pubmed: 36377652google scholar: lookup
  38. Borges A.S., Divers T.J., Stokol T., Mohammed O.H.. Serum Iron and Plasma Fibrinogen Concentrations as Indicators of Systemic Inflammatory Diseases in Horses. J. Vet. Intern. Med. 2007;21:489–494.
  39. Page A.E., Stewart J.C., Holland R.E., Horohov D.W.. The Impact of Training Regimen on the Inflammatory Response to Exercise in 2-Year-Old Thoroughbreds. J. Equine Vet. Sci. 2017;58:78–83.
  40. Fazio E., Lindner A., Wegener J., Medica P., Hartmann U., Ferlazzo A.. Plasma cortisol concentration during standardized exercise in Standardbred racehorses within a racing season. Pferdeheilkunde 2023;39:151–157.
    doi: 10.21836/PEM20230204google scholar: lookup
  41. Rocha A.L., Pinto A.P., Kohama E.B., Pauli J.R., Moura L.P., Cintra D.E., Ropelle E.R., Silva A.S.R.. The proinflammatory effects of chronic excessive exercise. Cytokine 2019;119:57–61.
    doi: 10.1016/j.cyto.2019.02.016pubmed: 30884427google scholar: lookup
  42. Stallones L., McManus P., McGreevy P.. Sustainability and the Thoroughbred Breeding and Racing Industries: An Enhanced One Welfare Perspective. Animals 2023;13:490.
    doi: 10.3390/ani13030490pmc: PMC9913237pubmed: 36766378google scholar: lookup
  43. Trindade P.H.E., Ferraz G.C., Lima M.L.P., Negrão J.A., Costa M.J.R.P.. Eye surface temperature as a potential indicator of physical fitness in ranch horses. JEVS 2019;75:1–8.
    doi: 10.1016/j.jevs.2018.11.015pubmed: 31002082google scholar: lookup
  44. Verdegaal E.-L.J.M.M., Howarth G.S., McWhorter T.J., Delesalle C.J.G.. Is continuous monitoring of skin surface temperature a reliable proxy to assess the thermoregulatory response in endurance horses during field exercise?. Front. Vet. Sci. 2022;9:894146.
    doi: 10.3389/fvets.2022.894146pmc: PMC9196037pubmed: 35711810google scholar: lookup
  45. Hodgson D.R., McGowanm C.M., McKeever K.H.. The Athletic Horse: Principles and Practice of Equine Sports Medicine. Saunders; Philadelphia, PA, USA: 2014. 408p.
  46. Lisboa B.R.F., Silva J.A.R., Silva W.C., Barbosa A.V.C., Silva L.K.X., Lourenço-Júnior J.D.B.. Evaluation of thermoregulation of horses (Equus caballus) submitted to two methods of post-exercise cooling, in hot and humid climate conditions, in the Eastern Amazon. Front. Vet. Sci. 2023;10:1150763.
    doi: 10.3389/fvets.2023.1150763pmc: PMC10149968pubmed: 37138916google scholar: lookup
  47. Stewart M., Schaefer A.L., Haley B.D., Colyn J., Cook N.J., Stafford K.J., Webster J.R.. Infrared thermography as a non-invasive method for detecting fear-related responses of cattle to handling procedures. Anim. Welf. 2008;17:387–393.
    doi: 10.1017/S0962728600027895google scholar: lookup
  48. Klous L., Siegers E., van den Broek J., Folkerts M., Gerrett N., Sloet van Oldruitenborgh-Oosterbaan M., Munsters C.. Effects of pre-cooling on thermophysiological responses in elite eventing horses. Animals 2020;10:1664.
    doi: 10.3390/ani10091664pmc: PMC7552184pubmed: 32947831google scholar: lookup

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