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American journal of veterinary research2006; 67(4); 669-677; doi: 10.2460/ajvr.67.4.669

Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction.

Abstract: To evaluate time-dependent alterations in gene expression of chemokines in bronchial epithelium of recurrent airway obstruction (RAO)-affected horses and whether alterations resulted from increases in gene expression of interleukin (IL)-17 in cells isolated from bronchoalveolar lavage fluid (BALF). Methods: 8 RAO-susceptible horses and 9 control horses. Methods: In 2 experiments, both groups of horses were evaluated after being maintained on pasture and after being stabled and fed dusty hay for 1, 14, 35, and 49 days (experiment 1) or 14 and 28 days (experiment 2). In experiment 1, gene expression of IL-8, chemokine (C-X-C motif) ligand 1 (CXCL1), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and Toll-like receptor 4 (TLR4) in epithelium and IL-8, IL-17, and TLR4 in BALF cells was measured. In experiment 2, bronchial biopsy specimens were evaluated for IL-8 immunoreactivity. Results: In RAO-susceptible horses after 14 days of challenge exposure, there was a 3- and 10-fold increase in gene expression of IL-8 for epithelial and BALF cells and an increase in IL-8 immunoreactivity in epithelial cells. Challenge exposure failed to alter gene expression of CXCL1, GM-CSF, G-CSF, and TLR4 in epithelial cells of any horses at any time point. During challenge exposure, gene expression of BALF cell IL-17 was downregulated in control horses (day 1) and upregulated in RAO-affected horses (day 35). Conclusions: Epithelial-derived IL-8 may promote airway neutrophilia, but the inciting stimulus is unlikely to be IL-17 because upregulation of this gene is subsequent to that of IL-8 in epithelial cells.
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Publication Date: 2006-04-04 PubMed ID: 16579761DOI: 10.2460/ajvr.67.4.669Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article details the observation of time-specific changes in gene expression related to chemokines in the epithelium of the bronchii in horses suffering from recurrent airway obstruction (RAO). It specifically explores how changes in interleukin (IL)-17 gene expression may impact these alterations.

Methodology

  • The study included 8 horses susceptible to RAO and another 9 healthy horses set as controls.
  • Two experiments were conducted; one observed horses after being maintained on a pasture and after being exposed to low-quality, dusty feed for a sequence of days (1, 14, 35 and 49), the second experiment studied the same conditions but with a duration of just 14 and 28 days.
  • During the first experiment, the expressions of multiple genes such as IL-8, CXCL1 chemokine, GM-CSF, G-CSF, and TLR4 in the bronchial epithelium were measured. Similarly, the expressions of IL-8, IL-17, and TLR4 in BALF cells were noted.
  • In the second experiment, bronchial biopsy specimens were assessed and analyzed for IL-8 immunoreactivity.

Results

  • It was noted that RAO-prone horses exhibited a significant increase (3 to 10 fold) in IL-8 gene expression in both the bronchial epithelial and BALF cells after 14 days of exposure to dusty hay.
  • Additionally, IL-8 immunoreactivity in the bronchial epithelium cells was observed in these horses.
  • However, the exposure to dusty hay did not alter the gene expressions of chemokine CXCL1, GM-CSF, G-CSF, and TLR4 in the epithelial cells of horses, irrespective of their susceptibility to RAO.
  • During exposure to the dusty feed, BALF cell IL-17 gene expression was reduced in control horses (day 1) and significantly increased in RAO affected horses (day 35).

Conclusions

  • The researchers concluded that the gene expression changes in IL-8 can aid in the increased neutrophil infiltration in the airways (neutrophilia). However, IL-17 is unlikely to be the trigger for such gene expression changes as the upregulation of IL-17 happens subsequent to IL-8.

Cite This Article

APA
Ainsworth DM, Wagner B, Franchini M, Grünig G, Erb HN, Tan JY. (2006). Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction. Am J Vet Res, 67(4), 669-677. https://doi.org/10.2460/ajvr.67.4.669

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 67
Issue: 4
Pages: 669-677

Researcher Affiliations

Ainsworth, Dorothy M
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Wagner, Bettina
    Franchini, Marco
      Grünig, Gabriele
        Erb, Hollis N
          Tan, Jean-Yin

            MeSH Terms

            • Airway Obstruction / immunology
            • Airway Obstruction / veterinary
            • Animal Feed
            • Animals
            • Bronchi / immunology
            • Bronchoalveolar Lavage Fluid / cytology
            • Disease Susceptibility / veterinary
            • Gene Expression Regulation
            • Horse Diseases / immunology
            • Horses
            • Interleukin-8 / genetics
            • Leukocyte Count / veterinary
            • Lymphocyte Count / veterinary
            • Recurrence
            • Respiratory Mucosa / immunology

            Citations

            This article has been cited 19 times.
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