Tissue-specific expression of the calcium transporter genes TRPV5, TRPV6, NCX1, and PMCA1b in the duodenum, kidney and heart of Equus caballus.
Abstract: Calcium transporter genes, such as transient receptor potential cation channel subfamily V members 5/6 (TRPV5/6), Na(+)/Ca(2+) exchanger 1 (NCX1), and plasma membrane calcium-transporting ATPase 1b (PMCA1b), are essential for maintaining homeostasis and metabolizing Ca(2+) ions. The TRPV5 and TRPV6 proteins play an important role in Ca(2+ )absorption, and NCX1 and PMCA1b are both critical for intracellular calcium homeostasis. In this study, the tissue-specific mRNA and protein expression of these calcium transporter genes in the duodenum, kidney and heart of the horse (Equus caballus) was examined using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. The tissue localization of these calcium transporters was also investigated using immunohistochemistry. The results showed that TRPV5 mRNA was highly expressed in the kidney but was scarce in the duodenum and heart. TRPV6 mRNA levels were similar in all the tissues. NCX1 and PMCA1b were both highly expressed in the heart, but no difference in NCX1 and PMCA1b mRNA expressions was observed in the duodenum and kidney. The aspect of protein expression was similar with mRNA expression data. Localization of calcium transporter genes were detected enterocytes in duodenum, the distal convoluted tubules in the kidney, and within the cardiac muscle cells of the heart. Based on these results, calcium transport genes appear to be expressed in horse tissues at levels similar to those observed in other vertebrates.
Publication Date: 2011-07-07 PubMed ID: 21737966DOI: 10.1292/jvms.11-0141Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates how the expression of calcium transporter genes TRPV5, TRPV6, NCX1, and PMCA1b varies across three different tissues (the duodenum, kidney, and heart) in horses. The results suggest that these genes play a critical role in maintaining calcium balance and are expressed in similar levels as seen in other animals.
About Calcium Transporter Genes
- Calcium transporter genes, including transient receptor potential cation channel subfamily V members 5/6 (TRPV5/6), Na(+)/Ca(2+) exchanger 1 (NCX1), and plasma membrane calcium-transporting ATPase 1b (PMCA1b), play a vital role in calcium homeostasis and metabolizing calcium ions (Ca2+).
- TRPV5 and TRPV6 proteins are involved in Ca2+ absorption, while NCX1 and PMCA1b maintain intracellular calcium homeostasis.
Research Methodology
- The study analysed the tissue-specific mRNA and protein expression of these calcium transporter genes in horses’ three tissues: duodenum, kidney, and heart.
- The researchers used reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis to examine gene expression, and immunohistochemistry to investigate the tissue localization of these calcium transporters.
Key Findings
- The analysis revealed that TRPV5 mRNA was primarily expressed in the kidneys, with little to no expression in the duodenum and heart.
- TRPV6 mRNA was present in all the tissues studied, with similar levels observed across these tissues.
- NCX1 and PMCA1b showed high expression in the heart, but no significant difference in their expression was noticed in the duodenum and kidney.
- The protein expression of these genes matched the mRNA expression data.
- Tissue localization detected these calcium transporter genes in enterocytes in the duodenum, distal convoluted tubules in the kidney, and cardiac muscle cells in the heart.
- The expression levels of these calcium transport genes observed in horse tissues were comparable to those found in other vertebrates.
Cite This Article
APA
Hwang I, Jung EM, Yang H, Choi KC, Jeung EB.
(2011).
Tissue-specific expression of the calcium transporter genes TRPV5, TRPV6, NCX1, and PMCA1b in the duodenum, kidney and heart of Equus caballus.
J Vet Med Sci, 73(11), 1437-1444.
https://doi.org/10.1292/jvms.11-0141 Publication
Researcher Affiliations
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University,Cheongju, Chungbuk 361–763, Republic of Korea.
MeSH Terms
- Animals
- Blotting, Western / veterinary
- Duodenum / metabolism
- Gene Expression
- Horses / genetics
- Horses / metabolism
- Kidney / metabolism
- Male
- Myocardium / metabolism
- Organ Specificity
- Plasma Membrane Calcium-Transporting ATPases / genetics
- Plasma Membrane Calcium-Transporting ATPases / metabolism
- RNA, Messenger / analysis
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Sodium-Calcium Exchanger / genetics
- Sodium-Calcium Exchanger / metabolism
- TRPV Cation Channels / genetics
- TRPV Cation Channels / metabolism
Citations
This article has been cited 6 times.- Gwanyanya A, Mubagwa K. Emerging role of transient receptor potential (TRP) ion channels in cardiac fibroblast pathophysiology. Front Physiol 2022;13:968393.
- Liao QS, Du Q, Lou J, Xu JY, Xie R. Roles of Na(+)/Ca(2+) exchanger 1 in digestive system physiology and pathophysiology. World J Gastroenterol 2019 Jan 21;25(3):287-299.
- Li P, Wang R, Jiao H, Wang X, Zhao J, Lin H. Effects of Dietary Phosphorus Level on the Expression of Calcium and Phosphorus Transporters in Laying Hens. Front Physiol 2018;9:627.
- Azarpeykan S, Dittmer KE, Marshall JC, Perera KC, Gee EK, Acke E, Thompson KG. Evaluation and Comparison of Vitamin D Responsive Gene Expression in Ovine, Canine and Equine Kidney. PLoS One 2016;11(9):e0162598.
- Chen L, Tuo B, Dong H. Regulation of Intestinal Glucose Absorption by Ion Channels and Transporters. Nutrients 2016 Jan 14;8(1).
- Hwang I, Hong EJ, Yang H, Kang HS, Ahn C, An BS, Jeung EB. Regulation of tight junction gene expression in the kidney of calbindin-D9k and/or -D28k knockout mice after consumption of a calcium- or a calcium/vitamin D-deficient diet. BMC Biochem 2014 Mar 15;15:6.
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