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Biology of reproduction2011; 84(5); 872-885; doi: 10.1095/biolreprod.110.088732

Transcriptional profiling of equine conceptuses reveals new aspects of embryo-maternal communication in the horse.

Abstract: Establishment and maintenance of pregnancy are critically dependent on embryo-maternal communication during the preimplantation period. The horse is one of the few domestic species in which the conceptus-derived pregnancy recognition signal has not been identified. To gain new insights into the factors released by the equine conceptus, transcriptional profiling analyses of conceptuses retrieved 8, 10, 12, and 14 days after ovulation were performed using a whole-genome microarray. Selected array data were confirmed using quantitative PCR, and the expression of proteins of interest was confirmed using immunohistochemistry and Western blotting. Gene ontology classification of differentially regulated transcripts underlines the ongoing embryo-maternal dialogue. Transcript showing higher expression levels as conceptus' development proceeds mainly localizes to the extracellular environment, thereby having the potential to act upon the uterine environment. Genes involved in the positive regulation of the immune system are enriched among transcripts displaying decreased expression, reflecting the need of the semiallograft conceptus to be protected from the immune system. A subset of differentially expressed genes, such as BRCA1 and FGF2, has previously been described to be expressed by early stages of embryonic development, whereas other transcripts are apparently unique to equine conceptuses, as their expression has not been reported in other species. These transcripts include fibrinogen subunits, the expressions of which were confirmed at the mRNA and protein level. Furthermore, results indicate the counteraction of trophoblast invasion, and that the conceptus appears to regulate changes in sialic acid content of its capsule, an event suggested to be essential for successful establishment of pregnancy.
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Publication Date: 2011-01-05 PubMed ID: 21209420DOI: 10.1095/biolreprod.110.088732Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the process of embryo-maternal communication in horses, during the preimplantation period of pregnancy, by conducting transcriptional profiling analyses. The researchers analyze the various transcripts and proteins expressed by the developing equine conceptus, revealing new insights into how the horse embryo may signal pregnancy recognition and interact with the uterine environment.

Methodology

  • The study performed transcriptional profiling analyses on equine conceptuses, or embryos, obtained 8, 10, 12, and 14 days after ovulation. This was done using a whole-genome microarray, a chip that carries an array of sequences representing the genome.
  • Selected data from the array were then confirmed through quantitative PCR, a method that measures the amount of a specific RNA.
  • Immunohistochemistry and Western blotting were also used to investigate the expression of certain proteins of interest.

Findings

  • The gene ontology classification, which groups genes based on their shared biological characteristics, highlighted the ongoing dialogue between the embryo and the mother. This largely happens through genes that express more as the embryo develops, particularly those located in the extracellular environment. These genes can potentially impact the uterine environment.
  • The study found that genes responsible for positively regulating the immune system were enriched among the transcripts that exhibited decreased expression levels. This reflects the need for the embryo, which is technically a semiallograft, to protect itself from the mother’s immune system.
  • A subset of the differentially expressed genes, including BRCA1 and FGF2, have been previously identified in early stages of embryonic development in other studies. But there are still other transcripts unique to horse embryos, such as fibrinogen subunits, whose expressions were confirmed at both the mRNA and protein level in this study.

Implications

  • The study’s findings indicate that the conceptus, or early embryo, counters the invasion of the trophoblast, the outer layer of the embryo that provides nutrients from the mother to the fetus and develops into a large part of the placenta.
  • The research suggests that the conceptus regulates changes in its capsule’s sialic acid content—an event considered essential for the successful establishment of pregnancy. This insight could be critical for further investigations into equine reproduction biology.

Cite This Article

APA
Klein C, Troedsson MH. (2011). Transcriptional profiling of equine conceptuses reveals new aspects of embryo-maternal communication in the horse. Biol Reprod, 84(5), 872-885. https://doi.org/10.1095/biolreprod.110.088732

Publication

Biology of reproduction
ISSN: 1529-7268
NlmUniqueID: 0207224
Country: United States
Language: English
Volume: 84
Issue: 5
Pages: 872-885

Researcher Affiliations

Klein, Claudia
  • Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA. claudia.klein@uky.edu
Troedsson, Mats H T

    MeSH Terms

    • Animals
    • BRCA1 Protein / genetics
    • BRCA1 Protein / metabolism
    • Blastocyst / metabolism
    • DNA Probes
    • Embryonic Development
    • Extraembryonic Membranes / cytology
    • Extraembryonic Membranes / metabolism
    • Female
    • Fibrinogen / genetics
    • Fibrinogen / metabolism
    • Fibroblast Growth Factor 2 / genetics
    • Fibroblast Growth Factor 2 / metabolism
    • Gene Expression Profiling
    • Gene Expression Regulation, Developmental
    • Horses / embryology
    • Horses / genetics
    • Horses / metabolism
    • Immune System / embryology
    • Immunohistochemistry
    • Male
    • Maternal-Fetal Exchange
    • N-Acetylneuraminic Acid / metabolism
    • Polymerase Chain Reaction
    • Pregnancy
    • RNA, Messenger / metabolism
    • Trophoblasts / metabolism

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