Transcriptome analyses of bovine, porcine and equine endometrium during the pre-implantation phase.
Abstract: Different reproductive strategies evolved in various mammalian groups to achieve recognition, establishment and maintenance of pregnancy. The complexity of these processes is reflected by a high incidence of embryonic loss during this critical period in many mammalian species. Besides studies in mice and humans a number of transcriptome studies of endometrial tissue samples and also of early embryos have been performed during the pre-implantation phase in cattle, swine and horse to identify genes associated with embryo-maternal interaction. Results of these studies are reviewed and compared between species. The comparison of data sets from different species indicated a general role of interferons for the establishment of pregnancy. In addition to many species-specific changes in gene expression, which may reflect different pregnancy recognition signals and mechanisms of embryo implantation, a number of transcriptome changes were found to be similar across species. These genes may have conserved roles during the establishment of pregnancy in mammals and reflect basic principles of mammalian reproduction. The relevance and strategies, but also the challenges of cross-species comparisons of gene expression data are discussed.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2012-08-11 PubMed ID: 22917876DOI: 10.1016/j.anireprosci.2012.08.015Google Scholar: Lookup
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- Journal Article
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- Non-U.S. Gov't
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Summary
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The research investigates the differences and similarities in genes of the endometrium in cows, pigs, and horses during the pre-implantation phase of pregnancy, aiming to understand the genetic factors behind the success of pregnancy establishment in these species.
Analyses of Bovine, Porcine and Equine Endometrium
The research utilised transcriptome analyses of endometrial tissue samples collected from cows, pigs, and horses during the early stages of pregnancy, more specifically, during the pre-implantation phase. The
- The researchers aimed to identify genes associated with interactions between the embryo and the mother.
- The study performed a review and comparison of results between different mammalian species.
Establishment of Pregnancy
The data from the different species showed general consistency that suggested the essential role of interferons in the establishment of pregnancy.
- Many changes in gene expression were species-specific, possibly indicating different pregnancy recognition signals and mechanisms of embryo implantation.
- However, there were also several changes in the transcriptome that were similar across different species.
- These shared genes likely have conserved roles in establishing pregnancy in mammals, implying the existence of fundamental principles for mammalian reproduction.
Cross-Species Comparisons
The relevance and strategies involved in the cross-species comparison of gene expression data were discussed, as well as the challenges encountered with such an approach.
- This research shed light on how reproductive strategies have evolved over time in various mammal groups.
- It also highlighted the percentage of embryonic loss during this critical period, showing the complexity of the processes that lead to successful pregnancy.
Overall, the study provides valuable insights into the genetic factors that contribute to the success of pregnancy establishment in mammals. Such understanding could inform progress in reproductive technologies and treatments for infertility in both animals and humans.
Cite This Article
APA
Bauersachs S, Wolf E.
(2012).
Transcriptome analyses of bovine, porcine and equine endometrium during the pre-implantation phase.
Anim Reprod Sci, 134(1-2), 84-94.
https://doi.org/10.1016/j.anireprosci.2012.08.015 Publication
Researcher Affiliations
- Molecular Animal Breeding & Biotechnology and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Feodor-Lynen-Str. 25, 81377 Munich, Germany. bsachs@genzentrum.lmu.de
MeSH Terms
- Animals
- Blastocyst / physiology
- Cattle
- Embryonic Development / physiology
- Endometrium / metabolism
- Female
- Gene Expression Regulation / physiology
- Horses
- Pregnancy
- Pregnancy, Animal / physiology
- Swine
- Transcriptome
Citations
This article has been cited 18 times.- Vegas AR, Podico G, Canisso IF, Bollwein H, Fröhlich T, Bauersachs S, Almiñana C. Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy.. FASEB Bioadv 2022 Dec;4(12):775-797.
- Ribeiro ES, Spricigo JFW, Carvalho MR, Ticiani E. Physiological and cellular requirements for successful elongation of the preimplantation conceptus and the implications for fertility in lactating dairy cows.. Anim Reprod 2018 Jul-Sep;15(Suppl 1):765-790.
- Adur MK, Seibert JT, Romoser MR, Bidne KL, Baumgard LH, Keating AF, Ross JW. Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period.. J Anim Sci 2022 Jul 1;100(7).
- Rudolf Vegas A, Podico G, Canisso IF, Bollwein H, Almiñana C, Bauersachs S. Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare.. Sci Rep 2021 Nov 16;11(1):22293.
- Swegen A. Maternal recognition of pregnancy in the mare: does it exist and why do we care?. Reproduction 2021 May 5;161(6):R139-R155.
- Kasimanickam RK, Kasimanickam VR. mRNA Expressions of Candidate Genes in Gestational Day 16 Conceptus and Corresponding Endometrium in Repeat Breeder Dairy Cows with Suboptimal Uterine Environment Following Transfer of Different Quality Day 7 Embryos.. Animals (Basel) 2021 Apr 11;11(4).
- Buska-Mach K, Kedzierska AE, Lepczynski A, Herosimczyk A, Ozgo M, Karpinski P, Gomulkiewicz A, Lorek D, Slawek A, Dziegiel P, Chelmonska-Soyta A. Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice.. Front Vet Sci 2021;8:641553.
- Gibson C, de Ruijter-Villani M, Bauersachs S, Stout TAE. Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy.. Int J Mol Sci 2020 Apr 7;21(7).
- Eozenou C, Lesage-Padilla A, Mauffré V, Healey GD, Camous S, Bolifraud P, Giraud-Delville C, Vaiman D, Shimizu T, Miyamoto A, Sheldon IM, Constant F, Pannetier M, Sandra O. FOXL2 is a Progesterone Target Gene in the Endometrium of Ruminants.. Int J Mol Sci 2020 Feb 21;21(4).
- Smits K, Gansemans Y, Tilleman L, Van Nieuwerburgh F, Van De Velde M, Gerits I, Ververs C, Roels K, Govaere J, Peelman L, Deforce D, Van Soom A. Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers?. Int J Mol Sci 2020 Jan 9;21(2).
- Zeng S, Ulbrich SE, Bauersachs S. Spatial organization of endometrial gene expression at the onset of embryo attachment in pigs.. BMC Genomics 2019 Nov 21;20(1):895.
- Moraes JGN, Behura SK, Geary TW, Spencer TE. Analysis of the uterine lumen in fertility-classified heifers: I. Glucose, prostaglandins, and lipids†.. Biol Reprod 2020 Feb 14;102(2):456-474.
- Moraes JGN, Behura SK, Bishop JV, Hansen TR, Geary TW, Spencer TE. Analysis of the uterine lumen in fertility-classified heifers: II. Proteins and metabolites†.. Biol Reprod 2020 Mar 13;102(3):571-587.
- Smits K, Willems S, Van Steendam K, Van De Velde M, De Lange V, Ververs C, Roels K, Govaere J, Van Nieuwerburgh F, Peelman L, Deforce D, Van Soom A. Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse.. Sci Rep 2018 Mar 27;8(1):5249.
- Soleilhavoup C, Riou C, Tsikis G, Labas V, Harichaux G, Kohnke P, Reynaud K, de Graaf SP, Gerard N, Druart X. Proteomes of the Female Genital Tract During the Oestrous Cycle.. Mol Cell Proteomics 2016 Jan;15(1):93-108.
- Córdoba S, Balcells I, Castelló A, Ovilo C, Noguera JL, Timoneda O, Sánchez A. Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency.. Sci Rep 2015 Oct 5;5:14416.
- Schroyen M, Tuggle CK. Current transcriptomics in pig immunity research.. Mamm Genome 2015 Feb;26(1-2):1-20.
- Wolf E, Braun-Reichhart C, Streckel E, Renner S. Genetically engineered pig models for diabetes research.. Transgenic Res 2014 Feb;23(1):27-38.
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