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Home / Equine Research Bank / Genes (Basel) / Transcriptome Analysis Reveals Equine Endometrium’s Ge...
Genes2025; 16(2); doi: 10.3390/genes16020181

Transcriptome Analysis Reveals Equine Endometrium’s Gene Expression Profile Around Embryo Fixation.

Abstract: The success or failure of embryo fixation is crucial for embryo attachment and later development. As an epithelial chorioallantoic placenta-type animal, the horse has a special process of embryo implantation, and the mechanism of embryo fixation in horses is still unclear. Methods: In this study, the structural and transcriptomic characteristics of endometrial tissue from the fixed and nonfixed sides of 20-day gestation embryos in Mongolian horses were investigated to search for important genes and potential molecular markers associated with the fixation phase of equine embryos. Results: A comparison of the structures of the endometrial tissues of the two sides revealed that the endometrium on the fixed side presented distinctive features, which were characterized mainly by the development of glands on the fixed side compared with those on the nonfixed side. A total of 3987 differentially expressed genes were identified in the transcriptome, among which 1931 genes were highly expressed on the fixed side of the embryo, including CDH1, DRA, DQB, CLND2, BOLA-DQB, CLDN10, PTGER2, and PTGFR. The differentially expressed genes were enriched in biological processes such as cell adhesion, morphogenesis, NOD signaling, and vitamin uptake, as well as prostatic hormones. Conclusions: These results suggest that equine embryo fixation may depend at least on the regulation of prostaglandins and the establishment of cellular connections. This provides a foundation for exploring the molecular mechanisms of key genes and pathways related to equine embryo fixation and offers new insights into feeding management and the monitoring of mares in the early stages of pregnancy.
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Publication Date: 2025-02-01 PubMed ID: 40004510PubMed Central: PMC11855126DOI: 10.3390/genes16020181Google Scholar: Lookup
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  • Journal Article

Summary

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The study explores the molecular mechanisms involved in the fixation phase of equine embryos using a transcriptomic analysis of the endometrial tissue from horses at 20-day gestation.

Study Overview

The research analyses the transcriptomic characteristics of endometrial tissue from the fixed and nonfixed sides of 20-day gestation embryos in Mongolian horses. The objective was to identify key genes and potential molecular markers associated with the fixation phase of equine embryos.

Methods

  • The researchers investigated the structural differences in endometrial tissues on both sides. They observed that the endometrium on the fixed side exhibited unique features, characterized mainly by the development of glands compared to the nonfixed side.
  • They conducted a transcriptome analysis, a technique that allows the study of the complete set of RNA transcripts produced by the genome, to identify differentially expressed genes in the endometrial tissues.

Results and Findings

  • The transcriptome analysis revealed 3987 differentially expressed genes, with 1931 genes being highly expressed on the fixed side of the embryo.
  • The identified differentially expressed genes were found to be involved in biological processes like cell adhesion, morphogenesis, NOD signaling, vitamin uptake, and prostatic hormones.
  • The analysis suggested that these genes might play key roles in the fixation phase of equine embryos. Particularly, the regulation of prostaglandins and the establishment of cellular connections appear to be significant factors.

Conclusions and Implications

  • The findings provide a foundation for further investigation into the molecular mechanisms of key genes and pathways related to equine embryo fixation.
  • This study provides new insights that could potentially improve feeding management and the monitoring of mares in the early stages of pregnancy.

Cite This Article

APA
Ulaangerel T, Mu S, Sodyelalt J, Yi M, Zhao B, Hao A, Wen X, Han B, Bou G. (2025). Transcriptome Analysis Reveals Equine Endometrium’s Gene Expression Profile Around Embryo Fixation. Genes (Basel), 16(2). https://doi.org/10.3390/genes16020181

Publication

Genes
ISSN: 2073-4425
NlmUniqueID: 101551097
Country: Switzerland
Language: English
Volume: 16
Issue: 2

Researcher Affiliations

Ulaangerel, Tseweendolmaa
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Mu, Siqin
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Sodyelalt, Jolanqiqige
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Yi, Minna
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhao, Bilig
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Hao, Asiya
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wen, Xin
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Han, Baoxiang
  • College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bou, Gerelchimeg
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

MeSH Terms

  • Animals
  • Horses / genetics
  • Horses / embryology
  • Female
  • Endometrium / metabolism
  • Transcriptome / genetics
  • Gene Expression Profiling
  • Pregnancy
  • Gene Expression Regulation, Developmental
  • Embryo Implantation / genetics
  • Embryo, Mammalian / metabolism

Grant Funding

  • （ 2023QN03057） / Inner Mongolia Natural Sciences Fund

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 42 references
  1. Navarro S, Giraudo P, Karseladze AI, Smirnov A, Petrovichev N, Savelov N, Alvarado-Cabrero I, Llombart-Bosch A. Immunophenotypic profile of biomarkers related to anti-apoptotic and neural development pathways in the Ewing’s family of tumors (EFT) and their therapeutic implications.. Anticancer Res. 2007;27:2457–2463.
    pubmed: 17695539
  2. Allen WR, Wilsher S. A review of implantation and early placentation in the mare.. Placenta 2009;30:1005–1015.
    doi: 10.1016/j.placenta.2009.09.007pubmed: 19850339google scholar: lookup
  3. Gastal MO, Gastal EL, Kot K, Ginther OJ. Factors related to the time of fixation of the conceptus in mares.. Theriogenology 1996;46:1171–1180.
    doi: 10.1016/S0093-691X(96)00288-9pubmed: 16727980google scholar: lookup
  4. Cross DT, Ginther OJ. Uterine contractions in nonpregnant and early pregnant mares and jennies as determined by ultrasonography.. J. Anim. Sci. 1988;66:250–254.
    doi: 10.2527/jas1988.661250xpubmed: 3284864google scholar: lookup
  5. Carnevale EM, Ginther OJ. Relationships of age to uterine function and reproductive efficiency in mares.. Theriogenology 1992;37:1101–1115.
    doi: 10.1016/0093-691X(92)90108-4pubmed: 16727108google scholar: lookup
  6. Griffin PG, Ginther OJ. Uterine contractile activity in mares during the estrous cycle and early pregnancy.. Theriogenology 1990;34:47–56.
    doi: 10.1016/0093-691X(90)90576-Fpubmed: 16726815google scholar: lookup
  7. Dangudubiyyam SV, Ginther OJ. Relationship between more follicles in right than left ovary in recently born calves and right ovary propensity for ovulation in cattle.. Reprod. Biol. 2019;19:363–367.
    doi: 10.1016/j.repbio.2019.09.005pubmed: 31563451google scholar: lookup
  8. Vergaro P, Tiscornia G, Rodriguez A, Santalo J, Vassena R. Transcriptomic analysis of the interaction of choriocarcinoma spheroids with receptive vs. non-receptive endometrial epithelium cell lines: An in vitro model for human implantation.. J. Assist. Reprod. Genet. 2019;36:857–873.
    doi: 10.1007/s10815-019-01442-9pmc: PMC6541682pubmed: 30972518google scholar: lookup
  9. Sharkey AM, Macklon NS. The science of implantation emerges blinking into the light.. Reprod. Biomed. Online. 2013;27:453–460.
    doi: 10.1016/j.rbmo.2013.08.005pubmed: 24055396google scholar: lookup
  10. Hannan NJ, Jones RL, White CA, Salamonsen LA. The chemokines, CX3CL1, CCL14, and CCL4, promote human trophoblast migration at the feto-maternal interface.. Biol. Reprod. 2006;74:896–904.
    doi: 10.1095/biolreprod.105.045518pubmed: 16452465google scholar: lookup
  11. Hannan NJ, Paiva P, Meehan KL, Rombauts LJ, Gardner DK, Salamonsen LA. Analysis of fertility-related soluble mediators in human uterine fluid identifies VEGF as a key regulator of embryo implantation.. Endocrinology 2011;152:4948–4956.
    doi: 10.1210/en.2011-1248pubmed: 22028446google scholar: lookup
  12. Greening DW, Nguyen HP, Elgass K, Simpson RJ, Salamonsen LA. Human Endometrial Exosomes Contain Hormone-Specific Cargo Modulating Trophoblast Adhesive Capacity: Insights into Endometrial-Embryo Interactions.. Biol. Reprod. 2016;94:38.
    doi: 10.1095/biolreprod.115.134890pubmed: 26764347google scholar: lookup
  13. Yang Y, Chen X, Saravelos SH, Liu Y, Huang J, Zhang J, Li TC. HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage.. Fertil. Steril. 2017;107:136–143.e2.
    doi: 10.1016/j.fertnstert.2016.09.016pubmed: 27793380google scholar: lookup
  14. Floridon C, Nielsen O, Holund B, Sunde L, Westergaard JG, Thomsen SG, Teisner B. Localization of E-cadherin in villous, extravillous and vascular trophoblasts during intrauterine, ectopic and molar pregnancy.. Mol. Hum. Reprod. 2000;6:943–950.
    doi: 10.1093/molehr/6.10.943pubmed: 11006324google scholar: lookup
  15. Blechschmidt K, Mylonas I, Mayr D, Schiessl B, Schulze S, Becker KF, Jeschke U. Expression of E-cadherin and its repressor snail in placental tissue of normal, preeclamptic and HELLP pregnancies.. Virchows Arch. 2007;450:195–202.
    doi: 10.1007/s00428-006-0343-xpubmed: 17149611google scholar: lookup
  16. Nollet F, Kools P, van Roy F. Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.. J. Mol. Biol. 2000;299:551–572.
    doi: 10.1006/jmbi.2000.3777pubmed: 10835267google scholar: lookup
  17. Paria BC, Zhao X, Das SK, Dey SK, Yoshinaga K. Zonula occludens-1 and E-cadherin are coordinately expressed in the mouse uterus with the initiation of implantation and decidualization.. Dev. Biol. 1999;208:488–501.
    doi: 10.1006/dbio.1999.9206pubmed: 10191061google scholar: lookup
  18. Jha RK, Titus S, Saxena D, Kumar PG, Laloraya M. Profiling of E-cadherin, β-catenin and Ca(2+) in embryo–uterine interactions at implantation.. FEBS Lett. 2006;580:5653–5660.
    doi: 10.1016/j.febslet.2006.09.014pubmed: 17011554google scholar: lookup
  19. Riethmacher D, Brinkmann V, Birchmeier C. A targeted mutation in the mouse E-cadherin gene results in defective preimplantation development.. Proc. Natl. Acad. Sci. USA. 1995;92:855–859.
    doi: 10.1073/pnas.92.3.855pmc: PMC42719pubmed: 7846066google scholar: lookup
  20. Coutifaris C, Kao LC, Sehdev HM, Chin U, Babalola GO, Blaschuk OW, Strauss JF. E-cadherin expression during the differentiation of human trophoblasts.. Development 1991;113:767–777.
    doi: 10.1242/dev.113.3.767pubmed: 1821848google scholar: lookup
  21. van Roy F, Berx G. The cell-cell adhesion molecule E-cadherin.. Cell Mol. Life Sci. 2008;65:3756–3788.
    doi: 10.1007/s00018-008-8281-1pmc: PMC11131785pubmed: 18726070google scholar: lookup
  22. Jiang WG, Mansel RE. E-cadherin complex and its abnormalities in human breast cancer.. Surg. Oncol. 2000;9:151–171.
    doi: 10.1016/S0960-7404(01)00010-Xpubmed: 11476987google scholar: lookup
  23. Shirane A, Wada-Hiraike O, Tanikawa M, Seiki T, Hiraike H, Miyamoto Y, Sone K, Hirano M, Oishi H, Oda K. Regulation of SIRT1 determines initial step of endometrial receptivity by controlling E-cadherin expression.. Biochem. Biophys. Res. Commun. 2012;424:604–610.
    doi: 10.1016/j.bbrc.2012.06.160pubmed: 22780949google scholar: lookup
  24. Ginther OJ. Equine embryo mobility. A game changer.. Theriogenology 2021;174:131–138.
    doi: 10.1016/j.theriogenology.2021.08.006pubmed: 34450564google scholar: lookup
  25. Boerboom D, Brown KA, Vaillancourt D, Poitras P, Goff AK, Watanabe K, Doré M, Sirois J. Expression of key prostaglandin synthases in equine endometrium during late diestrus and early pregnancy.. Biol. Reprod. 2004;70:391–399.
    doi: 10.1095/biolreprod.103.020800pubmed: 14561653google scholar: lookup
  26. Hyland JH, Manns JG, Humphrey WD. Prostaglandin production by ovine embryos and endometrium in vitro.. J. Reprod. Fertil. 1982;65:299–304.
    doi: 10.1530/jrf.0.0650299pubmed: 7097639google scholar: lookup
  27. Hwang DH, Pool SH, Rorie RW, Boudreau M, Godke RA. Transitional changes in arachidonic acid metabolism by bovine embryos at different developmental stages.. Prostaglandins 1988;35:387–402.
    doi: 10.1016/0090-6980(88)90130-Xpubmed: 3131836google scholar: lookup
  28. Watson ED, Sertich PL. Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares.. J. Reprod. Fertil. 1989;87:331–336.
    doi: 10.1530/jrf.0.0870331pubmed: 2621704google scholar: lookup
  29. Maitre JL, Heisenberg CP. Three functions of cadherins in cell adhesion.. Curr. Biol. 2013;23:R626–R633.
    doi: 10.1016/j.cub.2013.06.019pmc: PMC3722483pubmed: 23885883google scholar: lookup
  30. McCracken JA, Custer EE, Lamsa JC. Luteolysis: A neuroendocrine-mediated event.. Physiol. Rev. 1999;79:263–323.
    doi: 10.1152/physrev.1999.79.2.263pubmed: 10221982google scholar: lookup
  31. Davis JS, Rueda BR. The corpus luteum: An ovarian structure with maternal instincts and suicidal tendencies.. Front. Biosci. 2002;7:d1949–d1978.
    doi: 10.2741/davis1pubmed: 12161347google scholar: lookup
  32. Sirois J, Dore M. The late induction of prostaglandin G/H synthase-2 in equine preovulatory follicles supports its role as a determinant of the ovulatory process.. Endocrinology 1997;138:4427–4434.
    doi: 10.1210/endo.138.10.5462pubmed: 9322960google scholar: lookup
  33. Kargman S, Charleson S, Cartwright M, Frank J, Riendeau D, Mancini J, Evans J, O’Neill G. Characterization of Prostaglandin G/H Synthase 1 and 2 in rat, dog, monkey, and human gastrointestinal tracts.. Gastroenterology 1996;111:445–454.
    doi: 10.1053/gast.1996.v111.pm8690211pubmed: 8690211google scholar: lookup
  34. Stout TA, Allen WR. Prostaglandin E0 and F2α production by equine conceptuses and concentrations in conceptus fluids and uterine flushings recovered from early pregnant and dioestrous mares.. Reproduction 2002;123:261–268.
    doi: 10.1530/rep.0.1230261pubmed: 11866693google scholar: lookup
  35. Piotrowska-Tomala KK, Jonczyk AW, Skarzynski DJ, Szostek-Mioduchowska AZ. Luteinizing hormone and ovarian steroids affect in vitro prostaglandin production in the equine myometrium and endometrium.. Theriogenology 2020;153:1–8.
    doi: 10.1016/j.theriogenology.2020.04.039pubmed: 32416544google scholar: lookup
  36. Budik S, Walter I, Leitner MC, Ertl R, Aurich C. Expression of Enzymes Associated with Prostaglandin Synthesis in Equine Conceptuses.. Animals 2021;11:1180.
    doi: 10.3390/ani11041180pmc: PMC8074782pubmed: 33924239google scholar: lookup
  37. Weber JA, Freeman DA, Vanderwall DK, Woods GL. Prostaglandin E2 secretion by oviductal transport-stage equine embryos.. Biol. Reprod. 1991;45:540–543.
    doi: 10.1095/biolreprod45.4.540pubmed: 1751627google scholar: lookup
  38. Weber JA, Freeman DA, Vanderwall DK, Woods GL. Prostaglandin E2 hastens oviductal transport of equine embryos.. Biol. Reprod. 1991;45:544–546.
    doi: 10.1095/biolreprod45.4.544pubmed: 1751628google scholar: lookup
  39. Stout TA. Embryo–maternal communication during the first 4 weeks of equine pregnancy.. Theriogenology 2016;86:349–354.
    doi: 10.1016/j.theriogenology.2016.04.048pubmed: 27156682google scholar: lookup
  40. Klein C. Early pregnancy in the mare: Old concepts revisited.. Domest. Anim. Endocrinol. 2016;56:212–217.
    doi: 10.1016/j.domaniend.2016.03.006pubmed: 27345319google scholar: lookup
  41. Allen WR. Fetomaternal interactions and influences during equine pregnancy.. Reproduction 2001;121:513–527.
    doi: 10.1530/rep.0.1210513pubmed: 11277870google scholar: lookup
  42. Goff AK, Pontbriand D, Sirois J. Oxytocin stimulation of plasma 15-keto-13,14-dihydro prostaglandin F-2 alpha during the oestrous cycle and early pregnancy in the mare.. J. Reprod. Fertil. Suppl. 1987;35:253–260.
    pubmed: 3479581
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