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Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts.
Abstract: The objective of our study was to evaluate the integration of autologous cartilage tissue engineering transplants based on resorbable polyglactin/polydioxanone scaffolds into full-thickness cartilage defects of horses. Cartilage biopsies were taken from the non-load-bearing area of the lateral talus of the left tibiotarsal joint of eight healthy Haflinger horses. Tissue engineering cartilage transplants were generated by three-dimensional arrangement of autologous chondrocytes in biocompatible and resorbable polymer scaffolds. Full-thickness cartilage defects of 8 mm in diameter were created in the tubular bone condyle of the fetlock joint and cartilage grafts were fixed using an anchor system, while defects without grafting served as controls. After 6 and 12 months the repair tissue was evaluated histologically and showed formation of a cartilaginous tissue and good integration into the surrounding host tissue with firm bonding of the graft to the adjacent cartilage and the underlying subchondral bone. Biochemical analysis demonstrated that the content of glycosaminoglycans and hydroxyproline is comparable in repair tissue derived from treated and control defects. The use of three-dimensional autologous cartilage transplants based on resorbable polymer scaffolds ensures secure fixation, good integration of the graft into cartilage lesions, and is therefore suggested as a promising therapeutic option for the treatment of cartilage defects.
Publication Date: 2006-01-25 PubMed ID: 16442157DOI: 10.1016/j.biomaterials.2006.01.008Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research explores the use of a particular type of resorbable polymer scaffold in treating full-thickness cartilage defects in horses. Results showed that autologous cartilage tissue engineering transplants, created using the polymer scaffolds, were successfully integrated into the horse’s cartilage injuries.
Objective of the Study
- The main aim of this research was to investigate the effectiveness of resorbable polyglactin/polydioxanone scaffolds in treating full-thickness cartilage defects in horses. This is considered by creating three-dimensional autologous cartilage transplants using these biocompatible and resorbable polymer scaffolds.
Research Methodology
- Cartilage biopsies were taken from the non-load-bearing area of eight healthy horses.
- The researched then created the tissue engineering cartilage transplants by arranging the autologous chondrocytes in three-dimensional form using polyglactin/polydioxanone scaffolds. These are biocompatible and resorbable polymer scaffolds.
- The team created full-thickness cartilage defects in the fetlock joint’s tubular bone condyle. They then fixed the cartilage grafts using an anchor system. They left some defects without grafting to serve as control.
Results and Findings
- The findings were evaluated after 6 and 12 months. The study found the formation of cartilaginous tissue and good integration into the surrounding host tissue. There was firm bonding of the graft to the adjacent cartilage and the underlying subchondral bone.
- The biochemical analysis showed that the content of glycosaminoglycans and hydroxyproline in both the treated and control defects were similar. This suggested that the transplant did not adversely affect the biochemical composition of the cartilage repair tissue.
Conclusion
- The use of resorbable polymer scaffolds to create three-dimensional autologous cartilage transplants provided secure fixation, and good integration of the graft into the cartilage lesions of the horses.
- This puts forward a promising therapeutic option for treating full-thickness cartilage defects in horses. It could potentially be applied in other animals or even human cartilage injury treatment in the future.
Cite This Article
APA
Barnewitz D, Endres M, Krüger I, Becker A, Zimmermann J, Wilke I, Ringe J, Sittinger M, Kaps C.
(2006).
Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts.
Biomaterials, 27(14), 2882-2889.
https://doi.org/10.1016/j.biomaterials.2006.01.008 Publication
Researcher Affiliations
- Research Center of Medical Technology and Biotechnology, Bad Langensalza, Germany.
MeSH Terms
- Animals
- Biocompatible Materials
- Cartilage, Articular / pathology
- Chromatography, High Pressure Liquid
- Female
- Horses
- Magnetic Resonance Imaging
- Male
- Polymers
- Tissue Engineering
Citations
This article has been cited 15 times.- Carvalho JRG, Conde G, Antonioli ML, Santana CH, Littiere TO, Dias PP, Chinelatto MA, Canola PA, Zara FJ, Ferraz GC. Long-Term Evaluation of Poly(lactic acid) (PLA) Implants in a Horse: An Experimental Pilot Study. Molecules 2021 Nov 29;26(23).
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- Ruediger T, Horbert V, Reuther A, Kumar Kalla P, Burgkart RH, Walther M, Kinne RW, Mika J. Thickness of the Stifle Joint Articular Cartilage in Different Large Animal Models of Cartilage Repair and Regeneration. Cartilage 2021 Dec;13(2_suppl):438S-452S.
- Huang BJ, Hu JC, Athanasiou KA. Cell-based tissue engineering strategies used in the clinical repair of articular cartilage. Biomaterials 2016 Aug;98:1-22.
- Hurtig MB, Buschmann MD, Fortier LA, Hoemann CD, Hunziker EB, Jurvelin JS, Mainil-Varlet P, McIlwraith CW, Sah RL, Whiteside RA. Preclinical Studies for Cartilage Repair: Recommendations from the International Cartilage Repair Society. Cartilage 2011 Apr;2(2):137-52.
- He X, Feng B, Huang C, Wang H, Ge Y, Hu R, Yin M, Xu Z, Wang W, Fu W, Zheng J. Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering. Int J Nanomedicine 2015;10:2089-99.
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- Batista NA, Rodrigues AA, Bavaresco VP, Mariolani JR, Belangero WD. Polyvinyl alcohol hydrogel irradiated and acetalized for osteochondral defect repair: mechanical, chemical, and histological evaluation after implantation in rat knees. Int J Biomater 2012;2012:582685.
- Endres M, Neumann K, Zhou B, Freymann U, Pretzel D, Stoffel M, Kinne RW, Kaps C. An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts. J Orthop Surg Res 2012 Nov 9;7:37.
- Dehne T, Zehbe R, Krüger JP, Petrova A, Valbuena R, Sittinger M, Schubert H, Ringe J. A method to screen and evaluate tissue adhesives for joint repair applications. BMC Musculoskelet Disord 2012 Sep 17;13:175.
- Kreuz PC, Müller S, Ossendorf C, Kaps C, Erggelet C. Treatment of focal degenerative cartilage defects with polymer-based autologous chondrocyte grafts: four-year clinical results. Arthritis Res Ther 2009;11(2):R33.
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- Ossendorf C, Kaps C, Kreuz PC, Burmester GR, Sittinger M, Erggelet C. Treatment of posttraumatic and focal osteoarthritic cartilage defects of the knee with autologous polymer-based three-dimensional chondrocyte grafts: 2-year clinical results. Arthritis Res Ther 2007;9(2):R41.
- Emonts C, Bauer B, Büchter C, Pufe T, Gries T, Tohidnezhad M. Effect of Collagen Coating and Fiber Profile on Tenocyte Growth on Braided Poly-ε-Caprolactone Scaffolds for Tendon and Ligament Regeneration. Int J Mol Sci 2025 Feb 18;26(4).
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