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Frontiers in veterinary science2024; 11; 1376578; doi: 10.3389/fvets.2024.1376578

Treatment of experimental hyperchloremic metabolic acidosis in horses with enteral electrolyte solution containing sodium acetate.

Abstract: In adult horses, the development and evaluation of enteral electrolyte solutions containing sodium acetate for correcting hyperchloremic metabolic acidosis are still lacking, although these electrolyte and acid-base imbalances are commonly observed. The objective of this study was to evaluate the alkalinizing effect of two enteral electrolyte solutions containing different concentrations of acetate, administered via nasogastric tube in continuous flow, in adult horses with experimental hyperchloremic metabolic acidosis. Unassigned: Six mares aged between 3 and 10 years were used in a 6×2 crossover design, with each animal receiving both treatments. The horses were subjected to a protocol to induce hyperchloremic metabolic acidosis. They then received one of two treatments: HighAcetate (81.4 mmol/L) and LowAcetate (22.7 mmol/L) at an infusion rate of 15 mL/kg/h for 12 h. Plasma, serum, and urinary biochemical assessments; hematocrit; urinary volume, pH, and specific gravity; and blood gas analysis were measured at the following time points: T-12 h (beginning of the 12-h fast), T0h (end of fasting and beginning of the acidosis induction phase), every 2 h during the hyperchloremic metabolic acidosis induction phase (T2h and T4h), every 2 h during the 12-h enteral hydration phase (T2h, T4h, Tt6h, T8h, T10h, and T12h), with one sample taken at T24h (24 h after the start of acidosis induction) and another at T36h (36 h after the start of acidosis induction). Data were analyzed using descriptive statistics and analysis of variance based on a factorial design of repeated measures, with Tukey's test or the Kruskal-Wallis test with Dunn's test for non-parametric tests. Unassigned: At the end of the induction phase, the animals developed moderate to severe hyperchloremic metabolic acidosis. The HighAcetate solution effectively corrected electrolyte and acid-base imbalances before the end of the treatment phase (Tt12h), while the LowAcetate solution was not effective in correcting those changes. Unassigned: The HighAcetate (81.4 mmol/L) solution is deemed an effective and safe alternative for the treatment of hyperchloremic metabolic acidosis in horses.
Copyright © 2024 Monteiro, Costa, Ermita, Júnior, Mattos, Mansur, dos Santos, Alves, Mafort, Fidélis, Avanza, Teixeira, Viana and Filho.
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Publication Date: 2024-09-11 PubMed ID: 39323875PubMed Central: PMC11422386DOI: 10.3389/fvets.2024.1376578Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is about the treatment of hyperchloremic metabolic acidosis in horses using sodium acetate. The study suggests that using a high concentration of Sodium Acetate is an effective treatment for such condition in horses.

Introduction and Objective of the Study

  • The research focuses on the evaluation of sodium acetate in enteral electrolyte solutions for its ability to correct hyperchloremic metabolic acidosis in horses. Hyperchloremic metabolic acidosis is a common acid-base imbalance in horses which hasn’t been adequately addressed.
  • The researchers have experimented with two electrolyte solutions containing different concentrations of acetate in an attempt to discern their alkalinizing effect in the course of the disorder. The solutions were administered to adult horses suffering from the induced disorder through nasogastric tubes, in continuous flow.

Methodology

  • Six mares aged from 3 to 10 years were used in the study. The sample selection followed a 6×2 crossover design, which allowed each horse to receive both treatments for a comprehensive evaluation.
  • The horses underwent a protocol to induce hyperchloremic metabolic acidosis. Post-induction, they received one of two treatments: HighAcetate (81.4 mmol/L) and LowAcetate (22.7 mmol/L). The infusion rate was set to 15 ml/kg/hr for a period of 12 hours.
  • Several measurements and assessments were conducted pre and post-infusion, including plasma, serum, and urinary biochemical assessments, hematocrit, urinary volume, pH, specific gravity, and blood gas analysis.

Data Analysis

  • For analyzing the collected data, they employed descriptive statistics and analysis of variance based on a factorial design of repeated measures, accompanied by Tukey’s test or the Kruskal-Wallis test with Dunn’s test for non-parametric tests.

Results

  • The research showed that, post the induction phase, the horses developed moderate to severe hyperchloremic metabolic acidosis.
  • It was observed that the HighAcetate solution was effective in correcting the electrolyte and acid-base imbalances before the end of the treatment phase. In contrast, the LowAcetate solution didn’t show effective results in counteracting the changes.

Conclusion

  • The study concluded that the HighAcetate solution containing 81.4 mmol/L of acetate is an efficient and safe alternative for treating hyperchloremic metabolic acidosis in horses.

Cite This Article

APA
Monteiro LC, Costa CM, Ermita PAN, Júnior SJPG, Mattos FS, Mansur FC, Dos Santos MO, Alves SR, Mafort EG, Fidélis CF, Avanza MFB, Teixeira RBC, Viana RB, Filho JDR. (2024). Treatment of experimental hyperchloremic metabolic acidosis in horses with enteral electrolyte solution containing sodium acetate. Front Vet Sci, 11, 1376578. https://doi.org/10.3389/fvets.2024.1376578

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 11
Pages: 1376578
PII: 1376578

Researcher Affiliations

Monteiro, Lorena Chaves
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Costa, Caio Monteiro
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Ermita, Pedro Ancelmo Nunes
  • Universidade Federal do Sul e Sudeste do Pará, Xinguara, Brazil.
Júnior, Silvio José Printes Gomes
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Mattos, Felipe Sperandio
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Mansur, Fernanda Campos
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Dos Santos, Mayara Oliveira
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Alves, Samuel Rodrigues
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Mafort, Erica Garcia
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Fidélis, Cíntia Fernandes
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Avanza, Marcel Ferreira Bastos
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Teixeira, Raffaella Bertoni Cavalvanti
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.
Viana, Rinaldo Batista
  • Institute of Animal Health and Production, Universidade Federal Rural da Amazônia, Belém, Brazil.
Filho, José Dantas Ribeiro
  • Laborary of Research in Veterinary Internal Medicine, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Brazil.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

This article includes 49 references
  1. Navarro M, Monreal L, Segura D, Armengou L, Añor S. A comparison of traditional and quantitative analysis of Acid-Base and electrolyte imbalances in horses with gastrointestinal disorders.. J Vet Intern Med (2005) 19:871–7.
    doi: 10.1111/j.1939-1676.2005.tb02780.xpubmed: 16355683google scholar: lookup
  2. Gomez DE, Arroyo LG, Stämpfli HR, Cruz LE, Oliver OJ. Physicochemical interpretation of Acid-Base abnormalities in 54 adult horses with acute severe colitis and diarrhea.. J Vet Intern Med (2013) 27:548–53.
    doi: 10.1111/jvim.12071pubmed: 23551698google scholar: lookup
  3. Ecke P, Hodgson DR, Rose RJ. Induced diarrhoea in horses part 2: response toadministration of an oral rehydration solution.. Vet J (1998) 155:161–70.
    doi: 10.1016/S1090-0233(98)80012-9pubmed: 9564269google scholar: lookup
  4. Di Filippo PA, Santana AE, Pereira GT. Equilíbrio ácido-base e hidroeletrolítico em eqüinos com cólica.. Ciência Rural (2008) 38:1003–9.
    doi: 10.1590/S0103-84782008000400015google scholar: lookup
  5. Schott HC. Chronic renal failure in horses.. Vet Clin N Am Equine Pract (2007) 23:593–612.
    doi: 10.1016/j.cveq.2007.10.002pubmed: 18061852google scholar: lookup
  6. Gomes CLN, Alves AM, Ribeiro Filho JD. Exercise training session-induced metabolic acidosis in barrel racing horses.. Ciência Rural (2020) 50:1–9.
    doi: 10.1590/0103-8478cr20180887google scholar: lookup
  7. Gomez DE, Biermann NM, Sanchez LC. Physicochemical approach to determine the mechanism for Acid-Base disorders in 793 hospitalized foals.. J Vet Intern Med (2015) 29:1395–402.
    doi: 10.1111/jvim.13590pmc: PMC4858039pubmed: 26256847google scholar: lookup
  8. Patra FC, Mahalanabis D, Jalan KN, Sen A, Banerjee P. Can acetate replace bicarbonate in oral rehydration solution for infantile diarrhoea?. Arch Dis Child (1982) 57:625–7.
    doi: 10.1136/adc.57.8.625pmc: PMC1627737pubmed: 7051986google scholar: lookup
  9. Marshall TS, Constable PD, Crochik SS, Wittek T, Freeman DE, Morin DE. Effect of suckling an isotonic solution of sodium acetate, sodium bicarbonate, or sodium chloride on abomasal emptying rate and luminal pH in calves.. Am J Vet Res (2008) 69:824–31.
    doi: 10.2460/ajvr.69.6.824pubmed: 18518665google scholar: lookup
  10. Lopes M. Physiological aspects, indications and contraindications of enteral fluid therapy.. Equine Vet Educ (2002) 14:257–62.
    doi: 10.1111/j.2042-3292.2002.tb00184.xgoogle scholar: lookup
  11. Schumann WC, Magnusson I, Chandramouli V, Kumaran K, Wahren J, Landau BR. Metabolism of [2-14C]acetate and its use in assessing hepatic Krebs cycle activity and gluconeogenesis.. J Biol Chem (1991) 266:6985–90.
    doi: 10.1016/s0021-9258(20)89599-4pubmed: 2016310google scholar: lookup
  12. Krebs H. The history of the tricarboxylic acid cycle of the pathways of anaerobic energy metabolism.. Perspect Biol Med (1970) 14:154–72.
    doi: 10.1353/pbm.1970.0001pubmed: 4923349google scholar: lookup
  13. Monteiro LC, Viana RB, Avanza MFB, Ermita PAN, Costa CM, Alves SR. Effects of hypotonic and isotonic enteral electrolyte solutions administered in continuous flow in weaned foals.. Front Vet Sci (2020) 7:1–10.
    doi: 10.3389/fvets.2020.00280pmc: PMC7256653pubmed: 32596264google scholar: lookup
  14. Monteiro LC, Viana RB, Teixeira RBC, Avanza MFB, Ermita PAN, Costa CM. Use of enteral electrolyte solutions with different sodium acetate concentrations in weaned foals: the effects on acid-base balance, blood glucose, lactate, and urine ph.. Ciência Rural (2022) 52:1–8.
    doi: 10.1590/0103-8478cr20210256google scholar: lookup
  15. Constable PD. Clinical assessment of Acid-Base status: comparison of the Henderson-Hasselbalch and strong ion approaches.. Vet Clin Pathol (2000) 29:115–28.
    doi: 10.1111/j.1939-165X.2000.tb00241.xpubmed: 12070822google scholar: lookup
  16. Constable PD. A simplified strong ion model for acid-base equilibria: application to horse plasma.. J Appl Physiol (1997) 83:297–311.
    doi: 10.1152/jappl.1997.83.1.297pubmed: 9216976google scholar: lookup
  17. Romão FTNMA, Pereira PFV, Flaiban KKMC, Dearo ACO, Fernandes TM, Lisbôa JAN. Intravenous administration of a polyionic solution containing 84 mEq/l of lactate resolves experimentally induced hyperchloraemic acidosis in horses.. Equine Vet J (2017) 49:87–93.
    doi: 10.1111/evj.12525pubmed: 26509916google scholar: lookup
  18. Schwedhelm L, Kirchner D, Klaus B, Bachmann L. Experimentally induced hyperchloremic and dl-lactic acidosis in calves: an attempt to study the effects of oral rehydration on acid-base status.. J Dairy Sci (2013) 96:2464–75.
    doi: 10.3168/jds.2012-6077pubmed: 23415528google scholar: lookup
  19. Abeysekara S, Naylor JM, Wassef AWA, Isak U, Zello GA. D-lactic acid-induced neurotoxicity in a calf model.. Am J Physiol Endocrinol Metab (2007) 293:E558–65.
    doi: 10.1152/ajpendo.00063.2007pubmed: 17505055google scholar: lookup
  20. Romão FTNMA, Curti JM, Pereira PFV, Flaiban KKMC, Lisbôa JAN. Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep.. Arq Bras Med Vet Zootec (2019) 71:53–60.
    doi: 10.1590/1678-4162-10106google scholar: lookup
  21. Kamynina E, Staub O. Concerted action of ENaC, Nedd4–2, and Sgk1 in transepithelial Na + transport.. American J Physiol Renal Physiol (2002) 283:F377–87.
    doi: 10.1152/ajprenal.00143.2002pubmed: 12167587google scholar: lookup
  22. Terker AS, Zhang C, McCormick JA, Lazelle RA, Zhang C, Meermeier NP. Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride.. Cell Metab (2015) 21:39–50.
    doi: 10.1016/j.cmet.2014.12.006pmc: PMC4332769pubmed: 25565204google scholar: lookup
  23. Stewart PA. Independent and dependent variables of acid-base control.. Respir Physiol (1978) 33:9–26.
    doi: 10.1016/0034-5687(78)90079-8pubmed: 27857google scholar: lookup
  24. DuBose TD. Hyperkalemic hyperchloremic metabolic acidosis: pathophysiologic insights.. Kidney Int (1997) 51:591–602.
    doi: 10.1038/ki.1997.85pubmed: 9027745google scholar: lookup
  25. Johnson PJ. Electrolyte and Acid-Base disturbances in the horse.. Vet Clin N Am Equine Pract (1995) 11:491–514.
    doi: 10.1016/S0749-0739(17)30312-7pubmed: 8925422google scholar: lookup
  26. Magner PO, Robinson L, Halperin RM, Zettle R, Halperin ML. The plasma potassium concentration in metabolic acidosis: a re-evaluation.. Am J Kidney Dis (1988) 11:220–4.
    doi: 10.1016/S0272-6386(88)80153-7pubmed: 3344745google scholar: lookup
  27. Wiederseiner JM, Muser J, Lutz T, Hulter HN, Krapf R. Acute metabolic acidosis: characterization and diagnosis of the disorder and the plasma potassium response.. J Am Soc Nephrol (2004) 15:1589–96.
    doi: 10.1097/01.ASN.0000125677.06809.37pubmed: 15153570google scholar: lookup
  28. Simpson DP. Control of hydrogen ion homeostasis and renal acidosis.. Medicine (1971) 50:503–41.
    doi: 10.1097/00005792-197111000-00002pubmed: 4942419google scholar: lookup
  29. Alexander RT, Cordat E, Chambrey R, Dimke H, Eladari D. Acidosis and urinary calcium excretion: insights from genetic disorders.. J Am Soc Nephrol (2016) 27:3511–20.
    doi: 10.1681/ASN.2016030305pmc: PMC5118493pubmed: 27468975google scholar: lookup
  30. Kim G-H. Renal mechanisms for Hypercalciuria induced by metabolic acidosis.. Am J Nephrol (2022) 53:839–46.
    doi: 10.1159/000528089pubmed: 36450225google scholar: lookup
  31. Cosenza M, Pereira PFV, Fernandes LI. Efeito da solução de Ringer com lactato sobre os equilíbrios hidroeletrolítico e acidobase de equinos, ovelhas e bezerros sadios.. Ciência Rural (2013) 43:2247–53.
    doi: 10.1590/s0103-84782013005000128google scholar: lookup
  32. Pinto FC, Bregadioli GC, Carvalho LA, Hasuda AL, Dearo ACO, Flaiban KKMC. A velocidade de infusão da solução poli-iônica intravenosa contendo 84mEq/L de lactato determina a intensidade do efeito alcalinizante em equinos.. Arq Bras Med Vet Zootec (2018) 70:37–44.
    doi: 10.1590/1678-4162-9564google scholar: lookup
  33. Adeva-Andany MM, Fernández-Fernández C, Mouriño-Bayolo D, Castro-Quintela E, Domínguez-Montero A. Sodium bicarbonate therapy in patients with metabolic acidosis.. The Scientific World (2014) 2014:1–13.
    pmc: PMC4227445pubmed: 25405229
  34. Forsythe SM, Schmidt GA. Sodium bicarbonate for the treatment of lactic acidosis.. Chest (2000) 117:260–7.
    doi: 10.1378/chest.117.1.260pubmed: 10631227google scholar: lookup
  35. Dias DCR, Ribeiro Filho JD, Viana RB, Bittencourt TCC, Dantas FTDR, Teixeira RBC. Comparative trial of continuous flow enteral and intravenous fluid therapy in horses.. Front Vet Sci (2021) 8:861.
    doi: 10.3389/FVETS.2021.686425/BIBTEXpmc: PMC8377500pubmed: 34422943google scholar: lookup
  36. Kline K, Frey LP, Foreman JH, Lyman JT. Effects of intravenous sodium bicarbonate and sodium acetate on equine acid-base status.. J Equine Vet (2005) 25:349–54.
    doi: 10.1016/j.jevs.2005.07.003google scholar: lookup
  37. Sen I, Altunok V, Ok M, Coskun A, Constable PD. Efficacy of oral rehydration therapy solutions containing sodium bicarbonate or sodium acetate for treatment of calves with naturally acquired diarrhea, moderate dehydration, and strong ion acidosis.. J Am Vet Med Assoc (2009) 234:926–34.
    doi: 10.2460/javma.234.7.926pubmed: 19335244google scholar: lookup
  38. Naylor JM, Forsyth GW. The alkalinizing effects of metabolizable bases in the healthy calf.. Can J Vet Res (1986) 50:509–16.
    pmc: PMC1255256pubmed: 3024796
  39. Pethick DW, Rose RJ, Bryden WL, Gooden JM. Nutrient utilisation by the hindlimb of thoroughbred horses at rest.. Equine Vet J (1993) 25:41–4.
    doi: 10.1111/j.2042-3306.1993.tb02899.xpubmed: 8422883google scholar: lookup
  40. Waller A, Lindinger MI. The effect of oral sodium acetate administration on plasma acetate concentration and acid-base state in horses.. Acta Vet Scand (2007) 49:1–12.
    doi: 10.1186/1751-0147-49-38pmc: PMC2241837pubmed: 18096070google scholar: lookup
  41. Waller AP, Geor RJ, Spriet LL, Heigenhauser GJF, Lindinger MI. Oral acetate supplementation after prolonged moderate intensity exercise enhances early muscle glycogen resynthesis in horses.. Exp Physiol (2009) 94:888–98.
    doi: 10.1113/expphysiol.2009.047068pubmed: 19429643google scholar: lookup
  42. Mohme H, Molnar S, Lenkeit W. Untersuchungen zur 14 C-Ausscheidung nach oraler Verabreichung von 1-14 C-Acetat beim Ferkel in der Neugeborenenphase.. Z Tierphysiol Tierernahr Futtermittelkd (1970) 26:138–46.
    doi: 10.1111/j.1439-0396.1970.tb00134.xpubmed: 5516849google scholar: lookup
  43. Bobulescu IA, Moe OW. Na+/H+ exchangers in renal regulation of acid-base balance.. Semin Nephrol (2006) 26:334–44.
    doi: 10.1016/j.semnephrol.2006.07.001pmc: PMC2878276pubmed: 17071327google scholar: lookup
  44. Constable PD, Grünberg W, Carstensen L. Comparative effects of two oral rehydration solutions on milk clotting, abomasal luminal pH, and abomasal emptying rate in suckling calves.. J Dairy Sci (2009) 92:296–312.
    doi: 10.3168/jds.2008-1462pubmed: 19109288google scholar: lookup
  45. Stämpfli HR, Carlson GP. How to use the routine serum biochemical profile to understand and interpret Acid-Base disorders in the horse.. Proceed Annual Convention of the AAEP (2001) 47:257–61.
  46. Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals sixth edition.. London: Elsevier; (2008). 916.
  47. Toribio RE. Disorders of calcium and phosphate metabolism in horses.. Vet Clin N Am Equine Pract (2011) 27:129–47.
    doi: 10.1016/j.cveq.2010.12.010pubmed: 21392658google scholar: lookup
  48. Blumberg D, Bonetti A, Jacomella V, Capillo S, Truttmann AC, Lüthy CM. Free circulating magnesium and renal magnesium handling during acute metabolic acidosis in humans.. Am J Nephrol (1998) 18:233–6.
    doi: 10.1159/000013342pubmed: 9627040google scholar: lookup
  49. Ribeiro Filho JD, Pessin AE, Atoji K, Souza MV, Gomes CLN, Silva AR. Enteral fluid therapy: biochemical profile of horses treated with hypotonic enteral electrolyte solutions associated with energy sources.. J Equine Vet (2014) 34:759–64.
    doi: 10.1016/j.jevs.2014.01.004google scholar: lookup

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