Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates.
Abstract: Inflammation-induced P-selectin (CD62P) expression on platelets and endothelial cells facilitates interactions among platelets and polymorphonuclear leukocytes (PMN), and can also promote coagulation. The effects of clopidogrel and aspirin (ASA) on equine platelet CD62P expression were investigated. Six horses were treated in a cross-over design with clopidogrel (2mg/kg PO q 24) or ASA (5mg/kg PO q 24h) for 5 days. Platelets collected at 24, 72, 96, 120, and 168 h after the initiation of therapy were stimulated using 0.1 μg/mL thrombin, followed by flow cytometric analysis using anti-CD41/61 and anti-equine CD62P antibodies. Platelet-PMN aggregates were also enumerated. Baseline CD62P positive platelet numbers were not different between groups (mean ± SD): 4254 ± 1785 (clopidogrel) and 3600 ± 1780 (ASA, P=0. 435). Although expression tended to decrease, there were no significant changes in CD62P+platelets after treatment with either drug (clopidogrel P=0.139, ASA P=0.161). There was also no difference in platelet-PMN aggregates during or after treatment with ASA (P=0.513) or clopidogrel (P=0.543). Due to small numbers of horses, this study may have been underpowered to detect a true decrease in expression, and differences between therapies may have been more pronounced if this study had evaluated horses with systemic inflammation.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2012-06-07 PubMed ID: 22727736DOI: 10.1016/j.vetimm.2012.05.022Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study assessed the impact of aspirin and clopidogrel on P-selectin expression in horse platelets and on the creation of platelet-neutrophil aggregates, finding that neither drug significantly altered these aspects.
Study Design
- Researchers conducted this study using a cross-over design with six horses treated alternately with either clopidogrel or aspirin for a period of five days.
- The dosage for clopidogrel was 2mg/kg orally every 24 hours, while the dosage for aspirin was 5mg/kg orally every day.
Methodology and Data Collection
- Platelets were collected from the horses at distinct periods—24, 72, 96, 120, and 168 hours after the beginning of therapy.
- These platelets were then stimulated using 0.1 μg/mL thrombin and analyzed via flow cytometry with the help of anti-CD41/61 and anti-equine CD62P antibodies.
- The number of platelet-PMN aggregates was also calculated. PMN refers to polymorphonuclear leukocytes, a kind of white blood cell.
Results
- No significant differences were found in the baseline numbers of platelets showing CD62P positivity between the two groups.
- While a tendency for decreased expression was observed in both groups post treatment, no significant changes in the count of CD62P positive platelets were seen with either drug.
- There were also no changes in the formation of platelet-PMN aggregates during or after treatment with either aspirin or clopidogrel.
Limitations
- The study acknowledges that due to the small number of horses used, it may have been underpowered to detect a definitive decrease in expression.
- The authors also suggested that differences between the treatments might have been more noticeable if the study had been carried out on horses with systemic inflammation.
Conclusions
- This study concluded that treatment with either aspirin or clopidogrel does not affect P-selectin expression on equine platelets and does not significantly impact the formation of platelet-neutrophil aggregates.
Cite This Article
APA
Brainard BM, Epstein KL, LoBato DN, Kwon S, Darien BJ, Hurley DJ, Moore JN.
(2012).
Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates.
Vet Immunol Immunopathol, 149(1-2), 119-125.
https://doi.org/10.1016/j.vetimm.2012.05.022 Publication
Researcher Affiliations
- College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Dr., Athens, GA 30602, USA. brainard@uga.edu
MeSH Terms
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
- Anti-Inflammatory Agents, Non-Steroidal / pharmacokinetics
- Aspirin / administration & dosage
- Aspirin / pharmacokinetics
- Blood Platelets / cytology
- Blood Platelets / drug effects
- Blood Platelets / immunology
- Clopidogrel
- Cross-Over Studies
- Female
- Flow Cytometry / veterinary
- Horses / blood
- Horses / immunology
- Male
- Neutrophils / cytology
- Neutrophils / drug effects
- Neutrophils / immunology
- P-Selectin / biosynthesis
- P-Selectin / blood
- P-Selectin / immunology
- Platelet Aggregation / drug effects
- Platelet Aggregation / immunology
- Random Allocation
- Statistics, Nonparametric
- Ticlopidine / administration & dosage
- Ticlopidine / analogs & derivatives
- Ticlopidine / pharmacokinetics
Citations
This article has been cited 1 times.- Theuerkauf K, Obach-Schröck C, Staszyk C, Moritz A, Roscher KA. Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome. J Vet Diagn Invest 2022 May;34(3):448-457.
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