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Home / Equine Research Bank / Reprod Domest Anim / Tribulus terrestris can suppress the adverse effect of tolue...
Reproduction in domestic animals = Zuchthygiene2022; 57(11); 1307-1318; doi: 10.1111/rda.14204

Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells.

Abstract: Influence of oil-related product toluene and herbal remedy puncturevine Tribulus terrestris L. (TT) on female reproduction is known. Yet, mechanisms of their action on ovaries in different species and potential protective effect of TT against adverse toluene action remain to be established. We studied the effect of toluene, TT, and their combination on ovarian granulosa cells from two mammalian species (cows and horses). Viability, markers of proliferation (PCNA) and apoptosis (bax), steroid hormones, IGF-I, oxytocin, and prostaglandin F (PGF) release were analyzed by trypan blue exclusion test, quantitative immunocytochemistry, and EIA/ELISA. Toluene suppressed all analyzed parameters. In both species, TT stimulated proliferation and reduced progesterone, oxytocin, and PGF. In horses, TT inhibited testosterone and IGF-I. In both species, TT supported toluene effect on viability, steroids, IGF-I, and PGF, and inverted its action on apoptosis. In cows, TT promoted toluene effect on proliferation. In horses, TT supported toluene effect on oxytocin but suppressed its influence on proliferation. In both species, toluene induced inhibitory action of TT on viability, steroids, IGF-I, and PGF, and prevented its stimulatory action on proliferation. In cows, toluene supported inhibitory action of TT on oxytocin and prevented its stimulatory action on apoptosis. In horses, toluene induced stimulatory effect of TT on apoptosis. Our results indicate potential toxic toluene effect on farm animal ovaries, applicability of TT as a biostimulator of farm animal reproduction and as a protector against the adverse influence of toluene on female reproduction.
© 2022 Wiley-VCH GmbH.
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Publication Date: 2022-07-13 PubMed ID: 35789053DOI: 10.1111/rda.14204Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research demonstrates that the plant Tribulus terrestris can potentially help mitigate the negative effects of the chemical toluene on granulosa cells in the ovaries of cows and horses.

Introduction and Objectives

  • This study investigated the impacts of the chemical toluene and the herb Tribulus terrestris on the reproduction function of two mammalian species – cows and horses. Specifically, it focused on their effect on the ovarian granulosa cells which play significant roles in female reproduction.
  • The researchers aimed to understand the mechanisms of action of toluene and Tribulus terrestris on ovaries across different species, and whether the latter could counteract any adverse effects of toluene.

Methods

  • The research team examined the effects of toluene and Tribulus terrestris, individually and in combination, on a variety of parameters associated with the function of granulosa cells. These parameters included cell viability, markers for cell proliferation (PCNA) and induced cell death (bax), along with levels of several hormones and other significant compounds (steroid hormones, IGF-I, oxytocin, and prostaglandin F).
  • These effects were analyzed using the trypan blue exclusion test for cell viability, quantitative immunocytochemistry for PCNA and bax markers, and Enzyme-linked immunoassay (EIA/ELISA) methods for hormone level assessments.

Results

  • The study found that toluene suppressed all analyzed parameters, indicating its potentially harmful effect on the reproductive function of cows and horses.
  • It was observed that Tribulus terrestris, on the other hand, stimulated cell proliferation and decreased levels of progesterone, oxytocin, and prostaglandin F. In horses, it also inhibited testosterone and IGF-I.
  • When toluene and Tribulus terrestris were administered together, Tribulus terrestris was found to support the effects of toluene on cell viability, steroidal hormones, IGF-I, and prostaglandin F, but reversed its impacts on cell death. This indicated that Tribulus terrestris could counteract adverse effects of toluene on granulosa cell viability and hormonal balance.

Conclusion

  • The results of this study highlight the potential toxicity of toluene on the ovaries of farm animals. It also demonstrates the potential of Tribulus terrestris as a biostimulator for farm animal reproduction, and as a protective agent against the detrimental effects of toluene on female reproduction.

Cite This Article

APA
Fabová Z, Tarko A, Harrath AH, Alwasel S, Kotwica J, Sirotkin AV. (2022). Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells. Reprod Domest Anim, 57(11), 1307-1318. https://doi.org/10.1111/rda.14204

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 57
Issue: 11
Pages: 1307-1318

Researcher Affiliations

Fabová, Zuzana
  • Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University, Nitra, Slovak Republic.
Tarko, Adam
  • Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University, Nitra, Slovak Republic.
Harrath, Abdel Halim
  • Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Alwasel, Saleh
  • Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Kotwica, Jan
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Sirotkin, Alexander V
  • Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University, Nitra, Slovak Republic.

MeSH Terms

  • Cattle
  • Horses
  • Animals
  • Female
  • Tribulus
  • Insulin-Like Growth Factor I / pharmacology
  • Toluene / toxicity
  • Oxytocin / pharmacology
  • Cell Proliferation
  • Granulosa Cells
  • Progesterone / pharmacology
  • Apoptosis
  • Prostaglandins F
  • Cells, Cultured
  • Mammals

Grant Funding

  • Vedecku00e1 Grantovu00e1 Agentu00fara Mu0160VVau0160 SR a SAV

References

This article includes 58 references
  1. Abadjieva D, Kistanova E. Tribulus terrestris alters the expression of growth differentiation factor 9 and bone morphogenetic protein 15 in rabbit ovaries of mothers and F1 female offspring. PLoS One 11 e0150400.
  2. Adaay M H, Mosa A R. Evaluation of the effect of aqueous extract of Tribulus terrestris on some reproductive parameters in female mice. Journal of Materials and Environmental Science 3 1153-1162.
  3. Arentz S, Abbott J A, Smith C A, Bensoussan A. Herbal medicine for the management of polycystic ovary syndrome (PCOS) and associated oligo/amenorrhoea and hyperandrogenism; a review of the laboratory evidence for effects with corroborative clinical findings. BMC Complementary and Alternative Medicine 14 511.
  4. Ashraf S S, Rao M V, Kaneez F S, Qadri S, Al-Marzouqi A H, Chandranath I S, Adem A. Nigella sativa extract as a potent antioxidant for petrochemical-induced oxidative stress. Journal of Chromatographic Science 49 321-326.
  5. Aslam M. Protective effect of Tribulus terrestris on nifedipine induced damage to microstructure of testes of rats. Journal of Islamic International Medical College 13 151-126.
  6. Bertozzo T V, Moreira L R, de Angelo J M, MCL M. Variation of weight and dosage of testosterone, progesterone and estradiol of ovariectomized and not ovariectomized female Swiss mice supplements with extract of Tribulus terrestris L. Salusvita 38 665-683.
  7. Bolden A L, Kwiatkowski C F, Colborn T. New look at BTEX: Are ambient levels a problem?. Environmental Science & Technology 49 5261-5276.
  8. Chen H, Wang X, Xu L. Zhonghua yu fang yi xue za zhi. Chinese Journal of Preventive Medicine 35 83-86.
  9. Chhatre S, Nesari T, Somani G, Kanchan D, Sathaye S. Phytopharmacological overview of Tribulus terrestris. Pharmacognosy Reviews 8 45-51.
  10. Das N, Kumar T R. Molecular regulation of follicle-stimulating hormone synthesis, secretion and action. Journal of Molecular Endocrinology 60 R131-R155.
  11. Dehghan A, Esfandiari A, Bigdeli S M. Alternative treatment of ovarian cysts with Tribulus terrestris extract: A rat model. Reproduction in Domestic Animals 47 e12-e15.
  12. Donald J M, Hooper K, Hopenhayn-Rich C. Reproductive and developmental toxicity of toluene: A review. Environmental Health Perspectives 94 237-244.
  13. Eagappan K, Sasikumar S, Shah D B, Aruna V N. Impact of cooked Tribulus terrestris fruit extract on lead induced hepato and renaltoxicity. American Journal of Ethnomedicine 2 1-13.
  14. Gama C R, Lasmar R, Gama G F, Abreu C S, Nunes C P, Geller M, Oliveira L, Santos A. Clinical assessment of Tribulus terrestris extract in the treatment of female sexual dysfunction. Clinical Medicine Insights: Women's Health 7 45-50.
  15. Gaumer S, Guénal I, Brun S, Théodore L, Mignotte B. Bcl-2 and Bax mammalian regulators of apoptosis are functional in Drosophila. Cell Death and Differentiation 7 804-814.
  16. Homanics G E, Silvia W J. Effects of progesterone and estradiol-17 beta on uterine secretion of prostaglandin F2 alpha in response to oxytocin in ovariectomized ewes. Biology of Reproduction 38 804-811.
  17. Jameel M, Ansari J A, Abuzer A, Javed A, Mohammed A, Ennus T T. Pharmacological scientific evidence for the promise of Tribulus terrestris. International Research Journal of Pharmacy 3 403-406.
  18. Johnson I T, Gee J M, Price K, Curl C, Fenwick G R. Influence of saponins on gut permeability and active nutrient transport in vitro. The Journal of Nutrition 116 2270-2277.
  19. Kareem L A, Banna H B, Naoom K M. Effects of toluene and formaldehyde on oogenesis in adult female mice. Diyala Journal of Medicine 6 33-44.
  20. Keshtmand Z, Oryan S, Ghanbari A, Khazaei M. Protective effect of Tribulus terrestris hydroalcoholic extract against cisplatin-induced cytotoxicity on sperm parameters in male mice. International Journal of Morphology 32 551-557.
  21. Kitazumi I, Tsukahara M. Regulation of DNA fragmentation: the role of caspases and phosphorylation. The FEBS Journal 278 427-441.
  22. Kotwica J, Skarzynski D. Influence of oxytocin removal from the corpus luteum on secretory function and duration of the oestrous cycle in cattle. Journal of Reproduction and Fertility 97 411-417.
  23. Kotwica J, Skarzynski D J, Jaroszewski J J, Bogacki M. Noradrenaline affects secretory function of corpus luteum independently on prostaglandins in conscious cattle. Prostaglandins 48 1-10.
  24. Lakshmi G D, Kumar P R, Bharavi K, Annapurna P, Rajendar B, Patel P T, Kumar C S, Rao G S. Protective effect of Tribulus terrestris linn on liver and kidney in cadmium intoxicated rats. Indian Journal of Experimental Biology 50 141-146.
  25. Ng T P, Foo S C, Yoong T. Risk of spontaneous abortion in workers exposed to toluene. British Journal of Industrial Medicine 49 804-808.
  26. Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N. Crosstalk between apoptosis, necrosis and autophagy. Biochimica et Biophysica Acta 1833 3448-3459.
  27. Osborn M, Brandfass S. Immunocytochemistry of frozen and of paraffin tissue sections. Cell Biology 1 563-569.
  28. Peña-Blanco A, García-Sáez A J. Bax, Bak and beyond - mitochondrial performance in apoptosis. The FEBS Journal 285 416-431.
  29. Raoofi A, Khazaei M, Ghanbari A. Protective effect of hydroalcoholic extract of tribulus terrestris on Cisplatin induced renal tissue damage in male mice. International Journal of Preventive Medicine 6 11.
  30. . Report on Carcinogens. 2016 National Toxicology Program.
  31. Reutman S R, LeMasters G K, Knecht E A, Shukla R, Lockey J E, Burroughs G E, Kesner J S. Evidence of reproductive endocrine effects in women with occupational fuel and solvent exposures. Environmental Health Perspectives 110 805-811.
  32. Roberts L G, Bevans A C, Schreiner C A. Developmental and reproductive toxicity evaluation of toluene vapor in the rat. I. Reproductive toxicity. Reproductive Toxicology 17 649-658.
  33. Roberts L G, Nicolich M J, Schreiner C A. Developmental and reproductive toxicity evaluation of toluene vapor in the rat. II. Developmental toxicity. Reproductive Toxicology 23 521-531.
  34. Robker R L, Richards J S. Hormone-induced proliferation and differentiation of granulosa cells: A coordinated balance of the cell cycle regulators cyclin D2 and p27Kip1. Molecular Endocrinology 12 924-940.
  35. Saiyed A, Jahan N, Makbul S, Ansari M, Bano H, Habib S H. Effect of combination of Withania somnifera Dunal and Tribulus terrestris Linn on letrozole induced polycystic ovarian syndrome in rats. Integrative Medicine Research 5 293-300.
  36. Sallmén M, Neto M, Mayan O N. Reduced fertility among shoe manufacturing workers. Occupational and Environmental Medicine 65 518-524.
  37. Sandeep P M, Bovee T F, Sreejith K. Anti-androgenic activity of Nardostachys jatamansi DC and Tribulus terrestris L. and their beneficial effects on polycystic ovary syndrome-induced rat models. Metabolic Syndrome and Related Disorders 13 248-254.
  38. Schieber M, Chandel N S. ROS function in redox signaling and oxidative stress. Current Biology 24 R453-R462.
  39. Shiomi Y, Nishitani H. Control of genome integrity by RFC complexes; conductors of PCNA loading onto and unloading from chromatin during DNA replication. Genes 8 52.
  40. Sirotkin AV. Regulators of ovarian functions. Nova Biomedical 193 ISBN 978-1-61324-468-5.
  41. Sirotkin A V, Alexa R, Alwasel S, Harrath A H. The phytoestrogen, diosgenin, directly stimulates ovarian cell functions in two farm animal species. Domestic Animal Endocrinology 69 35-41.
  42. Sirotkin A V, Alexa R, Harrath A H. Puncturevine (Tribulus terrestris L.) affects the proliferation, apoptosis, and ghrelin response of ovarian cells. Reproductive Biology 20 33-36.
  43. Sirotkin A V, Stochmalova A, Scsukova S. Plant isoflavones can affect accumulation and impact of silver and titania nanoparticles on ovarian cells. Endocrine Regulations 55 52-60.
  44. Sirotkin A V, Hrabovszká S, Štochmaľová A, Grossmann R, Alwasel S, Harrath A H. Effect of quercetin on ovarian cells of pigs and cattle. Animal Reproduction Science 205 44-51.
  45. Sirotkin A V, Kadasi A, Baláži A, Kotwica J, Alrezaki A, Harrath A H. Mechanisms of the direct effects of oil-related contaminants on ovarian cells. Environmental Science and Pollution Research International 27 5314-5322.
  46. Sirotkin A V, Makarevich A V, Kubovicova E, Medvedova M, Kolesarova A, Harrath A H. Relationship between body conditions and environmental contaminants in bovine ovarian cells. Theriogenology 147 77-84.
  47. Sirotkin A V, Štochmaľová A, Alexa R, Kádasi A, Bauer M, Grossmann R, Alrezaki A, Alwasel S, Harrath A H. Quercetin directly inhibits basal ovarian cell functions and their response to the stimulatory action of FSH. European Journal of Pharmacology 860 172560.
  48. Sirotkin A V, Záhoranska Z, Tarko A, Fabova Z, Alwasel S, Halim Harrath A. Plant polyphenols can directly affect ovarian cell functions and modify toluene effects. Journal of Animal Physiology and Animal Nutrition 105 80-89.
  49. Sirotkin A V, Záhoranska Z, Tarko A, Popovska-Percinic F, Alwasel S, Harrath A H. Plant isoflavones can prevent adverse effects of benzene on porcine ovarian activity: An in vitro study. Environmental Science and Pollution Research International 27 29589-29598.
  50. Soffritti M, Belpoggi F, Degli Esposti D, Lambertini L, Tibaldi E, Rigano A. First experimental demonstration of the multipotential carcinogenic effects of aspartame administered in the feed to Sprague-Dawley rats. Environmental Health Perspectives 114 379-385.
  51. Strober W. Trypan blue exclusion test of cell viability. Current Protocols in Immunology 111 A3.B.1-A3.B.3.
  52. Tap O, Solmaz S, Polat S, Mete U O, Ozbilgïn M K, Kaya M. The effect of toluene on the rat ovary: An ultrastructural study. Journal of Submicroscopic Cytology and Pathology 28 553-558.
  53. Tarko A, Fabová Z, Kotwica J, Valocký I, Alrezaki A, Alwasel S, Harrath A H, Sirotkin A V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. General and Comparative Endocrinology 295 113491.
  54. Tarko A, Štochmalova A, Hrabovszka S, Vachanova A, Harrath A H, Alwasel S, Grossman R, Sirotkin A V. Can xylene and quercetin directly affect basic ovarian cell functions?. Research in Veterinary Science 119 308-312.
  55. Valko M, Izakovic M, Mazur M, Rhodes C J, Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Molecular and Cellular Biochemistry 266 37-56.
  56. Voronina E, Lovasco L A, Gyuris A, Baumgartner R A, Parlow A F, Freiman R N. Ovarian granulosa cell survival and proliferation requires the gonad-selective TFIID subunit TAF4b. Developmental Biology 303 715-726.
  57. Waldner C L. The association between exposure to the oil and gas industry and beef calf mortality in Western Canada. Archives of Environmental & Occupational Health 63 220-240.
    doi: 10.3200/aeoh.63.4.220-240google scholar: lookup
  58. WHO. Petroleum products in drinking water. WHO guideliness for drinking water quality .

Citations

This article has been cited 2 times.
  1. Han R, Li J, Hony J, Xiao Z, Wang J, Yao M, Liang S, Lu L. CAXII inhibitors: Potential sensitizers for immune checkpoint inhibitors in HCC treatment. Front Immunol 2023;14:1052657.
    doi: 10.3389/fimmu.2023.1052657pubmed: 37006233google scholar: lookup
  2. Malik MY, Alex A, Sivalingam AM, Neha B, Vimal S. Evaluation of the Phytochemical Screening of Methanolic Seed Extracts of Tribulus terrestris: An In Vitro Application of Anti-cancer, Anti-oxidant, and Anti-microbial Activities. Cureus 2024 Aug;16(8):e66674.
    doi: 10.7759/cureus.66674pubmed: 39262564google scholar: lookup
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