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Home / Equine Research Bank / J Vet Intern Med / Tumor necrosis factor and interleukin-6 activity and endotox...
Journal of veterinary internal medicine1999; 13(5); 457-464; doi: 10.1892/0891-6640(1999)013<0457:tnfaia>2.3.co;2

Tumor necrosis factor and interleukin-6 activity and endotoxin concentration in peritoneal fluid and blood of horses with acute abdominal disease.

Abstract: The purpose of this study was to evaluate the diagnostic and prognostic significance of tumor necrosis factor-alpha (TNF) and interleukin-6 (IL-6) activities and endotoxin concentration in blood and peritoneal fluid of 155 adult horses with acute abdominal disease (colic). Samples also were obtained from 20 healthy adult horses. Blood and peritoneal fluid supernatant TNF and IL-6 activities and endotoxin concentration were significantly greater in horses with colic, compared with healthy horses. In horses with colic, the peritoneal fluid endotoxin concentration and TNF and IL-6 activities were significantly greater than those in blood. Within the colic group, peritoneal fluid IL-6 activity was the analyte that was most frequently increased. Blood and peritoneal fluid supernatant TNF and IL-6 activities were significantly greater when endotoxin was detected in the same sample. Blood and peritoneal fluid IL-6 activity was significantly greater in horses with inflammatory or strangulating lesions, compared with horses having nonstrangulating or noninflammatory lesions. Compared with all other data categories, diagnostic accuracy for nonsurvival was greatest (80%) when blood IL-6 activity exceeded 60 units/mL. The results of this study indicate that endotoxin was present in the peritoneal cavity of at least one third of horses with any acute disease of the abdomen. In horses presented for colic, blood or peritoneal fluid IL-6 activity was more useful than either TNF activity or endotoxin concentration for distinguishing lesion type. Although diagnostic accuracy for the prediction of nonsurvival was good for all of the analytes, negative values were more useful in the prediction of a favorable outcome than were abnormally increased values in the prediction of mortality.
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Publication Date: 1999-09-28 PubMed ID: 10499730DOI: 10.1892/0891-6640(1999)013<0457:tnfaia>2.3.co;2Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to understand the role of certain bio-markers in equine abdominal disease, finding notable increases in Tumor Necrosis Factor, Interleukin-6, and endotoxin levels in horses with colic, compared to healthy ones. Of these, Interleukin-6 was particularly useful in diagnosing lesion type and predicting survival outcomes.

Objective of the Research

  • The primary objective of this study was to assess the diagnostic and prognostic value of Tumor Necrosis Factor-alpha (TNF), Interleukin-6 (IL-6) activities and endotoxin concentration in the blood and peritoneal fluid of adult horses experiencing acute abdominal disease (colic).

Methodology

  • The researchers collected samples from 155 adult horses diagnosed with colic and 20 healthy adult horses for comparison.
  • They specifically measured the activities of TNF and IL-6, as well as the concentration of endotoxin, in both the blood and the peritoneal fluid of the subjects.

Key Findings

  • The research found that activities of TNF and IL-6 and the concentration of endotoxins were significantly higher in horses with colic compared to healthy ones.
  • Within the colic group, horses had higher IL-6 levels and endotoxin concentration in their peritoneal fluid compared to their blood.
  • Among horses with colic, IL-6 prevalence in their peritoneal fluid was the most frequently increased factor.
  • Increased TNF and IL-6 activities were significantly correlated with the detection of endotoxins in the same sample.
  • The levels of IL-6 in both blood and peritoneal fluid were higher in horses with inflammatory or strangulating lesions, compared to those with non-strangulating or non-inflammatory lesions.
  • The researchers found that the likelihood of non-survival was greatest—80%—when blood IL-6 activity exceeded 60 units/mL.

Conclusion

  • Based on the findings, the study concluded that endotoxins were present in the peritoneal cavity of at least one third of horses with any acute disease of the abdomen.
  • In diagnosing colic, the study indicates that IL-6 activity is more useful than TNF activity or endotoxin concentration in distinguishing the type of lesion.
  • While all the analyzed factors had a good degree of accuracy in predicting survival, the research found that negative values were more useful in predicting favorable outcomes than abnormally increased values were in predicting mortality.

Cite This Article

APA
Barton MH, Collatos C. (1999). Tumor necrosis factor and interleukin-6 activity and endotoxin concentration in peritoneal fluid and blood of horses with acute abdominal disease. J Vet Intern Med, 13(5), 457-464. https://doi.org/10.1892/0891-6640(1999)013<0457:tnfaia>2.3.co;2

Publication

Journal of veterinary internal medicine
ISSN: 0891-6640
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 13
Issue: 5
Pages: 457-464

Researcher Affiliations

Barton, M H
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA. mbarton@calc.vet.uga.edu
Collatos, C

    MeSH Terms

    • Abdomen, Acute / diagnosis
    • Abdomen, Acute / mortality
    • Abdomen, Acute / veterinary
    • Animals
    • Ascitic Fluid / metabolism
    • Ascitic Fluid / veterinary
    • Case-Control Studies
    • Endotoxins / blood
    • Endotoxins / metabolism
    • Horse Diseases / diagnosis
    • Horse Diseases / metabolism
    • Horse Diseases / mortality
    • Horses
    • Interleukin-6 / blood
    • Interleukin-6 / metabolism
    • Predictive Value of Tests
    • Prognosis
    • Survival Analysis
    • Tumor Necrosis Factor-alpha / metabolism

    Citations

    This article has been cited 13 times.
    1. Mercer MA, Davis JL, McKenzie HC. The Clinical Pharmacology and Therapeutic Evaluation of Non-Steroidal Anti-Inflammatory Drugs in Adult Horses.. Animals (Basel) 2023 May 10;13(10).
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    2. van Spijk JN, Beckmann K, Wehrli Eser M, Stirn M, Steuer AE, Saleh L, Schoster A. Preliminary Investigation of Side Effects of Polymyxin B Administration in Hospitalized Horses.. Antibiotics (Basel) 2023 May 5;12(5).
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    4. Edes AN, Zimmerman D, Jourdan B, Brown JL, Edwards KL. Value Ranges and Clinical Comparisons of Serum DHEA-S, IL-6, and TNF-α in Western Lowland Gorillas.. Animals (Basel) 2022 Oct 8;12(19).
      doi: 10.3390/ani12192705pubmed: 36230446google scholar: lookup
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      doi: 10.1111/jvim.16470pubmed: 35801274google scholar: lookup
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      doi: 10.1111/vsu.13839pubmed: 35674231google scholar: lookup
    7. Barton AK, Richter IG, Ahrens T, Merle R, Alalwani A, Lilge S, Purschke K, Barnewitz D, Gehlen H. MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic.. Mediators Inflamm 2021;2021:9501478.
      doi: 10.1155/2021/9501478pubmed: 33488296google scholar: lookup
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    11. Lewis DH, Chan DL, Pinheiro D, Armitage-Chan E, Garden OA. The immunopathology of sepsis: pathogen recognition, systemic inflammation, the compensatory anti-inflammatory response, and regulatory T cells.. J Vet Intern Med 2012 May-Jun;26(3):457-82.
      doi: 10.1111/j.1939-1676.2012.00905.xpubmed: 22428780google scholar: lookup
    12. Lillich JD, Ray-Miller W, Silver KS, Davis EG, Schultz BD. Intra-abdominal hyaluronan concentration in peritoneal fluid of horses with sudden signs of severe abdominal pain.. Am J Vet Res 2011 Dec;72(12):1666-73.
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