Ultrafiltration of equine digital lamellar tissue.
Abstract: There are no experimentally validated pharmacological means of preventing laminitis; however, locally acting pharmaceutical agents with the potential to prevent laminitis have been identified. Demonstrating therapeutic drug concentrations in lamellar tissue is essential for evaluating the efficacy of these agents. The aim of this study was to develop an experimental technique for repeatedly sampling lamellar interstitial fluid. A technique for placing ultrafiltration probes was developed in vitro using 15 cadaver limbs. Subsequently, lamellar ultrafiltration probes were placed in one forelimb in six living horses. Interstitial fluid was collected continuously from the probes as ultrafiltrate for 4 (n = 4) or 14 days (n = 2). The rate of ultrafiltrate collection was calculated every 12 h. Biochemical analyses were performed on ultrafiltrate collected on night 1 (12-24 h post-implantation) and night 4 (84-96 h post-implantation). Sections surrounding the probe and control tissue from the contralateral limb were harvested, stained with H&E and Masson's trichrome and scored based on the tissue response to the probe. Ultrafiltration probes were placed in the lamellar tissue in all six horses. Ultrafiltrate was collected from these probes at 55 (30-63) μL/h (median [interquartile range]). Fluid production decreased significantly with time from night 3 onwards (P 0.05). The technique was well tolerated. This study demonstrates that ultrafiltration can be used to sample equine digital lamellar interstitial fluid, and has potential for measuring lamellar drug levels.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication Date: 2014-05-09 PubMed ID: 25439438DOI: 10.1016/j.tvjl.2014.05.007Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explores a new method for sampling fluid from the lamellar tissue of horses, which could advance the study of laminitis prevention protocols in equine sciences.
Research Objectives
- The primary objective of the study was to devise a new experimental technique to sample the interstitial fluid from the lamellar tissue in horses. This fluid is crucial in understanding and evaluating the effectiveness of local pharmaceutical agents in preventing laminitis, a serious equine condition.
Methodology
- The researchers developed an ultrafiltration probe placement technique using 15 cadaver limbs before implementing the procedure live horses.
- Ultrafiltration probes were inserted into one forelimb in six live horses.
- Interstitial fluid was then collected non-stop from these probes for either four or fourteen days.
- The rate of ultrafiltrate collection was calculated every 12 hours.
- Biochemical analyses were carried out on the collected ultrafiltrate at different intervals: after 12-24 hours and 84-96 hours post-implantation.
- Usage bioinformatics: Tissue surrounding the probe and control tissue from the other limb were stained and scored based on the tissue’s reaction to the probe.
Results
- Successful insertion of ultrafiltration probes was achieved in all six horses. The median rate of fluid collection was around 55 µL/h
- The study observed a significant decrease in fluid production from the third night onwards.
- No substantial change was noted in the constituents of the ultrafiltrate between the first and fourth nights.
- The study concludes that the technique was well tolerated by the horses.
Conclusion
- The research conclusively established that ultrafiltration could be a potential method to sample equine digital lamellar interstitial fluid.
- Advancements such as this could further the study of lamellar drug levels, contributing significantly to prevention methods for equine laminitis.
Cite This Article
APA
Underwood C, Collins SN, van Eps AW, Allavena RE, Medina-Torres CE, Pollitt CC.
(2014).
Ultrafiltration of equine digital lamellar tissue.
Vet J, 202(2), 314-322.
https://doi.org/10.1016/j.tvjl.2014.05.007 Publication
Researcher Affiliations
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia. Electronic address: c.underwood1@uq.edu.au.
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia.
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia.
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia.
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia.
- Australian Equine Laminitis Research Unit, School of Veterinary Science, University of Queensland, Gatton Campus, Gatton, Qld 4343, Australia.
MeSH Terms
- Animals
- Chromatography, High Pressure Liquid / veterinary
- Extracellular Fluid / chemistry
- Hoof and Claw / chemistry
- Horses / metabolism
- Lameness, Animal / etiology
- Lameness, Animal / physiopathology
- Male
- Pain Measurement / veterinary
- Pharmaceutical Preparations / analysis
- Time Factors
- Ultrafiltration / veterinary
Citations
This article has been cited 4 times.- Koch DW, Berglund AK, Messenger KM, Gilbertie JM, Ellis IM, Schnabel LV. Interleukin-1β in tendon injury enhances reparative gene and protein expression in mesenchymal stem cells. Front Vet Sci 2022;9:963759.
- Ellis I, Schnabel LV, Berglund AK. Defining the Profile: Characterizing Cytokines in Tendon Injury to Improve Clinical Therapy. J Immunol Regen Med 2022 May;16.
- Enomoto H, Yeatts J, Carbajal L, Krishnan BR, Madan JP, Laumas S, Blikslager AT, Messenger KM. In vivo assessment of a delayed release formulation of larazotide acetate indicated for celiac disease using a porcine model. PLoS One 2021;16(4):e0249179.
- Munn R, Whittem T. Moxidectin is a candidate for use as an in vivo internal standard in pharmacokinetic studies, as demonstrated with use in simultaneous tissue cage and ultrafiltration fluid collection. Front Vet Sci 2024;11:1332974.
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