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Reproduction in domestic animals = Zuchthygiene2020; 55(4); 496-502; doi: 10.1111/rda.13643

Upregulation of CRISP-3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage.

Abstract: For unknown reasons, stallion fertility and sperm longevity during cooled storage of semen vary markedly between individuals. Spermatozoa from individual stallions react differently to the presence, or the removal, of seminal plasma (SP). The aim was to evaluate differences in protein content in stallion seminal plasma with either a positive or a negative effect on sperm chromatin integrity during storage. Stallion semen samples from different ejaculate fractions were stored at 5°C for 24 hr. Sperm survival was assessed after storage using a sperm chromatin structure assay. Protein expression in SP with either positive or negative effects on sperm survival during storage was studied using two-dimensional differential gel electrophoresis and liquid chromatography-mass spectrometry. Lower sperm chromatin integrity was associated with upregulation of the proteins kallikrein, CRISP-3 and HSP-1, while higher chromatin integrity was associated with upregulation of TIMP-2. In the sperm-rich fractions, kallikrein and CRISP-3 differed significantly between SP samples with differing effects on sperm chromatin integrity. In the sperm-poor fractions, TIMP-2 and HSP-1 differed significantly between the two SP groups. Differences in the seminal plasma proteome are associated with sperm longevity during cooled storage.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-02-03 PubMed ID: 31965650DOI: 10.1111/rda.13643Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the relationship between the protein content in stallion seminal plasma (SP) and sperm longevity and fertility, particularly during cold storage. It revealed an association between higher levels of certain proteins (kallikrein, CRISP-3, and HSP-1) and lower sperm chromatin integrity, while an upregulation of TIMP-2 was associated with better integrity. Such differences within the seminal plasma proteome seem to play a role in sperm longevity during cooled storage.

Objective of the Study

  • The primary aim of this research was to identify variations in protein content of stallion seminal plasma (SP) which could have an influence, either positive or negative, on sperm chromatin integrity during storage.
  • The researchers were keen on determining why the fertility rates and longevity of stallion sperm differs significantly between individuals, particularly during the cooled storage of semen.

Methods Used

  • Semen samples from different ejaculate fractions of stallions were stored at 5°C for 24 hours.
  • Following the storage period, a sperm chromatin structure assay was used to assess sperm viability.
  • To evaluate the differences in protein expression the researchers used two-dimensional differential gel electrophoresis along with liquid chromatography-mass spectrometry.

Findings of the Study

  • The study established that decreased sperm chromatin integrity was associated with elevated levels of the proteins kallikrein, CRISP-3, and HSP-1.
  • On the other hand, better sperm chromatin integrity was associated with increased levels of TIMP-2.
  • In considering sperm-rich fractions, noticeable differences were observed between SP samples with varying levels of kallikrein and CRISP-3 in relation to sperm chromatin integrity.
  • For sperm-poor fractions, significant differences were observed between the two SP groups in terms of the levels of TIMP-2 and HSP-1.

Conclusion

  • The study concludes that there are notable differences within the seminal plasma proteome (protein composition) that appear to be associated with stallion sperm longevity during cooled storage.
  • These differences can thus have a direct influence on the fertility and the viability of the stored sperm, hence elucidating why individual stallions exhibit varying levels of fertility and sperm longevity.

Cite This Article

APA
Kareskoski AM, Palviainen M, Johannisson A, Katila T. (2020). Upregulation of CRISP-3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage. Reprod Domest Anim, 55(4), 496-502. https://doi.org/10.1111/rda.13643

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 4
Pages: 496-502

Researcher Affiliations

Kareskoski, Anna Maria
  • Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Saarentaus, Finland.
Palviainen, Mari
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, Central Laboratory, University of Helsinki, Helsinki, Finland.
Johannisson, Anders
  • Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Katila, Terttu
  • Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Saarentaus, Finland.

MeSH Terms

  • Animals
  • Chromatin / physiology
  • Cold Temperature / adverse effects
  • Horses / physiology
  • Kallikreins / analysis
  • Male
  • Semen / chemistry
  • Semen Preservation / adverse effects
  • Semen Preservation / methods
  • Semen Preservation / veterinary
  • Seminal Plasma Proteins / analysis
  • Spermatozoa / physiology

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Citations

This article has been cited 1 times.
  1. Rodriguez-Martinez H, Martinez EA, Calvete JJ, Peña Vega FJ, Roca J. Seminal Plasma: Relevant for Fertility?. Int J Mol Sci 2021 Apr 22;22(9).
    doi: 10.3390/ijms22094368pubmed: 33922047google scholar: lookup
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