Upregulation of mRNA of interleukin-1 and -6 in subchondral cystic lesions of four horses.
Abstract: This study investigated the potential association of interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) in subchondral cystic lesions (SCL) in horses. With the technique of in situ hybridisation in paraffin sections of fibrous tissue of SCL and quantitative real-time PCR in fresh frozen fibrous tissue and undecalcified bone sections of SCL embedded in acrylic resin, upregulation of mRNA of both cytokines could be demonstrated. mRNA of IL-1beta was upregulated at the periphery of the cystic lesion adjacent to normal bone, whereas IL-6 mRNA was upregulated within the fibrous tissue found within the centre of the SCL. It was concluded that both cytokines are associated in pathological bone resorption observed in SCL and, in combination with increased production of prostaglandin E2, may be responsible for the slow healing, maintenance or further expansion of the cystic lesions.
Publication Date: 2001-03-27 PubMed ID: 11266063DOI: 10.1111/j.2042-3306.2001.tb00592.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article discusses the potential link between interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) in subchondral cystic lesions (SCL) in horses. The investigation revealed an upregulation of mRNA of both cytokines within these lesions.
Methodology
- The researchers used a technique called in situ hybridisation to examine paraffin sections of fibrous tissues within the lesions. This method allows for the investigating of specific DNA or RNA sequences within a tissue.
- Quantitative real-time PCR (polymerase chain reaction) was used for fresh frozen fibrous tissue and undecalcified bone sections of the lesions that were embedded in acrylic resin. PCR is a common lab technique used to amplify and measure the amount of a specific DNA sequence.
Findings
- Both methods demonstrated an upregulation of mRNA of IL-1beta and IL-6. Upregulation refers to the process by which a cell increases the quantity of a cellular component, in this case, the mRNA of these cytokines within the lesions.
- It was observed that mRNA of IL-1beta was upregulated at the periphery of the cystic lesion adjacent to normal bone.
- On the other hand, IL-6 mRNA was upregulated within the fibrous tissue found within the center of the SCL.
Conclusion
- The researchers concluded that both cytokines are associated with the pathological bone resorption observed in SCL. Pathological bone resorption refers to an abnormal condition where the body’s natural bone reabsorption process (which allows for bone remodeling and healing) is disrupted.
- The findings suggest that IL-1beta and IL-6, along with an increased production of prostaglandin E2, could be responsible for the slow healing, maintenance, or further expansion of the cystic lesions.
Cite This Article
APA
von Rechenberg B, Leutenegger C, Zlinsky K, McIlwraith CW, Akens MK, Auer JA.
(2001).
Upregulation of mRNA of interleukin-1 and -6 in subchondral cystic lesions of four horses.
Equine Vet J, 33(2), 143-149.
https://doi.org/10.1111/j.2042-3306.2001.tb00592.x Publication
Researcher Affiliations
- Department of Veterinary Surgery, University of Zürich, Switzerland.
MeSH Terms
- Animals
- Bone Cysts / metabolism
- Bone Cysts / veterinary
- DNA Primers
- Horse Diseases / genetics
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- In Situ Hybridization / veterinary
- Interleukin-1 / genetics
- Interleukin-1 / metabolism
- Interleukin-6 / genetics
- Interleukin-6 / metabolism
- Metacarpus
- Metatarsal Bones
- Polymerase Chain Reaction / veterinary
- RNA, Messenger / metabolism
- Up-Regulation
Citations
This article has been cited 11 times.- Gao L, Cucchiarini M, Madry H. Cyst formation in the subchondral bone following cartilage repair. Clin Transl Med 2020 Dec;10(8):e248.
- Golonka P, Kornicka-Garbowska K, Marycz K. SIRT1(+) Adipose Derived Mesenchymal Stromal Stem Cells (ASCs) Suspended in Alginate Hydrogel for the Treatment of Subchondral Bone Cyst in Medial Femoral Condyle in the Horse. Clinical Report. Stem Cell Rev Rep 2020 Dec;16(6):1328-1334.
- Green CJ, Beck A, Wood D, Zheng MH. The biology and clinical evidence of microfracture in hip preservation surgery. J Hip Preserv Surg 2016 Jul;3(2):108-23.
- Stübinger S, Nuss K, Bürki A, Mosch I, le Sidler M, Meikle ST, von Rechenberg B, Santin M. Osseointegration of titanium implants functionalised with phosphoserine-tethered poly(epsilon-lysine) dendrons: a comparative study with traditional surface treatments in sheep. J Mater Sci Mater Med 2015 Feb;26(2):87.
- Inui A, Nakano S, Yoshioka S, Goto T, Hamada D, Kawasaki Y, Egawa H, Yasui N. Subchondral cysts in dysplastic osteoarthritic hips communicate with the joint space: analysis using three-dimensional computed tomography. Eur J Orthop Surg Traumatol 2013 Oct;23(7):791-5.
- Plecko M, Sievert C, Andermatt D, Frigg R, Kronen P, Klein K, Stübinger S, Nuss K, Bürki A, Ferguson S, Stoeckle U, von Rechenberg B. Osseointegration and biocompatibility of different metal implants--a comparative experimental investigation in sheep. BMC Musculoskelet Disord 2012 Mar 8;13:32.
- Beekman L, Tohver T, Dardari R, Léguillette R. Evaluation of suitable reference genes for gene expression studies in bronchoalveolar lavage cells from horses with inflammatory airway disease. BMC Mol Biol 2011 Jan 28;12:5.
- Bogaert L, Van Poucke M, De Baere C, Peelman L, Gasthuys F, Martens A. Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids. BMC Biotechnol 2006 Apr 27;6:24.
- Akens MK, von Rechenberg B, Bittmann P, Nadler D, Zlinszky K, Auer JA. Long term in-vivo studies of a photo-oxidized bovine osteochondral transplant in sheep. BMC Musculoskelet Disord 2001;2:9.
- Ramsaran LN, Byron M, Parry S, Lection J, Back B, Grenier J, Cheong SH, Diel de Amorim M. Investigation of gene stability in equine luteal tissue during mid-diestrus phase and early pregnancy - Research Article. BMC Vet Res 2026 Jan 9;22(1):84.
- Pérez-Nogués M, López-Sanromán J, Spirito M, Manso-Díaz G. Treatments and Prognosis for Subchondral Cystic Lesions in the Distal Extremities in Thoroughbred Prospect Racehorses. Animals (Basel) 2023 Sep 7;13(18).
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