Use of a protected catheter brush for culture of the lower respiratory tract in horses with small airway disease.
Abstract: A protected catheter brush introduced by fiberoptic bronchoscopy was used to sample the tracheai and bronchial mucosa in 28 horses with small airway disease. Tracheal and bronchial brushings were examined for the presence of fungi, aerobic and anaerobic bacteria, and a cytoiogical evaluation was also done on fluid collected by the bronchoalveolar lavage (BAL) technique. Microorganisms (bacteria and fungi) were isolated more often in tracheal brushings (53.6%) than in bronchial brushings (10.7%). Anaerobic bacteria were not isolated. Results of this study indicate that fiberoptic bronchoscopy using a protected catheter brush is an easy and practical technique to obtain minimally contaminated samples for isolation of microorganisms from the lower respiratory tract of horses. However, no association was observed between isolation of high numbers of microorganisms from the bronchi and severity of small airway disease.
Publication Date: 1991-01-01 PubMed ID: 1884285PubMed Central: PMC1263413
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study explores the use of a protected catheter brush to collect samples from the lower respiratory tracts of horses who have small airway disease. The researchers found it an effective way to gather minimally contaminated samples but found no correlation between a large number of microorganisms in the bronchi and the severity of small airway disease.
Research Methodology
- The study uses a protected catheter brush introduced via fiberoptic bronchoscopy to take samples from the tracheal and bronchial mucosa in 28 horses with small airway disease.
- The researchers conducted a cytological evaluation on fluid collected by the bronchoalveolar lavage (BAL) technique to assess the presence of cells and other substances.
Key Findings
- Microorganisms; specifically bacteria and fungi were discovered more often in tracheal brushings, at a rate of 53.6%, in comparison to bronchial brushings, at a rate of 10.7%.
- No anaerobic bacteria were isolated in the samples.
- Use of a protected catheter brush was found to be a practical and efficient technique for gathering minimally contaminated samples from the lower respiratory tracts of horses.
- There was no connection found between the isolation of microorganisms in high numbers from the bronchi and the severity of the small airway disease.
Conclusion
- The study indicates that utilizing protected catheter brushes through fiberoptic bronchoscopy is a useful and feasible method to secure minimally contaminated samples from the lower respiratory tract of horses suffering small airway disease.
- The technique, however, doesn’t validate the hypothesis that there is an association between the severity of small airway disease and the volume of microorganisms isolated from the bronchi.
Cite This Article
APA
Grandguillot L, Fairbrother JM, Vrins A.
(1991).
Use of a protected catheter brush for culture of the lower respiratory tract in horses with small airway disease.
Can J Vet Res, 55(1), 50-55.
Publication
Researcher Affiliations
- Departement de pathologie et de microbiologie, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
MeSH Terms
- Animals
- Bacteria / isolation & purification
- Bronchi / microbiology
- Bronchoalveolar Lavage Fluid / cytology
- Bronchoalveolar Lavage Fluid / microbiology
- Bronchoscopy
- Cell Count / veterinary
- Fiber Optic Technology
- Fungi / isolation & purification
- Horse Diseases / microbiology
- Horses
- Lung Diseases, Obstructive / microbiology
- Lung Diseases, Obstructive / veterinary
- Specimen Handling / veterinary
- Trachea / microbiology
References
This article includes 25 references
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Citations
This article has been cited 2 times.- Kinoshita Y, Niwa H, Katayama Y, Hariu K. Dominant obligate anaerobes revealed in lower respiratory tract infection in horses by 16S rRNA gene sequencing. J Vet Med Sci 2014 Apr;76(4):587-91.
- Labonville M, Higgins R, Lavoie JP. [Comparison of direct smear observations and bacteriologic culture results carried out on equine tracheal secretions]. Can Vet J 2001 Aug;42(8):623-6.
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