Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon.
Abstract: To determine efficacy of an extracorporeal circuit to maintain a segment of equine large colon for 3.5 hours and to evaluate the effect of low arterial flow on histologic and metabolic variables. Methods: Segments of large colon from 15 healthy adult horses. Methods: The pelvic flexure was surgically removed and maintained in an isolated circuit. In the control group, tissue was evaluated for 3.5 hours, whereas in the low-flow group, arterial flow was reduced to 20% of baseline for 40 minutes followed by 2 hours of reperfusion. Various metabolic and hemodynamic variables were evaluated at 30-minute intervals. Effects of nitric oxide (NO) and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity were determined, and histomorphologic evaluation was performed at the completion of the study. Results: Low-flow ischemia with reperfusion caused significant histomorphologic differences, compared with the control group. In the low-flow group, significant differences included reduction in PaCO2, reduction in bicarbonate concentrations, increase in PaO2, and an increase in base deficit in arterial and venous blood samples. Other significant differences included increases in PCV, protein concentration, total WBC count, and albumin clearance for the low-flow group. Differences were not detected in inhibitory activity of the low-flow group relative to the control tissue with or without addition of NO and L-NAME. Conclusions: The extracorporeal circuit maintained a segment of equine intestine for 3.5 hours and can be used to simulate ischemic injury. The extracorporeal circuit provides the potential to investigate pharmaceutic agents that can minimize intestinal injury.
Publication Date: 2000-09-08 PubMed ID: 10976734DOI: 10.2460/ajvr.2000.61.1042Google Scholar: Lookup
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- Clinical Trial
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the use of an extracorporeal circuit to maintain a part of a horse’s large intestine (colon) for a prolonged period, and evaluates the effects of reduced arterial flow, such as ischemic injury, on various metabolic and histological variables.
Objective and Methods of the Study
- The primary goal of this project was to assess the effectiveness of an extracorporeal circuit in preserving a segment of the equine large colon for a duration of 3.5 hours.
- The researchers also sought to gauge the results of low arterial flow on both histologic (structural) and metabolic attributes. To accomplish this, they reduced arterial flow to 20% of its baseline for a duration of 40 minutes, followed by two hours of reperfusion (restoring the blood supply).
- The pelvic flexure, a section of the large colon, was surgically removed from 15 healthy adult horses and maintained in an isolated circuit for this experiment.
Results and Findings of the Study
- Comparing the control group with the low-flow group, the research revealed substantial histomorphologic differences due to low-flow ischemia and reperfusion.
- Among other observable changes, the low-flow group showed a decrease in PaCO2 and bicarbonate concentrations, an increase in PaO2, and an increased base deficit in arterial and venous blood samples.
- Other significant changes noted were higher PCV, protein concentration, total white blood cell (WBC) count, and albumin clearance in the low-flow group.
- However, the research detected no significant differences in inhibitory activity between the low-flow group and the control tissue when treated with or without nitric oxide (NO) and L-N-nitro-arginine-methyl-ester (L-NAME).
Conclusions of the Study
- This research verified that an extracorporeal circuit can effectively maintain a segment of equine intestine for up to 3.5 hours and can be employed for simulating ischemic injury.
- Furthermore, it offered the potential to research pharmaceutical agents that could mitigate intestinal damage.
Cite This Article
APA
Van Hoogmoed LM, Snyder JR, Nieto JE, Vatistas NJ, Harmon FA.
(2000).
Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon.
Am J Vet Res, 61(9), 1042-1051.
https://doi.org/10.2460/ajvr.2000.61.1042 Publication
Researcher Affiliations
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
MeSH Terms
- Animals
- Blood Gas Analysis / veterinary
- Carbon Dioxide / blood
- Colon / blood supply
- Extracorporeal Circulation / veterinary
- Female
- Horse Diseases / physiopathology
- Horses
- Intestinal Mucosa / metabolism
- Ischemia / physiopathology
- Ischemia / veterinary
- Leukocyte Count
- Male
- Muscle Contraction / drug effects
- NG-Nitroarginine Methyl Ester / pharmacology
- Nitric Oxide / pharmacology
- Oxygen / blood
- Reperfusion Injury / veterinary
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