Use of an isolated intestinal circuit to evaluate the effect of ischemia and reperfusion on mucosal permeability of the equine jejunum.
Abstract: To evaluate the efficacy of an isolated perfusion circuit and the effect of ischemia-reperfusion on mucosal permeability of the jejunum. Methods: In vitro study of intestinal mucosal permeability. Methods: Twelve healthy adult horses. Methods: A control segment of jejunum was placed in an isolated perfusion circuit for 240 minutes and mucosal permeability was measured. After detecting no deleterious effects of the isolated system on the control intestine, low flow ischemia was created in experimental segments for 20, 40, 60 and 90 minutes followed by 60 minutes of reperfusion and mucosal permeability was evaluated. At the completion of the studies, histologic evaluation was used to determine mucosal grades, surface area, and volume. Results: Control tissue was maintained in the isolated circuit for 240 minutes without effect on mucosal grade, surface area, or volume relative to intact tissue. After ischemia-reperfusion, mucosal grade increased, and volume and surface area decreased progressively with longer periods of ischemia. Mucosal clearance of albumin remained constant during 240 minutes of perfusion in control tissue and was elevated after ischemia-reperfusion. Conclusions: No deleterious changes were noted in jejunum perfused with this isolated circuit, whereas alterations in mucosal permeability were present after ischemia-reperfusion. Conclusions: The isolated perfusion circuit successfully maintained an isolated segment of jejunum within physiologic limits, and can be used to evaluate the effects of injury and the efficacy of pharmaceuticals to attenuate these changes.
Copyright 2003 by The American College of Veterinary Surgeons
Publication Date: 2003-01-10 PubMed ID: 12520490DOI: 10.1053/jvet.2003.49999Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research is a study examining whether an isolated perfusion circuit can effectively maintain a segment of horse intestine (jejunum) and the impact of blood supply cut-off and re-establishment (ischemia-reperfusion) on jejunum’s mucosal permeability.
Methodology
- The study was conducted in an in vitro setup using the intestines of twelve healthy adult horses.
- A control segment of the jejunum was placed in an isolated perfusion circuit for 240 minutes and the permeability of the mucosa, the innermost layer of the intestine, was measured.
- After confirming that the isolated system had no harmful effects on the control intestine, low flow ischemia (restriction of blood supply to the tissues) was induced in experimental segments for 20, 40, 60, and 90 minutes. This was followed by 60 minutes of reperfusion (restoration of blood flow) and the mucosal permeability was evaluated again.
- At the end of the experiment, histologic evaluation, an examination of the microscopic structure of tissues, was used to assess mucosal grades, surface area, and volume.
Results
- The research found that the isolated circuit managed to maintain the control tissue for a duration of 240 minutes without any effect on the mucosal grading, surface area, or volume relative to intact tissue.
- Following ischemia-reperfusion, there were observed increases in mucosal grading, and decreases in volume and surface area, which worsened with longer periods of ischemia.
- The mucosal clearance of albumin, a protein whose rate of passage through the mucosal barrier can indicate its permeability, remained constant during the 240 minutes of perfusion in control tissue but was found to be increased after ischemia-reperfusion.
Conclusion
- The research concluded that no harmful changes were observed in the jejunum when perfused with the isolated circuit.
- However, alterations in the permeability of the mucosa were observed after ischemia-reperfusion.
- The isolated perfusion circuit was successful in maintaining an isolated segment of the jejunum within physiological limits and can be used to study the effects of injury and to gauge the efficacy of drugs in mitigating these changes.
Cite This Article
APA
Vatistas NJ, Nieto JE, Van Hoogmoed L, Gardner I, Snyder JR.
(2003).
Use of an isolated intestinal circuit to evaluate the effect of ischemia and reperfusion on mucosal permeability of the equine jejunum.
Vet Surg, 32(1), 52-61.
https://doi.org/10.1053/jvet.2003.49999 Publication
Researcher Affiliations
- Comparative Gastroenterology Laboratory, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
MeSH Terms
- Albumins / pharmacokinetics
- Animals
- Blood Flow Velocity
- Extracorporeal Circulation / veterinary
- Horse Diseases / physiopathology
- Horses / physiology
- Intestinal Mucosa / blood supply
- Ischemia / physiopathology
- Ischemia / veterinary
- Jejunal Diseases / physiopathology
- Jejunal Diseases / veterinary
- Jejunum / blood supply
- Muscle Contraction
- Reperfusion / veterinary
Citations
This article has been cited 4 times.- Unterköfler MS, McGorum BC, Milne EM, Licka TF. Establishment of a model for equine small intestinal disease: effects of extracorporeal blood perfusion of equine ileum on metabolic variables and histological morphology - an experimental ex vivo study. BMC Vet Res 2019 Nov 8;15(1):400.
- Demirkan A, Orazakunov E, Savaş B, Kuzu MA, Melli M. Enteral glutamine pretreatment does not decrease plasma endotoxin level induced by ischemia-reperfusion injury in rats. World J Gastroenterol 2008 Jan 21;14(3):463-8.
- Delesalle C, Deprez P, Schuurkes JA, Lefebvre RA. Contractile effects of 5-hydroxytryptamine and 5-carboxamidotryptamine in the equine jejunum. Br J Pharmacol 2006 Jan;147(1):23-35.
- Ludwig EK, Abraham N, Schaaf CR, McKinney CA, Freund J, Stewart AS, Veerasammy BA, Thomas M, Cardona DM, Garman K, Barbas AS, Sudan DL, Gonzalez LM. Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability. Am J Transplant 2024 Apr;24(4):564-576.
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