Use of buserelin to induce ovulation in the cyclic mare.
Abstract: Inducing ovulation in a cyclic mare is often necessary. For this purpose, hCG has been used commonly, but the response can be reduced after successive administrations. The aims of this study were to test the effectiveness of buserelin in hastening ovulation in estrus mares, and its influence on fertility; and to investigate the effect of treatment on LH secretion. Five crossover trials were designed to compare the effect of two treatments: buserelin (40 microg in 4 doses i.v. at 12 h intervals) vs placebo (Experiments 1 and 2); buserelin 40 microg (in 4 doses i.v.) vs 20 microg (Experiment 3); buserelin (4 doses of 20 microg i.v.) vs hCG (1 dose of 2,500 IU i.v.) (Experiment 4); or buserelin (3 doses of 13.3 microg at 6 h interval) vs hCG (Experiment 5). In Experiment 2, blood samples were taken hourly until ovulation, for LH measurements. In Experiment 1, buserelin treatment significantly hastened ovulation. Reduction of the dose by half (Experiment 3) did not alter the effectiveness. In Experiments 4 and 5, buserelin was as effective as hCG in inducing ovulation between 24 and 48 h after initiation of treatment. Buserelin treatment induced a rise in LH concentration during the 48 h period of the experiment, and LH concentrations before ovulation were significantly higher in buserelin treated cycles than in placebo cycles. These experiments demonstrated the usefulness of two new protocols of administration of buserelin, as an alternative to hCG for induction of ovulation. One hypothesis explaining the mechanism of action is that the persistant rise in LH concentration could modify the ratio of biological/immunological LH, as it occurs physiologically, thereby hastening ovulation.
Publication Date: 2001-06-08 PubMed ID: 11393219DOI: 10.1016/s0093-691x(01)00512-xGoogle Scholar: Lookup
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- Clinical Trial
- Comparative Study
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the efficacy of buserelin, a hormone, in speeding up ovulation in cyclic mares, and its effect on their fertility. It compares the use of buserelin with that of the traditionally used hormone, hCG, and explores its impact on LH secretion.
Objective and Methodology
- The objective of this study was to assess the effectiveness of buserelin in inducing ovulation faster in estrus mares and its influence on their fertility. The study also aimed to examine the treatment’s impact on Luteinizing Hormone (LH) secretion.
- Five experiments were designed to evaluate these effects, with each comparing two treatments: buserelin at different doses versus a placebo, buserelin versus a smaller dose, and buserelin versus hCG. These provided a wide range of comparative scenarios for assessing the efficacy of buserelin.
Results and Findings
- Experiment 1 showed that the buserelin treatment notably expedited ovulation. Experiment 3 concluded that reducing the administration dose by half didn’t alter the effectiveness of buserelin.
- Experiments 4 and 5 demonstrated that buserelin was as efficient as hCG in inducing ovulation within 24 to 48 hours following the beginning of treatment. This reveals buserelin’s potential to be a viable alternative to hCG in hastening ovulation.
- It was observed that buserelin treatment induced an increase in LH concentration during the 48-hour period of the experiment, and the LH levels prior to ovulation were significantly higher in buserelin-treated cycles compared to placebo cycles.
Conclusion
- The research concluded the effectiveness of buserelin as an alternative for hCG for inducing ovulation in cyclic mares. It also provided a potential explanation for buserelin’s mechanism of action: it could modify the ratio of biological to immunological LH in a way that occurs naturally, thereby accelerating ovulation.
Cite This Article
APA
Barrier-Battut I, Le Poutre N, Trocherie E, Hecht S, Grandchamp des Raux A, Nicaise JL, Vérin X, Bertrand J, Fiéni F, Hoier R, Renault A, Egron L, Tainturier D, Bruyas JF.
(2001).
Use of buserelin to induce ovulation in the cyclic mare.
Theriogenology, 55(8), 1679-1695.
https://doi.org/10.1016/s0093-691x(01)00512-x Publication
Researcher Affiliations
- Department of Reproduction, Veterinary School, Nantes, France.
MeSH Terms
- Animals
- Breeding / methods
- Buserelin / administration & dosage
- Buserelin / pharmacology
- Chorionic Gonadotropin / pharmacology
- Cross-Over Studies
- Drug Administration Schedule / veterinary
- Female
- Fertility Agents, Female / administration & dosage
- Fertility Agents, Female / pharmacology
- Horses / physiology
- Luteinizing Hormone / blood
- Ovulation Induction / methods
- Time Factors
Citations
This article has been cited 2 times.- Bottrel M, Ortiz I, Hidalgo M, Díaz-Jiménez M, Pereira B, Consuegra C, Yousef MS, Dorado J. Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate. Animals (Basel) 2022 Jan 8;12(2).
- Miki W, Oniyama H, Takeda N, Kimura Y, Haneda S, Matsui M, Taya K, Nambo Y. Effects of a single use of the GnRH analog buserelin on the induction of ovulation and endocrine profiles in heavy draft mares. J Equine Sci 2016;27(4):149-156.
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