Use of immunohistochemical methods for diagnosis of equine pythiosis.
Abstract: Excisional biopsy specimens were received from 8 horses with lesions suspected of being caused by Pythium sp. Pythium sp was isolated from all specimens. An indirect peroxidase technique was applied to formalin-fixed tissues from the biopsy material and there was distinct staining of hyphal cell walls in all specimens. Sections containing hyphae, other than those of Pythium sp, failed to stain. The indirect peroxidase technique could be used for confirmation of suspected cases of pythiosis.
Publication Date: 1988-11-01 PubMed ID: 3073678
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research focused on using immunohistochemical methods, specifically an indirect peroxidase technique, for diagnosing equine pythiosis, a disease in horses caused by the Pythium sp fungus. The study found this technique to be an effective way to confirm suspected cases of pythiosis.
Study Objective and Methodology
- The research aimed to investigate the efficacy of immunohistochemical methods, particularly the indirect peroxidase technique, in diagnosing equine pythiosis.
- The method applied was experimental, testing biopsy samples taken from eight horses showing symptoms assumed to be resulting from a Pythium sp infection.
- The samples were prepared through a formalin-fixation process and then subjected to the indirect peroxidase technique. This process involves the addition of peroxidase to enzyme immunoassay procedures, which react with chromogenic substrates to produce visible staining under a microscope.
Key Results of the Study
- Through this study, it was confirmed that Pythium sp was indeed present in all collected samples.
- All prepared tissue samples that underwent the indirect peroxidase technique showed distinct staining of the hyphal cell walls. Hyphae are the filamentous cells of fungi, like Pythium sp, which are responsible for nutrient absorption and growth.
- The indirect peroxidase technique successfully differentiated the Pythium sp hyphae from other types as non-Pythium sp hyphae failed to stain. This demonstrates the specificity of the technique for Pythium sp.
Conclusion from the Study
- The study concluded that the indirect peroxidase technique is an effective tool for confirming suspected cases of equine pythiosis, due to its ability to distinctively stain Pythium sp hyphae.
- The use of this immunohistochemical approach could provide a valuable diagnostic tool for equine veterinarians and improve the accuracy and speed of pythiosis diagnosis in horses.
Cite This Article
APA
Brown CC, McClure JJ, Triche P, Crowder C.
(1988).
Use of immunohistochemical methods for diagnosis of equine pythiosis.
Am J Vet Res, 49(11), 1866-1868.
Publication
Researcher Affiliations
- Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
MeSH Terms
- Animals
- Biopsy / veterinary
- Chytridiomycota / isolation & purification
- Dermatomycoses / diagnosis
- Dermatomycoses / microbiology
- Dermatomycoses / veterinary
- Female
- Horse Diseases / diagnosis
- Horse Diseases / microbiology
- Horses
- Immunoenzyme Techniques
- Immunohistochemistry
- Male
- Pythium / isolation & purification
Citations
This article has been cited 10 times.- White AG, Smart K, Hathcock T, Tillson DM, Poudel A, Rynders P, Wang C. Successful management of cutaneous paralagenidiosis in a dog treated with mefenoxam, minocycline, prednisone, and hyperbaric oxygen therapy.. Med Mycol Case Rep 2020 Sep;29:38-42.
- Chitasombat MN, Jongkhajornpong P, Lekhanont K, Krajaejun T. Recent update in diagnosis and treatment of human pythiosis.. PeerJ 2020;8:e8555.
- Inkomlue R, Larbcharoensub N, Karnsombut P, Lerksuthirat T, Aroonroch R, Lohnoo T, Yingyong W, Santanirand P, Sansopha L, Krajaejun T. Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis.. J Clin Microbiol 2016 Jan;54(1):43-8.
- Dória RG, Carvalho MB, Freitas SH, Laskoski LM, Colodel EM, Mendonça FS, Silva MA, Grigoletto R, Fantinato Neto P. Evaluation of intravenous regional perfusion with amphotericin B and dimethylsulfoxide to treat horses for pythiosis of a limb.. BMC Vet Res 2015 Jul 16;11:152.
- Pereira DI, Botton SA, Azevedo MI, Motta MA, Lobo RR, Soares MP, Fonseca AO, Jesus FP, Alves SH, Santurio JM. Canine gastrointestinal pythiosis treatment by combined antifungal and immunotherapy and review of published studies.. Mycopathologia 2013 Oct;176(3-4):309-15.
- Pereira DI, Schild AL, Motta MA, Fighera RA, Sallis ES, Marcolongo-Pereira C. Cutaneous and gastrointestinal pythiosis in a dog in Brazil.. Vet Res Commun 2010 Mar;34(3):301-6.
- Krajaejun T, Keeratijarut A, Sriwanichrak K, Lowhnoo T, Rujirawat T, Petchthong T, Yingyong W, Kalambaheti T, Smittipat N, Juthayothin T, Sullivan TD. The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.. Clin Vaccine Immunol 2010 Aug;17(8):1203-10.
- Chindamporn A, Vilela R, Hoag KA, Mendoza L. Antibodies in the sera of host species with pythiosis recognize a variety of unique immunogens in geographically divergent Pythium insidiosum strains.. Clin Vaccine Immunol 2009 Mar;16(3):330-6.
- Schurko AM, Mendoza L, de Cock AW, Bedard JE, Klassen GR. Development of a species-specific probe for Pythium insidiosum and the diagnosis of pythiosis.. J Clin Microbiol 2004 Jun;42(6):2411-8.
- Mendoza L, Kaufman L, Mandy W, Glass R. Serodiagnosis of human and animal pythiosis using an enzyme-linked immunosorbent assay.. Clin Diagn Lab Immunol 1997 Nov;4(6):715-8.
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