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Equine veterinary journal2014; 47(4); 478-488; doi: 10.1111/evj.12283

Use of laser capture microdissection for the assessment of equine lamellar basal epithelial cell signalling in the early stages of laminitis.

Abstract: Dysadhesion of laminar basal epithelial cells (LBECs) from the underlying dermis is the central event leading to structural failure in equine laminitis. Although many studies of sepsis-related laminitis have reported multiple events occurring throughout the lamellar tissue, there is minimal information regarding signalling events occurring specifically in LBECs. Objective: To determine signalling events in the LBECs during the early stages of carbohydrate-induced laminitis. Methods: Experimental study. Methods: Eight horses were given an overload of carbohydrate (CHO) consisting of corn starch mixture via nasogastric tube. Prior to administration of CHO, lamellar biopsies were taken from the left forefoot (control [CON]). Biopsies were taken from the left hind foot at the onset of fever (developmental [DEV]) and from the right forefoot at the onset of Obel grade 1 lameness (OG1). Laminar basal epithelial cells were isolated from cryosections using a laser capture microdissection (LCM) microscope. Next generation sequencing (RNA-seq) was used to identify transcripts expressed in the LBECs for each time point and bioinformatic analysis was performed with thresholds for between group comparisons set at a greater than 2-fold change and P value ≤0.05. Results: Forty genes (22 increased/18 decreased) were significantly different from DEV time vs. CON and 107 genes (57 increased/50 decreased) were significantly different from OG1 time vs. CON. Significant increases in inflammatory genes were present in addition to significantly altered expression of genes related to extracellular matrix composition, stability and turnover. Conclusions: Signalling related to inflammatory response and extracellular matrix regulation was strongly represented at the DEV and OG1 times. These results indicate that the LBEC is not only a casualty but also an active participant in lamellar events leading to structural failure of the digital lamellae in equine laminitis.
© 2014 EVJ Ltd.
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Publication Date: 2014-08-19 PubMed ID: 24750316PubMed Central: PMC4771185DOI: 10.1111/evj.12283Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is a study on the role of the equine laminar basal epithelial cells (LBECs) during the early stages of laminitis, an inflammatory condition in horses, particularly looking into their signalling events when laminitis is induced by a carbohydrate overload.

Objective & Methodology of the Study

  • The study aims to determine specific signalling events in the LBECs during the early stages of laminitis induced by an overload of carbohydrates. The primary focus is on understanding the factors that lead to the dysadhesion of LBECs from the underlying dermis, a central event in equine laminitis.
  • For the study, eight horses were given an overload of a corn starch mixture via a nasogastric tube. Lamellar biopsies were taken before the administration of the carbohydrate overload, at the onset of the fever, and at the beginning of Obel grade 1 lameness.
  • The LBECs were isolated from the cryosections using a laser capture microdissection (LCM) microscope, and next-generation sequencing (RNA-seq) was employed to identify transcripts expressed in the LBECs at each time point. Bioinformatic analysis was performed with significant differences identified as a more than 2-fold change with a P value ≤0.05.

Findings of the Study

  • The analysis found that 40 genes (22 increased/18 decreased) were significantly different from the fever onset vs control, and 107 genes (57 increased/50 decreased) were significantly different from the onset of Obel grade 1 lameness vs control.
  • The study discovered significant increases in inflammatory genes, along with significantly altered expression of genes related to extracellular matrix composition, stability, and turnover.

Conclusions

  • The research concluded that signalling related to the inflammatory response and extracellular matrix regulation was strongly represented in the LBECs during the early stage of laminitis.
  • It also challenged the prevailing notion that the LBECs are merely victims but suggests that they are active participants in the lamellar events leading to the structural failure of the digital lamellae in equine laminitis.

Cite This Article

APA
Leise BS, Watts MR, Roy S, Yilmaz AS, Alder H, Belknap JK. (2014). Use of laser capture microdissection for the assessment of equine lamellar basal epithelial cell signalling in the early stages of laminitis. Equine Vet J, 47(4), 478-488. https://doi.org/10.1111/evj.12283

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 47
Issue: 4
Pages: 478-488

Researcher Affiliations

Leise, B S
  • Department of Clinical Sciences, College of Veterinary and Biomedical Sciences, Colorado State University, Fort Collins, USA.
Watts, M R
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus, USA.
Roy, S
  • Department of Surgery, College of Medicine, Ohio State University, Columbus, USA.
Yilmaz, A S
  • Biomedical Informatics Shared Resource, Ohio State University Comprehensive Cancer Center, Columbus, USA.
Alder, H
  • Biomedical Informatics Shared Resource, Ohio State University Comprehensive Cancer Center, Columbus, USA.
Belknap, J K
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus, USA.

MeSH Terms

  • Animals
  • Carbohydrates / toxicity
  • Epithelial Cells / cytology
  • Epithelial Cells / physiology
  • Foot Diseases / chemically induced
  • Foot Diseases / physiopathology
  • Foot Diseases / veterinary
  • Gene Expression Regulation
  • Hoof and Claw
  • Horse Diseases / chemically induced
  • Horse Diseases / metabolism
  • Horse Diseases / physiopathology
  • Horses
  • Inflammation / chemically induced
  • Inflammation / veterinary
  • Laser Capture Microdissection / veterinary
  • RNA / genetics
  • RNA / metabolism
  • Transcriptome

Grant Funding

  • UL1 TR001070 / NCATS NIH HHS
  • UL1 TR000090 / NCATS NIH HHS
  • UL1TR000090 / NCATS NIH HHS
  • R01 NR015676 / NINR NIH HHS
  • S10 RR031859 / NCRR NIH HHS
  • R01 DK076566 / NIDDK NIH HHS

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Citations

This article has been cited 8 times.
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