Use of matrix metalloproteinases 2 and 9 and white blood cell counts in monitoring the treatment and predicting the survival of horses with septic arthritis.

The research article investigates the role of matrix metalloproteinases 2 and 9 along with white blood cell counts in predicting the survival outcomes and aiding the treatment monitoring of horses with septic arthritis. The findings suggest these parameters can serve as good prognostic indicators.

Objective of the Study

• The main objective of this study was to explore the use of matrix metalloproteinases (MMPs) 2 and 9 and white blood cell counts (WBCC) in prognosticating survival and assisting treatment monitoring in horses afflicted with septic arthritis.

Methodology of the Study

• The study was conducted on 32 horses with suspected septic arthritis, from which 39 samples of synovial fluid were taken.
• Simultaneously, 39 samples were collected from horses that were euthanased due to non-orthopaedic conditions, to serve as controls.
• The WBCC in each sample was determined, and both the pro (inactive precursor) and active forms of MMPs 2 and 9 were gauged by a method known as gelatin zymography, followed by image analysis.

Key Findings of the Study

• The research found that the initial measurements of the proMMP9:proMMP2 ratio and the WBCC were effective in indicating prognosis of survival in horses suffering from septic arthritis. This provided an insight into the potential use of these parameters in predicting outcomes for affected horses.
• Interestingly, no substantial relationship was observed between the time interval from when the injury occurred to when the horse was referred for treatment and either the WBCC or levels of MMP2 and MMP9 initially.
• Moreover, the research presented no evidence to suggest that this referred interval significantly influenced the survival chances of the horses, indicating that the prognostic value of the MMPs and WBCC was independent of the stage of condition at the time of measurement.