Uterine clinical findings, fertility rate, leucocyte migration, and COX-2 protein levels in the endometrial tissue of susceptible mares treated with platelet-rich plasma before and after AI.
Abstract: Persistent mating-induced endometritis (PMIE) results in decreased fertility in horses, thereby causing a significant impact in the horse market. Platelet-rich plasma (PRP), a modulator of the inflammatory response, has been largely used in veterinary medicine. Here, we investigated the effects of PRP on uterine inflammation, conception rate, endometrial polymorphonuclear neutrophil (PMN) migration, and COX-2 protein levels in the endometrial tissue. Thirteen PMIE-susceptible mares were used for artificial insemination (AI). The mares were inseminated with fresh semen in three consecutive cycles in a cross-over study design. The following cycle classifications were used: control cycle, no pharmacological interference; pre-AI, 20 mL of PRP was infused 24 h before AI; and post-AI, 20 mL of PRP was infused four h after AI. Follicular dynamics were monitored daily by transrectal ultrasound. When a follicle larger than 35 mm was detected, ovulation was induced with deslorelin acetate (1 mg, im). AI was performed 24 h after ovulation induction. Intrauterine fluid (FLU) was evaluated by ultrasonography before and 24 h after AI. PMNs in uterine cytology (CYT) and biopsy (HIS) were also observed before and 24 h after AI. Pregnancy was determined within 14 days after ovulation. Number of COX-2 positive cells was evaluated by immunohistochemistry. Both PRP treatments resulted in a decrease of PMNs in the CYT after breeding when compared to controls. FLU did not differ between cycles; however, the conception rates were significantly higher in the PRP mares. Mares positive for endometritis decreased in both treatment groups, and a more intense positive COX-2 labeling was observed in the control group when compared to the two treatment groups. In conclusion, PRP beneficially reduces inflammatory response in PMIE mares independent of when treatments were administered, thus increasing chances of successful pregnancy.
Copyright © 2017 Elsevier Inc. All rights reserved.
Publication Date: 2017-08-12 PubMed ID: 28822903DOI: 10.1016/j.theriogenology.2017.08.007Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research highlights the effects of Platelet-rich plasma (PRP) treatment in mares affected by Persistent mating-induced endometritis (PMIE), a condition that facilitates reduced fertility. The PRP treatment was observed to reduce uterine inflammation and enhance the conception rate in horses.
Research Methodology
- This research utilized 13 mares identified as PMIE-susceptible for artificial insemination (AI). These mares were inseminated in three consecutive cycles using a cross-over study design.
- The study was divided into three cycles. In the control cycle, no medicinal interference was introduced. For the pre-AI cycle, the researchers infused the mares with 20 mL of PRP 24 hours before artificial insemination. For the post-AI cycle, another 20 mL of PRP was infused four hours following AI.
- The dynamics of follicles in the treated mares were carefully monitored on a day-to-day basis with the help of transrectal ultrasound. Upon detection of follicles greater than 35 mm, researchers induced ovulation using deslorelin acetate. Artificial insemination was then performed 24 hours post ovulation induction.
- The researchers evaluated intrauterine fluids by means of ultrasonography both before and 24 hours after AI. Endometrial polymorphonuclear neutrophil (PMN) migration was examined both prior to and 24 hours post-AI.
- To establish a rate of pregnancy, the mares were monitored for 14 days after ovulation. The presence of COX-2 positive cells was estimated by immunohistochemistry.
Findings of the Study
- The findings showed that both treatments of PRP led to a decrease in PMNs in the uterine cytology after breeding, when compared to the control groups.
- Intrauterine fluid levels were largely similar across all cycles, however, mares treated with PRP featured significantly higher conception rates.
- The number of mares testing positive for endometritis dropped in both the pre-AI and post-AI groups.
- There was notably more intense positive COX-2 labeling in the control group when contrasted against the two treatment groups.
Conclusion
- The study concludes that PRP successfully reduces the inflammatory response in PMIE-affected mares, irrespective of the timing of treatment administration, thus improving chances of successful pregnancy.
Cite This Article
APA
Segabinazzi LG, Friso AM, Correal SB, Crespilho AM, Dell'Aqua JA, Miró J, Papa FO, Alvarenga MA.
(2017).
Uterine clinical findings, fertility rate, leucocyte migration, and COX-2 protein levels in the endometrial tissue of susceptible mares treated with platelet-rich plasma before and after AI.
Theriogenology, 104, 120-126.
https://doi.org/10.1016/j.theriogenology.2017.08.007 Publication
Researcher Affiliations
- Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
- Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
- Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain.
- Santo Amaro University, UNISA, São Paulo, Brazil; Severino Sombra University, Vassouras, Rio de Janeiro, Brazil.
- Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
- Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain.
- Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
- Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil. Electronic address: malvarenga@fmvz.unesp.br.
MeSH Terms
- Animals
- Cell Movement / physiology
- Endometriosis / prevention & control
- Endometriosis / veterinary
- Endometrium / drug effects
- Endometrium / metabolism
- Female
- Horse Diseases / prevention & control
- Horses / physiology
- Insemination, Artificial / veterinary
- Leukocytes / drug effects
- Leukocytes / physiology
- Platelet-Rich Plasma
- Pregnancy
- Pregnancy Rate
Citations
This article has been cited 12 times.- Novello G, Souza FF, Canisso IF. Platelet-Rich Plasma Proteome of Mares Susceptible to Persistent-Breeding-Induced Endometritis Differs from Resistant Mares. Animals (Basel) 2024 Jul 18;14(14).
- Anitua E, Allende M, de la Fuente M, Del Fabbro M, Alkhraisat MH. Efficacy of Platelet-Rich Plasma in Women with a History of Embryo Transfer Failure: A Systematic Review and Meta-Analysis with Trial Sequential Analysis. Bioengineering (Basel) 2023 Feb 27;10(3).
- Morrell JM, Rocha A. A Novel Approach to Minimising Acute Equine Endometritis That May Help to Prevent the Development of the Chronic State. Front Vet Sci 2021;8:799619.
- Lin Y, Qi J, Sun Y. Platelet-Rich Plasma as a Potential New Strategy in the Endometrium Treatment in Assisted Reproductive Technology. Front Endocrinol (Lausanne) 2021;12:707584.
- Fantini P, Jiménez R, Vilés K, Iborra A, Palhares MS, Catalán J, Prades M, Miró J. Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis. Animals (Basel) 2021 Oct 9;11(10).
- Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
- Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel) 2021 Apr 23;10(5).
- Dawod A, Miro J, Elbaz HT, Fahmy H, Abdoon AS. Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GF(equina)) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares. Animals (Basel) 2021 Apr 14;11(4).
- Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
- Kusumi M, Ihana T, Kurosawa T, Ohashi Y, Tsutsumi O. Intrauterine administration of platelet-rich plasma improves embryo implantation by increasing the endometrial thickness in women with repeated implantation failure: A single-arm self-controlled trial. Reprod Med Biol 2020 Oct;19(4):350-356.
- Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology. Int J Mol Sci 2020 Feb 20;21(4).
- Zhang S, Li P, Yuan Z, Tan J. Platelet-rich plasma improves therapeutic effects of menstrual blood-derived stromal cells in rat model of intrauterine adhesion. Stem Cell Res Ther 2019 Feb 15;10(1):61.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
