Validation and usefulness of the Sperm Quality Analyzer V equine for equine semen analysis.
Abstract: Routine semen analysis includes evaluation of concentration combined with seminal volume, morphology and motility. Subjective analysis of these parameters is known to be inaccurate, imprecise and subject to variability. Automated semen analysis could lead to an increased standardization in and between laboratories but for that to happen automated devices need to be validated. A new device, the sperm quality analyzer V equine (SQA-Ve) version 1.00.43, was evaluated for its repeatability and agreement with light microscopy (LM), for raw and extended equine semen. Results were compared with computer assisted sperm analysis (CASA), which was also tested for its repeatability and agreement with LM. The SQA-Ve showed a good repeatability and fine agreement for assessing sperm concentration of raw semen based on scatter and Bland-Altman plots. This was in contrast with the motility parameters, which had a low repeatability. Morphology assessment with SQA-Ve was poorly repeatable as well as in poor agreement with LM. For extended semen, the findings were comparable. The SQA-Ve did well for concentration, whereas for the motility parameters repeatability was only just acceptable, with no agreement with LM. This sharply contrasted the CASA findings that were highly repeatable and almost in perfect agreement with LM. Based on these findings, the tested version of the SQA-Ve is insufficiently accurate to be used for analyzing raw or extended equine semen.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication Date: 2010-10-20 PubMed ID: 20965556DOI: 10.1016/j.theriogenology.2010.08.003Google Scholar: Lookup
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- Journal Article
- Validation Study
Summary
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This research study evaluates the effectiveness of a new automated device, the Sperm Quality Analyzer V equine (SQA-Ve), in the analysis of equine semen, comparing its repeatability and agreement with light microscopy and with computer assisted sperm analysis (CASA). The study concludes that while the SQA-Ve showed high repeatability and agreement in assessing sperm concentration, this was not the case with motility parameters and morphology assessment, especially when compared to CASA. The researchers found that the tested version of the SQA-Ve was less accurate for analysis of raw or extended equine semen.
Study Methods and Parameters
- The researchers focused on routine parameters of semen analysis, including semen concentration, seminal volume, morphology (structure), and motility (movement).
- They compared the results of the ‘SQA-Ve’ device with both light microscopy (LM) and computer assisted sperm analysis (CASA), which were also examined for their repeatability and agreement with LM.
Evaluation of SQA-Ve Device
- For raw equine semen, the SQA-Ve showed reliable repeatability and fine agreement with LM in assessing sperm concentration, as determined by scatter and Bland-Altman plots.
- However, the SQA-Ve displayed poor repeatability and agreement with LM for assessing parameters of motility and morphology of raw semen.
- When analyzing extended semen, the results were the same. Again, the device delivered accurate assessments for concentration, but its performance fell short while measuring motility.
Comparison with CASA
- In contrast to the results from the SQA-Ve, the CASA findings showed highly repeatable results and were almost in perfect agreement with LM.
- This means that compared to CASA, the SQA-Ve lagged behind as a reliable tool for assessing raw or extended equine semen.
Conclusions of the Study
- Based on the obtained results, the tested version of the SQA-Ve was not sufficiently accurate for routine semen analysis in comparison to the established method, and therefore needs improvement before it can be recommended for use in laboratories analyzing equine semen.
Cite This Article
APA
Hoogewijs M, De Vliegher S, De Schauwer C, Govaere J, Smits K, Hoflack G, de Kruif A, Van Soom A.
(2010).
Validation and usefulness of the Sperm Quality Analyzer V equine for equine semen analysis.
Theriogenology, 75(1), 189-194.
https://doi.org/10.1016/j.theriogenology.2010.08.003 Publication
Researcher Affiliations
- Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. maarten.hoogewijs@UGent.be
MeSH Terms
- Animals
- Horses
- Male
- Reproducibility of Results
- Semen Analysis / methods
- Semen Analysis / veterinary
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 2 times.- Sugihara K, Shibata TK, Takata K, Kimura T, Kanayama N, Williams R, Hatakeyama S, Akama TO, Kuo CW, Khoo KH, Fukuda MN. Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans. FEBS Lett 2013 Oct 1;587(19):3195-201.
- Egyptien S, Deleuze S, Ledeck J, Ponthier J. Sperm Quality Assessment in Stallions: How to Choose Relevant Assays to Answer Clinical Questions. Animals (Basel) 2023 Oct 6;13(19).
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