Validation of a transendoscopic glandular and nonglandular gastric biopsy technique in horses.
Abstract: In order to study the evolution of histopathological and immunohistochemical changes in the gastric mucosa of horses with EGUS (equine gastric ulcer syndrome), a feasible, useful, valid and safe in vivo gastric biopsy technique is required. Objective: To determine the average gastric mucosal healing time following endoscopic gastric biopsy sampling, and evaluate the feasibility, safety and usefulness of samples obtained by this method for histopathological analysis. Methods: Six mature mares from the Faculté de Médecine Vétérinaire research herd were used. Transendoscopic gastric biopsy was performed on Days 0 and 9 using a flexible forceps with oval and fenestrated jaws to obtain gastric mucosal samples from 4 different sites: cardia (C), fundus (F), margo plicatus (MP) and glandular mucosa (GL). A maximum of 4 samples per site was taken and processed routinely for histopathology, evaluated by a pathologist. On Days 1-4 and 9-11 the lesions created by the biopsies were evaluated by gastroscopy. Lesions were evaluated over time based on a score from 0-4, where 4 was the most severe. Results: Biopsy samples could be obtained from all targeted sites except C. No abnormal clinical signs were observed up to 7 days post biopsy. The average biopsy lesion scores decreased significantly with time for all sites. The average lesion score was significantly higher for the MP compared to the other sites at Days 1 and 2. Samples taken from the nonglandular portion of the stomach were considered inadequate for histopathology, while those taken from the glandular mucosa were adequate. Conclusions: The transendoscopic gastric biopsy technique described here is a feasible, safe and useful technique for obtaining samples from the equine gastric glandular mucosa. Although biopsy samples could be obtained from several areas in the nonglandular mucosa, these were very small, took longer to heal and were not considered adequate for histopathological evaluation, and another technique should therefore be validated.
Publication Date: 2009-11-26 PubMed ID: 19927580DOI: 10.2746/042516409x424144Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates a transendoscopic biopsy technique used on horses to study histopathological and immunohistochemical changes in their gastric mucosa. The research focuses on studying gastric mucosal healing time after biopsy and assesses the feasibility, safety, and usefulness of sample gathering via this method for analysis purposes.
Methodology
- The study included six mature mares from the Faculté de Médecine Vétérinaire research herd.
- A transendoscopic gastric biopsy was performed on the horses twice, on Days 0 and 9. This procedure was used to gather gastric mucosal samples from four different sites within the horse’s stomach.
- A maximum of four samples was taken from each site and sent for routine processing and analysis by a pathologist.
- The lesions created by the biopsies were assessed via gastroscopy on Days 1-4 and Days 9-11. These lesions were scored from 0-4, with 4 being the most severe.
Results
- All intended biopsy sites were successfully sampled apart from the cardia (C).
- No abnormal clinical signs were noticed until seven days after the biopsy.
- The lesion scores decreased significantly over time for all biopsy sites.
- Samples from the nonglandular portion of the stomach were considered inadequate for histopathological analysis, while those taken from the glandular mucosa were deemed adequate.
Conclusions
- The transendoscopic gastric biopsy technique successfully obtained samples from the equine gastric glandular mucosa, showing that it’s a feasible, safe technique for such purposes.
- Even though biopsy samples could be taken from several areas in the nonglandular mucosa, these samples were very small and required more time for healing. Moreover, they were not sufficient for histopathological evaluation, indicating a need for the validation of other techniques.
Cite This Article
APA
Rodrigues NL, Doré M, Doucet MY.
(2009).
Validation of a transendoscopic glandular and nonglandular gastric biopsy technique in horses.
Equine Vet J, 41(7), 631-635.
https://doi.org/10.2746/042516409x424144 Publication
Researcher Affiliations
- Département de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
MeSH Terms
- Animals
- Biopsy / adverse effects
- Biopsy / methods
- Biopsy / veterinary
- Endoscopy, Gastrointestinal / adverse effects
- Endoscopy, Gastrointestinal / methods
- Endoscopy, Gastrointestinal / veterinary
- Female
- Gastric Mucosa / pathology
- Horse Diseases / diagnosis
- Horses
- Reproducibility of Results
- Wound Healing
Citations
This article has been cited 2 times.- Tesena P, Korchunjit W, Taylor J, Wongtawan T. Comparison of commercial RNA extraction kits and qPCR master mixes for studying gene expression in small biopsy tissue samples from the equine gastric epithelium.. J Equine Sci 2017;28(4):135-141.
- Niedźwiedź A, Kubiak K, Nicpoń J. Endoscopic findings of the stomach in pleasure horses in Poland.. Acta Vet Scand 2013 Jun 7;55(1):45.
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