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Home / Equine Research Bank / Anim Genet / Validation of microsatellite markers for routine horse paren...
Animal genetics1997; 28(4); 247-252; doi: 10.1111/j.1365-2052.1997.00123.x

Validation of microsatellite markers for routine horse parentage testing.

Abstract: A parallel testing of 4803 routine Quarter Horse parentage cases, using 15 loci of blood group and protein polymorphisms (blood typing) and 11 loci of dinucleotide repeat microsatellites (DNA typing), validated DNA markers for horse pedigree verification. For the 26 loci, taken together, the theoretical effectiveness of detecting incorrect parentage was 99.999%, making it extremely unlikely that false parentage would fail to be recognized. The tests identified incorrect parentage assignment for 95 offspring (2% of cases). Despite fewer loci, DNA typing was as effective as blood typing and, in parentage exclusion cases, provided more systems to substantiate the genetic incompatibility. Five offspring presented potential genetic incompatibilities with their parents in only a single microsatellite system, but the parentage exclusions could not be confirmed with discordant results at additional loci. Two of these five incompatibilities could be explained as consequences of a null allele and three as fragment size increases or decreases (putative mutations). Provided that an exclusion assignment was based on at least two systems of genetic incompatibility, such rare genetic events did not lead to false exclusions. Notwithstanding the near 100% effectiveness estimations for either typing panel alone to identify incorrect parentage, this validation test showed an actual effectiveness of 97.3% for blood typing and 98.2% for DNA typing. The DNA-based test, however, may feasibly achieve higher efficacy than reported here by adding selected systems to the parentage test panel.
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Publication Date: 1997-08-01 PubMed ID: 9345720DOI: 10.1111/j.1365-2052.1997.00123.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research validates the use of DNA markers for accurate horse pedigree verification, revealing that these markers can be as effective as blood typing methods. The study tests these markers on 4803 Quarter Horse parentage cases, identifying incorrect parentage assignment in approximately 2% of cases.

Research Methodology

  • The research team conducted parallel testing of a large sample size consisting of 4803 routine Quarter Horse parentage cases.
  • The testing involved two kinds of genetic markers: 15 loci of blood group and protein polymorphisms (blood typing) and 11 loci of dinucleotide repeat microsatellites (DNA typing).

Validation of DNA Markers

  • The DNA markers used in this research were validated for horse pedigree verification.
  • The combined effectiveness of detecting incorrect parentage for the 26 loci was estimated at 99.999%. This makes it highly improbable for false parentage to go unrecognized.

Comparative Efficacy

  • The study found DNA typing to be as effective as the established practice of blood typing, despite the latter involving more loci.
  • Moreover, DNA typing provided more systems to substantiate genetic incompatibility in cases where parentage was disputed.

Parentage Assignment Findings

  • The tests identified incorrect parentage assignment for 95 offspring, which translates to about 2% of the total cases.
  • Five offspring presented potential genetic incompatibilities with their parents in only one microsatellite system. However, the parentage exclusions couldn’t be confirmed as there were inconsistent results at additional loci.

Loci Inconsistencies and Rare Genetic Events

  • Out of the five inconsistencies, two could be explained as consequences of a null allele and three as changes in fragment size (potential mutations).
  • If an exclusion assignment was based on at least two systems of genetic incompatibility, such rare genetic events would not lead to false exclusions.

Estimated vs Actual Effectiveness

  • Despite the near 100% theoretical estimations for either typing panel to identify incorrect parentage alone, the validation test showed an actual effectiveness of 97.3% for blood typing and 98.2% for DNA typing.
  • However, the DNA-based test could potentially achieve higher efficacy than reported in this study by selecting specific systems for the parentage test panel.

Cite This Article

APA
Bowling AT, Eggleston-Stott ML, Byrns G, Clark RS, Dileanis S, Wictum E. (1997). Validation of microsatellite markers for routine horse parentage testing. Anim Genet, 28(4), 247-252. https://doi.org/10.1111/j.1365-2052.1997.00123.x

Publication

Animal genetics
ISSN: 0268-9146
NlmUniqueID: 8605704
Country: England
Language: English
Volume: 28
Issue: 4
Pages: 247-252

Researcher Affiliations

Bowling, A T
  • Veterinary Genetics Laboratory, University of California, Davis 95616, USA.
Eggleston-Stott, M L
    Byrns, G
      Clark, R S
        Dileanis, S
          Wictum, E

            MeSH Terms

            • Alleles
            • Animals
            • Blood Grouping and Crossmatching
            • Female
            • Genetic Techniques / veterinary
            • Horses / blood
            • Horses / genetics
            • Male
            • Microsatellite Repeats
            • Pedigree
            • Reproducibility of Results

            Citations

            This article has been cited 22 times.
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