Validation of nonradioactive chemiluminescent immunoassay methods for the analysis of thyroxine and cortisol in blood samples obtained from dogs, cats, and horses.
Abstract: The performances of a radioimmunoassay method, a chemiluminescent immunoassay method, and a chemiluminescent-enzyme immunoassay method were evaluated for the analysis of cortisol and total thyroxine in blood samples obtained from dogs, cats, horses, and humans (reference samples). The analysis of cortisol in human and animal samples exhibited good precision, linearity, and recovery. The 3 methods gave comparable values for the ACTH-induced increase and the dexamethasone-induced decrease in cortisol concentrations in animal samples. The recoveries of total thyroxine from human samples, analyzed by the 3 methods, were comparable. However, the basal total thyroxine concentrations determined by the chemiluminescent immunoassay method were 30-40% lower than those determined by the radioimmunoassay and the chemiluminescent-enzyme immunoassay methods in animal samples. In both human and animal samples, the plot of thyroxine values obtained by the radioimmunoassay method against those obtained by the chemiluminescent immunoassay method or the chemiluminescent-enzyme immunoassay method was linear. However, although the slope of the radioimmunoassay versus chemiluminescent-enzyme immunoassay curve was close to unity, the slope of the radioimmunoassay versus chemiluminescent immunoassay curve was 0.6. This result suggests that, compared with the radioimmunoassay method, the chemiluminescent immunoassay method underestimated thyroxine values in animal samples but not in human samples. Although all 3 methods yielded comparable changes in thyroxine concentrations in response to thyroid stimulating hormone, they did not yield comparable thyroxine concentrations in response to T3 suppression in dogs and cats.
Publication Date: 1997-07-01 PubMed ID: 9249165DOI: 10.1177/104063879700900307Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study compares the effectiveness of three methods for measuring amounts of cortisol and thyroxine in blood samples from pets and livestock animals. The results suggest that all three methods provide accurate measurements for cortisol, but one method may underestimate levels of thyroxine in animal samples.
Evaluation of Three Immunoassay Methods
- The research team aimed to compare the effectiveness of three different immunoassay methods, which are laboratory techniques used to measure the presence and quantity of specific substances in blood samples. These methods were radioimmunoassay, chemiluminescent immunoassay, and chemiluminescent-enzyme immunoassay.
Investigating Cortisol Levels
- The first part of the study focused on analysing cortisol, a hormone that helps regulate stress responses in many organisms including humans and animals.
- The tested animals include dogs, cats, horses, and the reference samples were from human beings.
- The results showed that each of the three methods performed well in regard to precision, linearity, and recovery of cortisol in both human and animal samples.
Examining Thyroxine Measurements
- The study also investigated measurements of total thyroxine, a hormone produced by the thyroid gland that regulates metabolism.
- When thyroxine was analysed in human samples using each of the three methods, results demonstrated similar recoveries. However, in animal blood samples, the measurements using the chemiluminescent immunoassay method were 30-40% lower than those using the other two methods.
- Even though chemiluminescent immunoassay method showed reliable linear relation with the radioimmunoassay and the chemiluminescent-enzyme immunoassay methods in both human and animal samples, it seemed to underestimate thyroxine values in animal samples, compared to the radioimmunoassay method.
Responses to Thyroid Stimulation
- The study further evaluated how the three methods responded to changes in thyroxine concentrations caused by external influences such as thyroid stimulating hormone and T3 suppression.
- All three methods showed comparable changes when thyroxine levels were shifted due to thyroid stimulating hormone.
- However, the tested methods didn’t yield consistent concentrations results under T3 suppression in dogs and cats.
Cite This Article
APA
Singh AK, Jiang Y, White T, Spassova D.
(1997).
Validation of nonradioactive chemiluminescent immunoassay methods for the analysis of thyroxine and cortisol in blood samples obtained from dogs, cats, and horses.
J Vet Diagn Invest, 9(3), 261-268.
https://doi.org/10.1177/104063879700900307 Publication
Researcher Affiliations
- Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
MeSH Terms
- Adrenocorticotropic Hormone / pharmacology
- Animals
- Cats
- Dexamethasone / pharmacology
- Dogs
- Female
- Horses
- Humans
- Hydrocortisone / blood
- Immunoassay / methods
- Immunoenzyme Techniques
- Luminescent Measurements
- Male
- Radioimmunoassay / methods
- Reference Values
- Regression Analysis
- Reproducibility of Results
- Sensitivity and Specificity
- Thyrotropin / pharmacology
- Thyroxine / blood
- Triiodothyronine / pharmacology
Citations
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