Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. II. Differential leukocyte counts.
Abstract: The Sysmex XT-2000iV is a laser-based, flow cytometric hematology system that stains nucleic acids in leukocytes with a fluorescent dye. A 4-part differential is obtained using side fluorescence light and laser side scatter. Objective: The purpose of this study was to validate the Sysmex XT-2000iV for determining differential leukocyte counts in blood from ill dogs, cats, and horses. Methods: Blood samples from diseased animals (133 dogs, 65 cats, and 73 horses) were analyzed with the Sysmex XT-2000iV (Auto-diff) and the CELL-DYN 3500. Manual differentials were obtained by counting 100 leukocytes in Wright-stained blood smears. Results: Leukocyte populations in the Sysmex DIFF scattergram were usually well separated in equine samples, but were not as well separated in canine and feline samples. Correlation among the Sysmex XT-2000iV, CELL-DYN 3500, and manual counts was excellent for neutrophil counts (r >or=.97) and good for lymphocyte counts (r >or=.87) for all three species. Systematic differences between the 3 methods were seen for lymphocyte and monocyte counts. The Sysmex reported incomplete differential counts on 18% of feline, 13% of canine, and 3% of equine samples, often when a marked left shift (>10% bands) and/or toxic neutrophils were present. Eosinophils were readily identified in cytograms from all 3 species. Neither the Sysmex nor the CELL-DYN detected basophils in the 7 dogs and 5 cats with basophilia. Conclusions: The Sysmex XT-2000iV automated differential leukocyte count performed well with most samples from diseased dogs, cats, and horses. Basophils were not detected. Immature neutrophils or prominent toxic changes often induced errors in samples from cats and dogs.
Publication Date: 2009-04-06 PubMed ID: 19392762DOI: 10.1111/j.1939-165X.2009.00126.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article examines and validates the effectiveness of the Sysmex XT-2000iV hematology system in determining differential leukocyte counts in ill dogs, cats, and horses, focusing primarily on its performance and accuracy compared to other lab methods.
Objective and Methodology
- The primary goal of this study was to validate the efficacy of the Sysmex XT-2000iV hematology system in assessing differential leukocyte counts in the blood of sick dogs, cats, and horses.
- For their methods, the researchers gathered blood samples from the ill animals, which included 133 dogs, 65 cats, and 73 horses.
- These samples were then analyzed utilizing the Sysmex XT-2000iV system (referred to as Auto-diff), as well as the CELL-DYN 3500. Manual differentials were also obtained by counting 100 leukocytes in Wright-stained blood smears.
Results
- The results indicated that leukocyte populations in the Sysmex DIFF scattergram were well-distinguished in equine samples, yet, the separation wasn’t as evident in feline and canine samples.
- Excellent correlation was found between the Sysmex XT-2000iV, CELL-DYN 3500, and manual counts for neutrophil counts (r >or=.97) and pretty good for lymphocyte counts (r >or=.87) for all three species.
- However, systematic differences among the three methodologies were noticed for lymphocyte and monocyte counts.
- The study also found that Sysmex reported incomplete differential counts on significant portions of feline, canine, and equine samples, particularly when a marked left shift (>10% bands) and/or toxic neutrophils were present.
- Eosinophils were readily identified in cytograms from all three species. However, neither the Sysmex nor the CELL-DYN were able to detect basophils in dogs and cats diagnosed with basophilia.
Conclusions
- The conclusions of the study suggest that the Sysmex XT-2000iV automated differential leukocyte count functioned well with most of the samples from ill dogs, cats, and horses.
- However, basophils were not detected by the system.
- Cases that involve immature neutrophils or prominent toxic changes often induced errors in the samples, especially those from dogs and cats.
Cite This Article
APA
Lilliehöök I, Tvedten H.
(2009).
Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. II. Differential leukocyte counts.
Vet Clin Pathol, 38(2), 175-182.
https://doi.org/10.1111/j.1939-165X.2009.00126.x Publication
Researcher Affiliations
- University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden. inger.lilliehook@uds.slu.se
MeSH Terms
- Animals
- Cats / blood
- Dogs / blood
- Horses / blood
- Leukocyte Count / instrumentation
- Leukocyte Count / veterinary
- Lymphocyte Subsets
- Reproducibility of Results
- Sensitivity and Specificity
- Species Specificity
Citations
This article has been cited 17 times.- Grebert M, Granat F, Braun JP, Leroy Q, Bourgès-Abella N, Trumel C. Validation of the Sysmex XN-V hematology analyzer for canine specimens. Vet Clin Pathol 2021 Jun;50(2):184-197.
- Oikonomidis IL, Milne E, Piccinelli C. Differential white blood cell counts in rabbits: a comparison of the Advia 2120 and a manual method. J Vet Diagn Invest 2021 Jul;33(4):670-676.
- Dondi F, Vasylyeva K, Serafini F, Gruarin M, Troìa R, Giunti M, Agnoli C. Heinz body-related interference with leukocyte and erythrocyte variables obtained by an automated hematology analyzer in cats. J Vet Diagn Invest 2019 Sep;31(5):704-713.
- Herman N, Trumel C, Geffré A, Braun JP, Thibault M, Schelcher F, Bourgès-Abella N. Hematology reference intervals for adult cows in France using the Sysmex XT-2000iV analyzer. J Vet Diagn Invest 2018 Sep;30(5):678-687.
- Balan M, Hope A, Cassidy J, McCullough M, O'Brien PJ. Marked paraneoplastic basophilia accompanying eosinophilia in a cat with alimentary T-cell lymphoma. JFMS Open Rep 2017 Jul-Dec;3(2):2055116917730180.
- Tvedten HW, Andersson V, Lilliehöök IE. Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil-Lymphocyte Error and How to Avoid It. J Vet Intern Med 2017 Nov;31(6):1708-1716.
- Brandeker E, Hillström A, Hanås S, Hagman R, Holst BS. The effect of a single dose of prednisolone in dogs envenomated by Vipera berus--a randomized, double-blind, placebo-controlled clinical trial. BMC Vet Res 2015 Feb 26;11:44.
- Rossi G, Cerquetella M, Antonelli E, Pengo G, Magi GE, Villanacci V, Rostami-Nejad M, Spaterna A, Bassotti G. The importance of histologic parameters of lacteal involvement in cases of canine lymphoplasmacytic enteritis. Gastroenterol Hepatol Bed Bench 2015 Winter;8(1):33-41.
- Stirn M, Moritz A, Bauer N. Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms. BMC Vet Res 2014 Jun 5;10:125.
- Martínez-Caro J, Pastor J. Characterization of Feline Basophils on the Sysmex XN-1000V and Evaluation of a New WDF Gating Profile. Animals (Basel) 2024 Nov 22;14(23).
- Mau A, Keller SM, Kol A. A clinical and hematologic approach to basophilia in dogs, cats, and horses. Vet Clin Pathol 2025 Mar;54(1):24-34.
- Rong N, Wu J, Zhao B, Peng W, Yang H, Zhang G, Ruan D, Wei X, Liu J. Comparison of the pathogenicity and neutrophil and monocyte response between SARS-CoV-2 prototype and Omicron BA.1 in a lethal mouse model. Animal Model Exp Med 2025 Apr;8(4):707-717.
- Zelmer KC, Moritz A, Bauer N. Evaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method. J Vet Diagn Invest 2023 Nov;35(6):679-697.
- Yoshida T, Uemura A, Tanaka R, Farag A, Mandour AS, Hamabe L, Matsumoto K. Secondary right atrial thrombosis in three dogs: Antithrombotics therapy and echocardiographic follow-up. Vet Med Sci 2023 Sep;9(5):1973-1979.
- Spada E, Antognoni MT, Proverbio D, Ferro E, Mangili V, Miglio A. Haematological and biochemical reference intervals in adult Maine Coon cat blood donors. J Feline Med Surg 2015 Dec;17(12):1020-7.
- Granat F, Geffré A, Bourgès-Abella N, Mortier J, Théron ML, Fauchon E, Braun JP, Trumel C. Feline reference intervals for the Sysmex XT-2000iV and the ProCyte DX haematology analysers in EDTA and CTAD blood specimens. J Feline Med Surg 2014 Jun;16(6):473-82.
- Granat F, Geffré A, Bourgès-Abella N, Braun JP, Trumel C. Changes in haematology measurements with the Sysmex XT-2000iV during storage of feline blood sampled in EDTA or EDTA plus CTAD. J Feline Med Surg 2013 Jun;15(6):433-44.
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