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Home / Equine Research Bank / Front Microbiol / vB_PaeM_MIJ3, a Novel Jumbo Phage Infecting Pseudomonas aeru...
Frontiers in microbiology2019; 10; 2772; doi: 10.3389/fmicb.2019.02772

vB_PaeM_MIJ3, a Novel Jumbo Phage Infecting Pseudomonas aeruginosa, Possesses Unusual Genomic Features.

Abstract: Phages are the most abundant biological entity on Earth. There are many variants in phage virion sizes, morphology, and genome sizes. Large virion sized phages, with genome sizes greater than 200 kbp have been identified and termed as Jumbo phages. These phages exhibit certain characteristics that have not been reported in phages with smaller genomes. In this work, a jumbo phage named MIJ3 (vB_PaeM_MIJ3) that infects PAO1 was isolated from an equine livery yard in Leicestershire, United Kingdom. The genome and biological characteristics of this phage have been investigated. MIJ3 is a Myovirus with multiple long tail fibers. Assessment of the host range of MIJ3 revealed that it has the ability to infect many clinical isolates of Bioinformatics analysis of the phage genome indicated that MIJ3 is closely related to the phage, PA5oct. MIJ3 possesses several unusual features that are either rarely present in other phages or have not yet been reported. In particular, MIJ3 encodes a FtsH-like protein, and a putative lysidine synthase, TilS. These two proteins have not been reported in phages. MIJ3 also possesses a split DNA polymerase B with a novel intein. Of particular interest, unlike other jumbo phages infecting spp., MIJ3 lacks the genetic elements required for the formation of the phage nucleus, which was believed to be conserved across jumbo phages.
Copyright © 2019 Imam, Alrashid, Patel, Dowah, Brown, Millard, Clokie and Galyov.
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Publication Date: 2019-11-28 PubMed ID: 31849908PubMed Central: PMC6892783DOI: 10.3389/fmicb.2019.02772Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores a new variant of Jumbo phage, named MIJ3, that infects Pseudomonas aeruginosa. It investigates the phage’s specific features and how it differs from other known Jumbo phages.

Details About The Jumbo Phage MIJ3

  • The research presents a new Jumbo phage known as MIJ3, which has been shown to infect Pseudomonas aeruginosa, a bacteria often associated with diseases in animals and humans.
  • The term ‘Jumbo’ refers to the large size of these phages – they have virion sizes and genomes larger than typical phages.
  • The MIJ3 phage was isolated from an equine livery yard in Leicestershire, United Kingdom.
  • It is classified as a Myovirus characterized by long tail fibers, this structural feature is common among phages that infect a broad range of bacterial hosts.
  • Bioinformatics analysis of the MIJ3 phage genome revealed it is closely related to the Pseudomonas aeruginosa phage, PA5oct.

Unique Traits of the MIJ3 Phage

  • The MIJ3 phage stands out from the crowd due to its unique features, some of which are rarely found in other types of phages, or have never been reported before.
  • Two key Proteins, the FtsH-like protein and a putative lysidine synthase, TilS, encoded by the MIJ3 genome, are the first of their kind to be discovered in phages. These proteins could have implications for the life cycle of the phage or the way it interacts with its host.
  • The MIJ3 possess a split DNA polymerase B with a novel intein. This protein and the particular set up may play a crucial role in DNA recombination and repair mechanisms of the phage.

Differences from Other Jumbo Phages

  • Despite its close relation to other jumbo phages that infect Pseudomonas, a defining characteristic of MIJ3 is its lack of genetic elements required for the formation of the phage nucleus.
  • It was previously believed that these genetic elements were conserved across all jumbo phages, but the existence of the MIJ3 phage presents a deviation from this theory.

Cite This Article

APA
Imam M, Alrashid B, Patel F, Dowah ASA, Brown N, Millard A, Clokie MRJ, Galyov EE. (2019). vB_PaeM_MIJ3, a Novel Jumbo Phage Infecting Pseudomonas aeruginosa, Possesses Unusual Genomic Features. Front Microbiol, 10, 2772. https://doi.org/10.3389/fmicb.2019.02772

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 10
Pages: 2772
PII: 2772

Researcher Affiliations

Imam, Mohammed
  • Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
  • Laboratory Department, University Medical Center, Umm Al-Qura University, Mecca, Saudi Arabia.
Alrashid, Bandar
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
  • King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Patel, Faizal
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
Dowah, Ahmed S A
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
Brown, Nathan
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
Millard, Andrew
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
Clokie, Martha R J
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.
Galyov, Edouard E
  • Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester, Leicester, United Kingdom.

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