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BioMed research international2018; 2018; 8719281; doi: 10.1155/2018/8719281

Viral, Serological, and Antioxidant Investigations of Equine Rhinitis A Virus in Serum and Nasal Swabs of Commercially Used Horses in Poland.

Abstract: Equine rhinitis A virus (ERAV) is considered to be an important pathogen in horses, but relatively few studies are available. Objective: The purpose of this study was to verify ERAV seroprevalence in selected horses in Poland, in addition to correlation between ERAV and age and sex of analysed animals and the antioxidant status. Methods: The material collected from clinically healthy horses was tested using the VNT (353 serum samples) and virus isolation method (44 nasal swabs). 27 serum samples with antibody titers between 0 and ≥1 : 2048 were chosen for further analysis. The study was conducted in group 1 (ERAV titer ≤ 64) and group 2 (ERAV titer > 64). Results: Seroneutralisation tests showed positive results in 72% of serum samples. No significant correlation between ERAV seropositive results and selected biochemical indicators was observed. Group 2 had statistically higher concentrations of SOD and CuZnSOD than the analysed group 1. Conclusions: ERAV was not detected in the nasal swab samples. Antioxidant parameters did not significantly vary between horses of different breed, sex, or age. The ERAV virus had an impact on plasma total SOD and Cu/Zn SOD activity in horses in early stages of convalescence.
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Publication Date: 2018-04-22 PubMed ID: 29850583PubMed Central: PMC5937552DOI: 10.1155/2018/8719281Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the prevalence and characteristics of the Equine Rhinitis A virus (ERAV) in horses in Poland, and explored correlations with the age and sex of the horses, as well as their antioxidant status. The study did not find a significant correlation with age, sex or breed, but did find that the ERAV virus had an impact on certain antioxidant activities in horses during early stages of convalescence.

Objective and Methodology

  • The main aim of the study was to verify the prevalence of ERAV in selected horses in Poland, and to determine any correlations with age, sex, and antioxidant status of these animals.
  • The team collected samples from clinically healthy horses and tested them using the Virus Neutralization Test (VNT) and virus isolation methods. A total of 353 serum samples and 44 nasal swabs were collected for testing.
  • Subsequent analysis was conducted on 27 serum samples which had antibody levels ranging from 0 to and exceeding 1:2048. The study was performed on two groups – Group 1, with ERAV titer less than or equal to 64, and Group 2, with ERAV titer greater than 64.

Results

  • The Virus Neutralization Test found positive results in 72% of the serum samples, suggesting a high prevalence of ERAV in the sampled horse population.
  • Contrary to the expected outcomes, no significant correlation was found between ERAV positive results and selected biochemical indicators in the horses.
  • It was observed that Group 2, the horses with higher ERAV titer, had statistically higher concentrations of antioxidants namely superoxide dismutase (SOD) and copper zinc superoxide dismutase (CuZnSOD) in comparison to Group 1.
  • Interestingly, ERAV was not detected in any of the nasal swab samples collected from the horses, suggesting that the virus may not be present in the nasal passages of the sampled population or the methodology chosen for testing was not sensitive enough.

Conclusions

  • The antioxidant parameters did not significantly vary between horses of different breed, sex, or age, indicating that these parameters do not contribute to a horse’s propensity for ERAV.
  • The research clearly signals that the presence of ERAV has an observable impact on plasma’s total SOD and Cu/Zn SOD activity in horses during early stages of recovery post infection. This implies that a horse’s antioxidant response may be a potential area of focus or marker for ERAV.

Cite This Article

APA
Bażanów B, Frącka A, Jackulak N, Romuk E, Gębarowski T, Owczarek A, Stygar D. (2018). Viral, Serological, and Antioxidant Investigations of Equine Rhinitis A Virus in Serum and Nasal Swabs of Commercially Used Horses in Poland. Biomed Res Int, 2018, 8719281. https://doi.org/10.1155/2018/8719281

Publication

BioMed research international
ISSN: 2314-6141
NlmUniqueID: 101600173
Country: United States
Language: English
Volume: 2018
Pages: 8719281
PII: 8719281

Researcher Affiliations

Bażanów, Barbara
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Frącka, Agnieszka
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Jackulak, Natalia
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Romuk, Ewa
  • Department of Biochemistry, School of Medicine with Dentistry Division in Zabrze, Medical University of Silesia, Katowice, Poland.
Gębarowski, Tomasz
  • Department of Basic Medical Sciences, Wrocław Medical University, Wrocław, Poland.
Owczarek, Aleksander
  • Department of Statistics, Department of Instrumental Analysis, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland.
Stygar, Dominika
  • Chair and Department of Physiology, School of Medicine with Dentistry Division in Zabrze, Medical University of Silesia, Katowice, Poland.

MeSH Terms

  • Animals
  • Antioxidants / metabolism
  • Aphthovirus / physiology
  • Biomarkers / metabolism
  • Cell Line
  • Cytopathogenic Effect, Viral
  • Female
  • Horses / blood
  • Horses / virology
  • Lipid Peroxidation
  • Male
  • Nasal Cavity / virology
  • Oxidation-Reduction
  • Oxidative Stress
  • Poland
  • Rabbits
  • Superoxide Dismutase / blood
  • Virus Replication

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Citations

This article has been cited 3 times.
  1. Bażanów B, Pawęska JT, Pogorzelska A, Florek M, Frącka A, Gębarowski T, Chwirot W, Stygar D. Serological Evidence of Common Equine Viral Infections in a Semi-Isolated, Unvaccinated Population of Hucul Horses.. Animals (Basel) 2021 Jul 30;11(8).
    doi: 10.3390/ani11082261pubmed: 34438717google scholar: lookup
  2. Włoch A, Stygar D, Bahri F, Bażanów B, Kuropka P, Chełmecka E, Pruchnik H, Gładkowski W. Antiproliferative, Antimicrobial and Antiviral Activity of β-Aryl-δ-iodo-γ-lactones, Their Effect on Cellular Oxidative Stress Markers and Biological Membranes.. Biomolecules 2020 Nov 24;10(12).
    doi: 10.3390/biom10121594pubmed: 33255306google scholar: lookup
  3. Bażanów BA, Chełmecka E, Romuk E, Stygar DM. Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses.. Biomed Res Int 2020;2020:7542384.
    doi: 10.1155/2020/7542384pubmed: 32733953google scholar: lookup
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