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BioMed research international2018; 2018; 8719281; doi: 10.1155/2018/8719281

Viral, Serological, and Antioxidant Investigations of Equine Rhinitis A Virus in Serum and Nasal Swabs of Commercially Used Horses in Poland.

Abstract: Equine rhinitis A virus (ERAV) is considered to be an important pathogen in horses, but relatively few studies are available. Objective: The purpose of this study was to verify ERAV seroprevalence in selected horses in Poland, in addition to correlation between ERAV and age and sex of analysed animals and the antioxidant status. Methods: The material collected from clinically healthy horses was tested using the VNT (353 serum samples) and virus isolation method (44 nasal swabs). 27 serum samples with antibody titers between 0 and ≥1 : 2048 were chosen for further analysis. The study was conducted in group 1 (ERAV titer ≤ 64) and group 2 (ERAV titer > 64). Results: Seroneutralisation tests showed positive results in 72% of serum samples. No significant correlation between ERAV seropositive results and selected biochemical indicators was observed. Group 2 had statistically higher concentrations of SOD and CuZnSOD than the analysed group 1. Conclusions: ERAV was not detected in the nasal swab samples. Antioxidant parameters did not significantly vary between horses of different breed, sex, or age. The ERAV virus had an impact on plasma total SOD and Cu/Zn SOD activity in horses in early stages of convalescence.
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Publication Date: 2018-04-22 PubMed ID: 29850583PubMed Central: PMC5937552DOI: 10.1155/2018/8719281Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the prevalence and characteristics of the Equine Rhinitis A virus (ERAV) in horses in Poland, and explored correlations with the age and sex of the horses, as well as their antioxidant status. The study did not find a significant correlation with age, sex or breed, but did find that the ERAV virus had an impact on certain antioxidant activities in horses during early stages of convalescence.

Objective and Methodology

  • The main aim of the study was to verify the prevalence of ERAV in selected horses in Poland, and to determine any correlations with age, sex, and antioxidant status of these animals.
  • The team collected samples from clinically healthy horses and tested them using the Virus Neutralization Test (VNT) and virus isolation methods. A total of 353 serum samples and 44 nasal swabs were collected for testing.
  • Subsequent analysis was conducted on 27 serum samples which had antibody levels ranging from 0 to and exceeding 1:2048. The study was performed on two groups – Group 1, with ERAV titer less than or equal to 64, and Group 2, with ERAV titer greater than 64.

Results

  • The Virus Neutralization Test found positive results in 72% of the serum samples, suggesting a high prevalence of ERAV in the sampled horse population.
  • Contrary to the expected outcomes, no significant correlation was found between ERAV positive results and selected biochemical indicators in the horses.
  • It was observed that Group 2, the horses with higher ERAV titer, had statistically higher concentrations of antioxidants namely superoxide dismutase (SOD) and copper zinc superoxide dismutase (CuZnSOD) in comparison to Group 1.
  • Interestingly, ERAV was not detected in any of the nasal swab samples collected from the horses, suggesting that the virus may not be present in the nasal passages of the sampled population or the methodology chosen for testing was not sensitive enough.

Conclusions

  • The antioxidant parameters did not significantly vary between horses of different breed, sex, or age, indicating that these parameters do not contribute to a horse’s propensity for ERAV.
  • The research clearly signals that the presence of ERAV has an observable impact on plasma’s total SOD and Cu/Zn SOD activity in horses during early stages of recovery post infection. This implies that a horse’s antioxidant response may be a potential area of focus or marker for ERAV.

Cite This Article

APA
Bażanów B, Frącka A, Jackulak N, Romuk E, Gębarowski T, Owczarek A, Stygar D. (2018). Viral, Serological, and Antioxidant Investigations of Equine Rhinitis A Virus in Serum and Nasal Swabs of Commercially Used Horses in Poland. Biomed Res Int, 2018, 8719281. https://doi.org/10.1155/2018/8719281

Publication

BioMed research international
ISSN: 2314-6141
NlmUniqueID: 101600173
Country: United States
Language: English
Volume: 2018
Pages: 8719281
PII: 8719281

Researcher Affiliations

Bażanów, Barbara
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Frącka, Agnieszka
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Jackulak, Natalia
  • Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Romuk, Ewa
  • Department of Biochemistry, School of Medicine with Dentistry Division in Zabrze, Medical University of Silesia, Katowice, Poland.
Gębarowski, Tomasz
  • Department of Basic Medical Sciences, Wrocław Medical University, Wrocław, Poland.
Owczarek, Aleksander
  • Department of Statistics, Department of Instrumental Analysis, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland.
Stygar, Dominika
  • Chair and Department of Physiology, School of Medicine with Dentistry Division in Zabrze, Medical University of Silesia, Katowice, Poland.

MeSH Terms

  • Animals
  • Antioxidants / metabolism
  • Aphthovirus / physiology
  • Biomarkers / metabolism
  • Cell Line
  • Cytopathogenic Effect, Viral
  • Female
  • Horses / blood
  • Horses / virology
  • Lipid Peroxidation
  • Male
  • Nasal Cavity / virology
  • Oxidation-Reduction
  • Oxidative Stress
  • Poland
  • Rabbits
  • Superoxide Dismutase / blood
  • Virus Replication

References

This article includes 36 references
  1. Lynch SE, Gilkerson JR, Symes SJ, Huang JA, Hartley CA. Persistence and chronic urinary shedding of the aphthovirus equine rhinitis A virus.. Comp Immunol Microbiol Infect Dis 2013 Jan;36(1):95-103.
    doi: 10.1016/j.cimid.2012.10.003pubmed: 23183058google scholar: lookup
  2. McCollum WH, Timoney PJ. Studies on the seroprevalence and frequency of equine rhinovirus 1 and 2 infection in normal horse urine. 1992; pp. 83–87.
  3. Horsington J, Lynch SE, Gilkerson JR, Studdert MJ, Hartley CA. Equine picornaviruses: well known but poorly understood.. Vet Microbiol 2013 Nov 29;167(1-2):78-85.
    doi: 10.1016/j.vetmic.2013.05.012pubmed: 23820049google scholar: lookup
  4. Li F, Drummer HE, Ficorilli N, Studdert MJ, Crabb BS. Identification of noncytopathic equine rhinovirus 1 as a cause of acute febrile respiratory disease in horses.. J Clin Microbiol 1997 Apr;35(4):937-43.
    pmc: PMC229704pubmed: 9157156doi: 10.1128/jcm.35.4.937-943.1997google scholar: lookup
  5. Diaz-Méndez A. Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and development of an experimental infection model in horses. .
  6. Mumford JA, Rossdale PD. Virus and its relationship to the "poor performance" syndrome.. Equine Vet J 1980 Jan;12(1):3-9.
    doi: 10.1111/j.2042-3306.1980.tb02285.xpubmed: 6244942google scholar: lookup
  7. Viel L, Diaz-Mendez A. Equine rhinitis viruses: An overlooked cause of respiratory infection. Boehringer Ingelheim Vetmedica Inc., 2013.
  8. Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E. Antiviral effects of pyrrolidine dithiocarbamate on human rhinoviruses.. J Virol 2002 Jun;76(12):6004-15.
    doi: 10.1128/JVI.76.12.6004-6015.2002pmc: PMC136215pubmed: 12021333google scholar: lookup
  9. Paiva CN, Bozza MT. Are reactive oxygen species always detrimental to pathogens?. Antioxid Redox Signal 2014 Feb 20;20(6):1000-37.
    doi: 10.1089/ars.2013.5447pmc: PMC3924804pubmed: 23992156google scholar: lookup
  10. Timoney J, Gillespie JH, Scott FW, Barlough JE. Laboratory diagnosis of viral infection in Hagan and Bruner's microbiology and infectious diseases of domestic animals. 8th. Ithaca, NY, USA: Cornell Univ., Press; 1988.
  11. LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent.. J Biol Chem 1951 Nov;193(1):265-75.
    pubmed: 14907713
  12. Oyanagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity.. Anal Biochem 1984 Nov 1;142(2):290-6.
    doi: 10.1016/0003-2697(84)90467-6pubmed: 6099057google scholar: lookup
  13. Richterich R. Clinical Chemistry. Warsaw, Poland: PZWL; 1971.
  14. Fathiazad F, Khaki A, Nouri M, Khaki AA. Effect of cinnamon zeylanicum on serum testosterone and anti-oxidants levels in rats. International Journal of Women's Health and Reproduction Sciences 2013;1(1):29–35.
    doi: 10.15296/ijwhr.2013.05google scholar: lookup
  15. Erel O. A new automated colorimetric method for measuring total oxidant status.. Clin Biochem 2005 Dec;38(12):1103-11.
    doi: 10.1016/j.clinbiochem.2005.08.008pubmed: 16214125google scholar: lookup
  16. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.. Anal Biochem 1979 Jun;95(2):351-8.
    doi: 10.1016/0003-2697(79)90738-3pubmed: 36810google scholar: lookup
  17. Tsuchida M, Miura T, Mizutani K, Aibara K. Fluorescent substances in mouse and human sera as a parameter of in vivo lipid peroxidation.. Biochim Biophys Acta 1985 Apr 25;834(2):196-204.
    pubmed: 3995061
  18. Burrows R. Laboratory diagnosis of some virus infections of the upper respiratory tract of the horse. Equine Veterinary Journal 1968;1(1):32–38.
    doi: 10.1111/j.2042-3306.1968.tb03342.xgoogle scholar: lookup
  19. Plummer G, Kerry JB. Studies on an equine respiratory virus. Veterinary Record 1962;74:967–970.
  20. Klaey M, Sanchez-Higgins M, Leadon DP, Cullinane A, Straub R, Gerber H. Field case study of equine rhinovirus 1 infection: clinical signs and clinicopathology.. Equine Vet J 1998 May;30(3):267-9.
    doi: 10.1111/j.2042-3306.1998.tb04499.xpubmed: 9622330google scholar: lookup
  21. Kriegshäuser G, Deutz A, Kuechler E, Skern T, Lussy H, Nowotny N. Prevalence of neutralizing antibodies to Equine rhinitis A and B virus in horses and man.. Vet Microbiol 2005 Apr 10;106(3-4):293-6.
    doi: 10.1016/j.vetmic.2004.12.029pubmed: 15778036google scholar: lookup
  22. Plateau E, Levy E. Serological prevalence of equine adenovirus and rhinovirus among horse populations in the district of Paris. Recueil De Medecine Veterinaire 1990;164(4):413–418.
  23. Holmes DF, Kemen MJ, Coggins L. Equine rhinovirus infection - serologic evidence of infection in selected United States horse populations. Equine Infectious Diseases 1978;IV:315–319.
  24. Pagamjav O, Kobayashi K, Murakami H, Tabata Y, Miura Y, Boldbaatar B, Sentsui H. Serological survey of equine viral diseases in Mongolia.. Microbiol Immunol 2011 Apr;55(4):289-92.
    doi: 10.1111/j.1348-0421.2011.00312.xpmc: PMC7158856pubmed: 21447048google scholar: lookup
  25. Gradzki Z, Boguta L, Grądzki Z. Badania serologiczne nad wystepowaniem zakazen wywolywanych przez wirusy zapalenia nosa koni typu b w Polsce. Medycyna Weterynaryjna 2009;65(2):119–123.
  26. Mandas A, Iorio EL, Congiu MG, Balestrieri C, Mereu A, Cau D, Dessì S, Curreli N. Oxidative imbalance in HIV-1 infected patients treated with antiretroviral therapy.. J Biomed Biotechnol 2009;2009:749575.
    doi: 10.1155/2009/749575pmc: PMC2768042pubmed: 19884983google scholar: lookup
  27. Aquaro S, Scopelliti F, Pollicita M, Perno CF. Oxidative stress and HIV infection: Target pathways for novel therapies?. Future HIV Therapy 2008;2(4):327–338.
    doi: 10.2217/17469600.2.4.327google scholar: lookup
  28. Reshi ML, Su YC, Hong JR. RNA Viruses: ROS-Mediated Cell Death.. Int J Cell Biol 2014;2014:467452.
    doi: 10.1155/2014/467452pmc: PMC4034720pubmed: 24899897google scholar: lookup
  29. Kirschvink N, de Moffarts B, Lekeux P. The oxidant/antioxidant equilibrium in horses.. Vet J 2008 Aug;177(2):178-91.
    doi: 10.1016/j.tvjl.2007.07.033pubmed: 17897849google scholar: lookup
  30. Clarkson PM, Thompson HS. Antioxidants: what role do they play in physical activity and health?. Am J Clin Nutr 2000 Aug;72(2 Suppl):637S-46S.
    doi: 10.1093/ajcn/72.2.637Spubmed: 10919970google scholar: lookup
  31. Łuszczewski A, Matyska-Piekarska E, Trefler J, Wawer I, Łacki J, Śliwińska-Stańczyk P. Reactive oxygen species—physiological and pathological function in the human body. Reumatología Clínica 2007;45(5):284–289.
  32. Percival SS, Harris ED. Regulation of Cu,Zn superoxide dismutase with copper. Caeruloplasmin maintains levels of functional enzyme activity during differentiation of K562 cells.. Biochem J 1991 Feb 15;274 ( Pt 1)(Pt 1):153-8.
    doi: 10.1042/bj2740153pmc: PMC1150191pubmed: 1900417google scholar: lookup
  33. Shephard RJ, Shek PN. Cold exposure and immune function.. Can J Physiol Pharmacol 1998 Sep;76(9):828-36.
    doi: 10.1139/y98-097pubmed: 10066131google scholar: lookup
  34. Radakovic M, Davitkov D, Borozan S, Stojanovic S, Stevanovic J, Krstic V, Stanimirovic Z. Oxidative stress and DNA damage in horses naturally infected with Theileria equi.. Vet J 2016 Nov;217:112-118.
    doi: 10.1016/j.tvjl.2016.10.003pubmed: 27810201google scholar: lookup
  35. Marañón G, Muñoz-Escassi B, Manley W, García C, Cayado P, de la Muela MS, Olábarri B, León R, Vara E. The effect of methyl sulphonyl methane supplementation on biomarkers of oxidative stress in sport horses following jumping exercise.. Acta Vet Scand 2008 Nov 7;50(1):45.
    doi: 10.1186/1751-0147-50-45pmc: PMC2586020pubmed: 18992134google scholar: lookup
  36. Onmaz AC, Van Den Hoven R, Gunes V, Cinar M, Kucuk O. Oxidative stress in horses after a 12-hours transport period. Revue de Médecine Vétérinaire 2011;162(4):213–217.

Citations

This article has been cited 4 times.
  1. Bażanów B, Pawęska JT, Pogorzelska A, Florek M, Frącka A, Gębarowski T, Chwirot W, Stygar D. Serological Evidence of Common Equine Viral Infections in a Semi-Isolated, Unvaccinated Population of Hucul Horses. Animals (Basel) 2021 Jul 30;11(8).
    doi: 10.3390/ani11082261pubmed: 34438717google scholar: lookup
  2. Włoch A, Stygar D, Bahri F, Bażanów B, Kuropka P, Chełmecka E, Pruchnik H, Gładkowski W. Antiproliferative, Antimicrobial and Antiviral Activity of β-Aryl-δ-iodo-γ-lactones, Their Effect on Cellular Oxidative Stress Markers and Biological Membranes. Biomolecules 2020 Nov 24;10(12).
    doi: 10.3390/biom10121594pubmed: 33255306google scholar: lookup
  3. Bażanów BA, Chełmecka E, Romuk E, Stygar DM. Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses. Biomed Res Int 2020;2020:7542384.
    doi: 10.1155/2020/7542384pubmed: 32733953google scholar: lookup
  4. Stasiak K, Dunowska M, Rola J. Prevalence and Sequence Analysis of Equine Rhinitis Viruses among Horses in Poland. Viruses 2024 Jul 26;16(8).
    doi: 10.3390/v16081204pubmed: 39205178google scholar: lookup
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