Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare.
Abstract: The complete genome sequence of the type strain Corynebacterium uterequi DSM 45634 from an equine urogenital tract specimen comprises 2,419,437 bp and 2,163 protein-coding genes. Candidate virulence factors are homologs of DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae and of sialidase precursors from Trueperella pyogenes and Chlamydia trachomatis.
Copyright © 2015 Rückert et al.
Publication Date: 2015-07-30 PubMed ID: 26227590PubMed Central: PMC4520889DOI: 10.1128/genomeA.00783-15Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study offers a full genome analysis of a bacterium known as Corynebacterium uterequi, specifically from the type strain DSM 45634, which was taken from a horse’s urogenital tract. The researchers identified potential virulence factors within the identified 2,163 protein-coding genes, including parallels to certain genes in Corynebacterium diphtheriae and precursors to a type of enzyme known as sialidase in other bacteria.
Genome Sequencing and Analysis of Corynebacterium uterequi DSM 45634
In investigating the bacterium Corynebacterium uterequi DSM 45634, which was isolated from a horse’s urogenital tract, the researchers conducted a complete genome sequence. This process essentially provides a full genetic map of the organism, allowing for an in-depth study of its characteristics. The genome of this particular strain was found to comprise 2,419,437 base pairs and 2,163 protein-coding genes, which indicate it carries a significant amount of genetic information.
- This genetic information is vital in understanding the various functions and characteristics of the bacterium, including its ability to survive, replicate, and potentially cause disease within a host organism.
Identification of Virulence Factors
A significant part of the study was dedicated to identifying possible virulence factors within the Corynebacterium uterequi genome. Virulence factors are elements that contribute to an organism’s ability to cause infection or disease.
- In this case, the researchers identified gene homologs comparable to DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae — another species renowned for its pathogenicity, particularly in relation to the respiratory illness diphtheria.
- This finding is significant because it suggests that DSM 45634 may share some pathogenic mechanism with Corynebacterium diphtheriae.
Sialidase Precursor Identification
Sialidase is an enzyme that plays several roles in bacterial pathogenesis, such as promoting adhesion to host tissues and aiding in the evasion of the host’s immune response. Sialidase precursors have been identified in multiple pathogenic organisms, and the researchers have identified similar precursors in the genome of Corynebacterium uterequi.
- These precursors bear resemblance to those found in Trueperella pyogenes and Chlamydia trachomatis, two other known pathogenic microorganisms.
- The presence of these precursors again hints that this particular strain of Corynebacterium uterequi could potentially be pathogenic, and contributes more to our understanding of the virulence of this bacterium.
Cite This Article
APA
Rückert C, Kriete M, Jaenicke S, Winkler A, Tauch A.
(2015).
Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare.
Genome Announc, 3(4), e00783-15.
https://doi.org/10.1128/genomeA.00783-15 Publication
Researcher Affiliations
- Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Bioinformatics Resource Facility (BRF), Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany tauch@cebitec.uni-bielefeld.de.
References
This article includes 17 references
- Hoyles L, Ortman K, Cardew S, Foster G, Rogerson F, Falsen E. Corynebacterium uterequi sp. nov., a non-lipophilic bacterium isolated from urogenital samples from horses.. Vet Microbiol 2013 Aug 30;165(3-4):469-74.
- Kämpfer P, Lodders N, Warfolomeow I, Falsen E, Busse HJ. Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant.. Int J Syst Evol Microbiol 2009 May;59(Pt 5):1112-5.
- Tauch A, Sandbote J. The family Corynebacteriaceae. 2014 p 239–277 In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (ed), The prokaryotes, Actinobacteria, 4th ed Springer Verlag, Berlin, Germany.
- Trost E, Ott L, Schneider J, Schröder J, Jaenicke S, Goesmann A, Husemann P, Stoye J, Dorella FA, Rocha FS, Soares Sde C, D'Afonseca V, Miyoshi A, Ruiz J, Silva A, Azevedo V, Burkovski A, Guiso N, Join-Lambert OF, Kayal S, Tauch A. The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.. BMC Genomics 2010 Dec 30;11:728.
- Trost E, Al-Dilaimi A, Papavasiliou P, Schneider J, Viehoever P, Burkovski A, Soares SC, Almeida SS, Dorella FA, Miyoshi A, Azevedo V, Schneider MP, Silva A, Santos CS, Santos LS, Sabbadini P, Dias AA, Hirata R Jr, Mattos-Guaraldi AL, Tauch A. Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors.. BMC Genomics 2011 Jul 30;12:383.
- Schröder J, Maus I, Meyer K, Wördemann S, Blom J, Jaenicke S, Schneider J, Trost E, Tauch A. Complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen Corynebacterium resistens DSM 45100 isolated from blood samples of a leukemia patient.. BMC Genomics 2012 Apr 23;13:141.
- Gordon D, Green P. Consed: a graphical editor for next-generation sequencing.. Bioinformatics 2013 Nov 15;29(22):2936-7.
- Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification.. BMC Bioinformatics 2010 Mar 8;11:119.
- Markowitz VM, Chen IM, Palaniappan K, Chu K, Szeto E, Pillay M, Ratner A, Huang J, Woyke T, Huntemann M, Anderson I, Billis K, Varghese N, Mavromatis K, Pati A, Ivanova NN, Kyrpides NC. IMG 4 version of the integrated microbial genomes comparative analysis system.. Nucleic Acids Res 2014 Jan;42(Database issue):D560-7.
- Sabbadini PS, Assis MC, Trost E, Gomes DL, Moreira LO, Dos Santos CS, Pereira GA, Nagao PE, Azevedo VA, Hirata Júnior R, Dos Santos AL, Tauch A, Mattos-Guaraldi AL. Corynebacterium diphtheriae 67-72p hemagglutinin, characterized as the protein DIP0733, contributes to invasion and induction of apoptosis in HEp-2 cells.. Microb Pathog 2012 Mar;52(3):165-76.
- Antunes CA, Sanches dos Santos L, Hacker E, Köhler S, Bösl K, Ott L, de Luna Md, Hirata R Jr, Azevedo VA, Mattos-Guaraldi AL, Burkovski A. Characterization of DIP0733, a multi-functional virulence factor of Corynebacterium diphtheriae.. Microbiology (Reading) 2015 Mar;161(Pt 3):639-47.
- Ott L, Höller M, Gerlach RG, Hensel M, Rheinlaender J, Schäffer TE, Burkovski A. Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells.. BMC Microbiol 2010 Jan 5;10:2.
- Kolodkina V, Denisevich T, Titov L. Identification of Corynebacterium diphtheriae gene involved in adherence to epithelial cells.. Infect Genet Evol 2011 Mar;11(2):518-21.
- Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Marchler GH, Song JS, Thanki N, Wang Z, Yamashita RA, Zhang D, Zheng C, Bryant SH. CDD: NCBI's conserved domain database.. Nucleic Acids Res 2015 Jan;43(Database issue):D222-6.
- Jost BH, Songer JG, Billington SJ. Cloning, expression, and characterization of a neuraminidase gene from Arcanobacterium pyogenes.. Infect Immun 2001 Jul;69(7):4430-7.
- Hill C. Virulence or niche factors: what's in a name?. J Bacteriol 2012 Nov;194(21):5725-7.
- Klein C, Troedsson M. Equine pre-implantation conceptuses express neuraminidase 2--a potential mechanism for desialylation of the equine capsule.. Reprod Domest Anim 2012 Jun;47(3):449-54.
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
