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Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare.
Abstract: The complete genome sequence of the type strain Corynebacterium uterequi DSM 45634 from an equine urogenital tract specimen comprises 2,419,437 bp and 2,163 protein-coding genes. Candidate virulence factors are homologs of DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae and of sialidase precursors from Trueperella pyogenes and Chlamydia trachomatis.
Copyright © 2015 Rückert et al.
Publication Date: 2015-07-30 PubMed ID: 26227590PubMed Central: PMC4520889DOI: 10.1128/genomeA.00783-15Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The study offers a full genome analysis of a bacterium known as Corynebacterium uterequi, specifically from the type strain DSM 45634, which was taken from a horse’s urogenital tract. The researchers identified potential virulence factors within the identified 2,163 protein-coding genes, including parallels to certain genes in Corynebacterium diphtheriae and precursors to a type of enzyme known as sialidase in other bacteria.
Genome Sequencing and Analysis of Corynebacterium uterequi DSM 45634
In investigating the bacterium Corynebacterium uterequi DSM 45634, which was isolated from a horse’s urogenital tract, the researchers conducted a complete genome sequence. This process essentially provides a full genetic map of the organism, allowing for an in-depth study of its characteristics. The genome of this particular strain was found to comprise 2,419,437 base pairs and 2,163 protein-coding genes, which indicate it carries a significant amount of genetic information.
- This genetic information is vital in understanding the various functions and characteristics of the bacterium, including its ability to survive, replicate, and potentially cause disease within a host organism.
Identification of Virulence Factors
A significant part of the study was dedicated to identifying possible virulence factors within the Corynebacterium uterequi genome. Virulence factors are elements that contribute to an organism’s ability to cause infection or disease.
- In this case, the researchers identified gene homologs comparable to DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae — another species renowned for its pathogenicity, particularly in relation to the respiratory illness diphtheria.
- This finding is significant because it suggests that DSM 45634 may share some pathogenic mechanism with Corynebacterium diphtheriae.
Sialidase Precursor Identification
Sialidase is an enzyme that plays several roles in bacterial pathogenesis, such as promoting adhesion to host tissues and aiding in the evasion of the host’s immune response. Sialidase precursors have been identified in multiple pathogenic organisms, and the researchers have identified similar precursors in the genome of Corynebacterium uterequi.
- These precursors bear resemblance to those found in Trueperella pyogenes and Chlamydia trachomatis, two other known pathogenic microorganisms.
- The presence of these precursors again hints that this particular strain of Corynebacterium uterequi could potentially be pathogenic, and contributes more to our understanding of the virulence of this bacterium.
Cite This Article
APA
Rückert C, Kriete M, Jaenicke S, Winkler A, Tauch A.
(2015).
Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare.
Genome Announc, 3(4), e00783-15.
https://doi.org/10.1128/genomeA.00783-15 Publication
Researcher Affiliations
- Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Bioinformatics Resource Facility (BRF), Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
- Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany tauch@cebitec.uni-bielefeld.de.
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