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Home / Equine Research Bank / Lett Appl Microbiol / Virulence markers associated with Trueperella pyogenes infec...
Letters in applied microbiology2017; 65(2); 125-132; doi: 10.1111/lam.12757

Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals.

Abstract: Trueperella pyogenes is an opportunistic pathogen that causes diverse pyogenic infections in livestock. The genes that encode the exotoxin pyolysin (plo) and other putative factors that promote adhesion of pathogen to host cells (fimbriae fimA, fimC, fimE, fimG, neuraminidases nanH, nanP, and collagen-binding protein cbpA) have been associated with virulence, particularly in mastitis and uterus infections of dairy cows. However, the role of these virulence markers in the pathogenicity of the agent in domestic animals infections still is incompletely understood. The genes plo, fimA, fimC, fimE, fimG, nanH, nanP, and cbpA were investigated in 71 T. pyogenes strains recovered from cattle, sheep, goats, dogs, equines, and a pig, recovered from mastitis (n = 35), and non-mastitis (n = 36) cases (abscesses, reproductive tract diseases, pneumonia, lymphadenitis, encephalitis). The most common genes harboured by the isolates were: plo (71/71 = 100·0%), fimA (70/71 = 98·6%), nanP (56/71 = 78·9%), fimE (53/71 = 74·6%), fimC (46/71 = 64·8%) and nanH (45/71 = 63·4%), whereas cbpA (6/71 = 8·4%) and fimG (4/71 = 5·6%) were uncommon. The most frequent genotypes were plo/fimA/fimE/fimC/nanH/nanP (17/71 = 23·9%), plo/fimA/fimE/nanH/nanP (13/71 = 18·3%), and plo/fimA/fimE/fimC/nanP (11/71 = 15·5%). No association was observed between the presence of genes vs clinical signs or host species. To the best of our knowledge, this is the first report on aforementioned virulence factors of pathogen detected in diseased horses and dogs. Conclusions: The role of particular virulence factors of Trueperella pyogenes that determine different pyogenic infections among domestic animals is poorly understood. Eight putative virulence genes and genotype profiles of 71 isolates were investigated among different clinical manifestations in domestic animals. The most common genes were plo (71/71 = 100·0%), fimA (70/71 = 98·6%), nanP (56/71 = 78·9%), fimE (53/71 = 74·6%), fimC (46/71 = 64·8%) and nanH (45/71 = 63·4%), whereas plo/fimA/fimE/fimC/nanH/nanP (17/71 = 23·9%), plo/fimA/fimE/nanH/nanP (13/71 = 18·3%), and plo/fimA/fimE/fimC/nanP (11/71 = 15·5%) were the most frequent genotypes. Studies involving virulence factors are critical in the investigation of molecular epidemiology, pathogenicity, and hypothetical differences in the virulence among T. pyogenes strains from different geographical areas.
© 2017 The Society for Applied Microbiology.
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Publication Date: 2017-06-01 PubMed ID: 28561264DOI: 10.1111/lam.12757Google Scholar: Lookup
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Summary

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This study investigates the role of different virulence factors in Trueperella pyogenes, an opportunistic pathogen causing infection in various domestic animals, with a view to better understand its pathogenicity. The research analysed the presence of certain genes in 71 strains of the bacteria, originating from different animals and health conditions, to determine commonalities and potential correlations to clinical symptoms or specific host species.

Research Methodology

  • The researchers focused on Trueperella pyogenes, a pathogen known to cause numerous infections in animals. Previous research had indicated that certain genes within this organism – specifically, plo, fimA, fimC, fimE, fimG, nanH, nanP, and cbpA – might be fundamental to its virulence, particularly in mastitis and uterus infections in cows.
  • These eight genes were analyzed in 71 strains, derived from a variety of animals, including cattle, sheep, goats, dogs, equines, and pigs.
  • These strains were sourced from both mastitis and non-mastitis (abscesses, diseases in the reproductive tract, pneumonia, lymphadenitis, encephalitis) cases.

Research Findings

  • The results showed that the genes plo and fimA were the most common, found in 100% and 98.6% of strains, respectively.
  • Other prevalent genes included nanP (78.9%), fimE (74.6%), fimC (64.8%), and nanH (63.4%).
  • In contrast, cbpA and fimG were rare, present in only 8.4% and 5.6% of strains respectively.
  • No association was found between the presence of specific genes and clinical signs or the species of the host.
  • The virulence factors were detected in horses and dogs for the first time.

Conclusion and Significance

  • The study concludes that despite identifying the prevalence of certain genes, the precise role these virulence factors play in determining the variety of infections caused by Trueperella pyogenes in domestic animals remains unclear.
  • The identification and analysis of these eight putative virulence genes in 71 isolates across different clinical manifestations constitutes a significant contribution to the body of academic research and evidence on the subject.
  • The most common gene profiles were plo/fimA/fimE/fimC/nanH/nanP (23.9%), plo/fimA/fimE/nanH/nanP (18.3%), and plo/fimA/fimE/fimC/nanP (15.5%).
  • Ultimately, these studies are crucial for understanding the molecular epidemiology, pathogenicity, and hypothetical differences in virulence among Trueperella pyogenes strains from different geographical areas. This knowledge could potentially inform more effective diagnostics, treatments, and prevention strategies for infections caused by this pathogen.

Cite This Article

APA
Risseti RM, Zastempowska E, Twarużek M, Lassa H, Pantoja JCF, de Vargas APC, Guerra ST, Bolaños CAD, de Paula CL, Alves AC, Colhado BS, Portilho FVR, Tasca C, Lara GHB, Ribeiro MG. (2017). Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals. Lett Appl Microbiol, 65(2), 125-132. https://doi.org/10.1111/lam.12757

Publication

Letters in applied microbiology
ISSN: 1472-765X
NlmUniqueID: 8510094
Country: England
Language: English
Volume: 65
Issue: 2
Pages: 125-132

Researcher Affiliations

Risseti, R M
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Zastempowska, E
  • Department of Physiology and Toxicology, Institute of Experimental Biology, Faculty of Natural Sciences, Kazimierz Wielki University, Bydgoszcz, Poland.
Twarużek, M
  • Department of Physiology and Toxicology, Institute of Experimental Biology, Faculty of Natural Sciences, Kazimierz Wielki University, Bydgoszcz, Poland.
Lassa, H
  • Milk Testing Laboratory, Bydgoszcz, Poland.
Pantoja, J C F
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
de Vargas, A P C
  • Department of Preventive Veterinary Medicine, Federal University of Santa Maria-UFSM, Santa Maria, Brazil.
Guerra, S T
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Bolaños, C A D
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
de Paula, C L
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Alves, A C
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Colhado, B S
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Portilho, F V R
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Tasca, C
  • Department of Preventive Veterinary Medicine, Federal University of Santa Maria-UFSM, Santa Maria, Brazil.
Lara, G H B
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.
Ribeiro, M G
  • Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP - São Paulo State University, Botucatu, Brazil.

MeSH Terms

  • Actinomycetales Infections / microbiology
  • Actinomycetales Infections / veterinary
  • Animals
  • Arcanobacterium / genetics
  • Arcanobacterium / pathogenicity
  • Bacterial Proteins / genetics
  • Bacterial Toxins / genetics
  • Cattle
  • Dogs
  • Female
  • Genotype
  • Goats
  • Hemolysin Proteins / genetics
  • Horses
  • Livestock
  • Mastitis / microbiology
  • Mastitis / veterinary
  • Pets
  • Sheep
  • Swine
  • Virulence
  • Virulence Factors / genetics

Citations

This article has been cited 25 times.
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