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Home / Equine Research Bank / J Exp Orthop / What quantitative mechanical loading stimulates in vitro cul...
Journal of experimental orthopaedics2015; 2(1); 15; doi: 10.1186/s40634-015-0029-x

What quantitative mechanical loading stimulates in vitro cultivation best?

Abstract: Articular cartilage has limited regeneration capacities. One of the factors that appear to affect the in vitro cultivation of articular cartilage is mechanical stimulation. So far, no combination of parameters has been identified that offers the best results. The goal is to review the literature in search of the best available set of quantitative mechanical stimuli that lead to optimal in vitro cultivation.The databases Scopus and PubMed were used to survey the literature, and strict in- and exclusion criteria were applied regarding the presence of quantitative data. The review was performed by studying the type of loading (hydrostatic compression or direct compression), the loading magnitude, the frequency and the loading regime (duration of the loading) in comparison to quantitative evidence of cartilage quality response (cellular, signaling and mechanical).Thirty-three studies met all criteria of which 8 studied human, 20 bovine, 2 equine, 1 ovine, 1 porcine and 1 canine cells using four different types of cultivated constructs. Six studies investigated loading magnitude within the same setup, three studies the frequency, and seven the loading regime. Nine studies presented mechanical tissue response. The studies suggest that a certain threshold exits for enhanced cartilage in vitro cultivation of explants (>20 % strain and 0.5 Hz), and that chondrocyte-seeded cultivated constructs show best results when loaded with physiological mechanical stimuli. That is a loading pressure between 5-10 MPa and a loading frequency of 1 Hz exerted at intermittent intervals for a period of a week or longer. Critical aspects remain to be answered for translation into in vivo therapies.
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Publication Date: 2015-06-19 PubMed ID: 26914883PubMed Central: PMC4538712DOI: 10.1186/s40634-015-0029-xGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is reviewing existing literature to try and identify the best set of mechanical stimuli which can enhance in vitro cultivation of articular cartilage.

Study Objective and Method

  • The primary objective of this research was to identify the optimum quantitative mechanical stimuli that improve in vitro cultivation of articular cartilage. This is a significant study because articular cartilage struggles with regenerative abilities.
  • The method employed involved a comprehensive review of available literature. The researchers used databases such as Scopus and PubMed, applying strict criteria on their selection process, particularly insisting on the availability of quantitative data.
  • The research focused on analyzing the type of loading – whether it was hydrostatic compression or direct compression, the loading magnitude, the frequency of loading, and the duration of the loading.
  • Additionally, the research also studied the cartilage quality response, including changes at the cellular level, signaling, and mechanical alterations.

Key Findings

  • Out of numerous studies, 33 met the criteria, and the research used animal cells varying from humans, bovines, equines, ovine, porcine, and canine in different types of cultured constructs. The results indicated a higher efficacy of in vitro cultivation of explants if the strain exceeded 20% at a frequency of 0.5 Hz.
  • For chondrocyte-seeded cultivated constructs, the best results were realized with physiological mechanical stimuli involving loading pressure within 5-10 MPa and a frequency of 1 Hz. These conditions were applied intermittently over a period of a week or more. This data progresses our understanding of practical in vitro cultivation strategies for articular cartilage.
  • Despite the noteworthy findings, the study underscores the need for further research to address critical aspects for successful in vivo therapies. This forms the basis for future studies and potential direction for exploratory experiments.

Cite This Article

APA
Natenstedt J, Kok AC, Dankelman J, Tuijthof GJ. (2015). What quantitative mechanical loading stimulates in vitro cultivation best? J Exp Orthop, 2(1), 15. https://doi.org/10.1186/s40634-015-0029-x

Publication

Journal of experimental orthopaedics
ISSN: 2197-1153
NlmUniqueID: 101653750
Country: Germany
Language: English
Volume: 2
Issue: 1
Pages: 15
PII: 15

Researcher Affiliations

Natenstedt, Jerry
  • Department of Biomechanical Engineering, Faculty of Mechanical, Materials and Maritime Engineering, Delft University of Technology, Mekelweg 2, Delft, 2628 CD, The Netherlands. J.Natenstedt@student.tudelft.nl.
Kok, Aimee C
  • Department of Orthopedic Surgery, Academic Medical Centre, Meibergdreef 9, Amsterdam, AZ, 1105, The Netherlands. a.kok@amc.uva.nl.
Dankelman, Jenny
  • Department of Biomechanical Engineering, Faculty of Mechanical, Materials and Maritime Engineering, Delft University of Technology, Mekelweg 2, Delft, 2628 CD, The Netherlands. j.dankelman@tudelft.nl.
Tuijthof, Gabrielle Jm
  • Department of Biomechanical Engineering, Faculty of Mechanical, Materials and Maritime Engineering, Delft University of Technology, Mekelweg 2, Delft, 2628 CD, The Netherlands. g.j.m.tuijthof@tudelft.nl.
  • Department of Orthopedic Surgery, Academic Medical Centre, Meibergdreef 9, Amsterdam, AZ, 1105, The Netherlands. g.j.m.tuijthof@tudelft.nl.

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Citations

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