Whole-genome scan identifies quantitative trait loci for chronic pastern dermatitis in German draft horses.
Abstract: Chronic pastern dermatitis (CPD), also known as chronic progressive lymphedema (CPL), is a skin disease that affects draft horses. This disease causes painful lower-leg swelling, nodule formation, and skin ulceration, interfering with movement. The aim of this whole-genome scan was to identify quantitative trait loci (QTL) for CPD in German draft horses. We recorded clinical data for CPD in 917 German draft horses and collected blood samples from these horses. Of these 917 horses, 31 paternal half-sib families comprising 378 horses from the breeds Rhenish German, Schleswig, Saxon-Thuringian, and South German were chosen for genotyping. Each half-sib family was constituted by only one draft horse breed. Genotyping was done for 318 polymorphic microsatellites evenly distributed on all equine autosomes and the X chromosome with a mean distance of 7.5 Mb. An across-breed multipoint linkage analysis revealed chromosome-wide significant QTL on horse chromosomes (ECA) 1, 9, 16, and 17. Analyses by breed confirmed the QTL on ECA1 in South German and the QTL on ECA9, 16, and 17 in Saxon-Thuringian draft horses. For the Rhenish German and Schleswig draft horses, additional QTL on ECA4 and 10 and for the South German draft horses an additional QTL on ECA7 were found. This is the first whole-genome scan for CPD in draft horses and it is an important step toward the identification of candidate genes.
Publication Date: 2009-12-29 PubMed ID: 20039044DOI: 10.1007/s00335-009-9244-zGoogle Scholar: Lookup
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- Journal Article
Summary
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This research studied a skin disease in German draft horses, named chronic pastern dermatitis (CPD), and identified specific chromosomes in the horse genome that influence the occurrence of the disease. Through sampling and genotyping various draft horse breeds, the researchers found significant quantitative trait loci (QTL) on four specific chromosomes.
Chronic Pastern Dermatitis (CPD) and Objective of the Study
- This research investigates Chronic Pastern Dermatitis (CPD), a harmful skin disease that causes painful swelling, skin ulceration, and nodule formation in the lower legs of draft horses, affecting their ability to move.
- The purpose of the whole-genome scan was to discover if certain locations in the genome (quantitative trait loci or QTL) are associated with CPD in German draft horses.
Methodology
- For the research, clinical data of CPD were recorded from 917 German draft horses and blood samples were collected from them.
- From the total sample size, 31 paternal half-sib families—an aggregation of siblings that share the same father—were chosen. These families comprised of 378 horses hailing from four different German draft horse breeds.
- The genotyping was done on 318 polymorphic microsatellites—bits of DNA that are assessed for genetic differences—across the equine autosomes (non-sex chromosomes) and the X chromosome.
Findings
- Through multipoint linkage analysis—a statistical method used to find out which genes are inherited together—the researchers found significant QTL for CPD on four horse chromosomes (ECA 1, 9, 16, and 17).
- The QTL on ECA1 was confirmed in South German draft horses, and those on ECA9, 16, and 17 in Saxon-Thuringian draft horses.
- Rhenish German and Schleswig draft horses had additional QTL on ECA4 and 10, and for the South German draft horses, an additional QTL on ECA7 was located.
Importance and Implications of the Research
- This study is the first whole-genome scan for CPD in draft horses, hence it paves the way for future research. The identification of these QTLs is crucial as it can lead to the discovery of candidate genes that cause CPD.
- Understanding the genetic factors contributing to CPD can help in developing prevention strategies and treatments for this disease, improving the well-being and quality of life of draft horses.
Cite This Article
APA
Mittmann EH, Mömke S, Distl O.
(2009).
Whole-genome scan identifies quantitative trait loci for chronic pastern dermatitis in German draft horses.
Mamm Genome, 21(1-2), 95-103.
https://doi.org/10.1007/s00335-009-9244-z Publication
Researcher Affiliations
- Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559, Hannover, Germany.
MeSH Terms
- Animals
- Chromosomes, Mammalian / genetics
- Chronic Disease
- Dermatitis / genetics
- Dermatitis / veterinary
- Foot Diseases / genetics
- Foot Diseases / veterinary
- Genome-Wide Association Study
- Genotype
- Horse Diseases / genetics
- Horses / genetics
- Lod Score
- Lymphedema / genetics
- Lymphedema / veterinary
- Microsatellite Repeats
- Quantitative Trait Loci
References
This article includes 22 references
- J Cell Biochem. 2009 Apr 15;106(6):1123-35
- Mol Cell Biol. 2006 Aug;26(15):5595-602
- Biometrics. 1994 Mar;50(1):118-27
- Am J Hum Genet. 1997 Nov;61(5):1179-88
- Vet J. 2007 Sep;174(2):397-9
- Lymphat Res Biol. 2003;1(3):191-9
- Anim Genet. 2004 Aug;35(4):270-7
- Genome Res. 2003 Apr;13(4):742-51
- Am J Hum Genet. 1996 Jun;58(6):1347-63
- Vet Dermatol. 2005 Dec;16(6):373-84
- Anim Genet. 2010 Apr;41(2):222
- Lymphat Res Biol. 2006;4(2):67-72
- FASEB J. 2006 Jul;20(9):1525-7
- Bioinformatics. 2005 Aug 15;21(16):3445-7
- Nat Genet. 2002 Jan;30(1):97-101
- Cytogenet Genome Res. 2008;122(1):28-36
- Berl Munch Tierarztl Wochenschr. 2004 Jan-Feb;117(1-2):72-5
- Cytogenet Genome Res. 2007;116(4):256-62
- Genomics. 2006 Jan;87(1):1-29
- Berl Munch Tierarztl Wochenschr. 2004 Mar-Apr;117(3-4):148-52
- J Hered. 2007 May-Jun;98(3):267-71
- Cytogenet Genome Res. 2005;111(1):5-15
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