Whole-Genome Sequences of an Abortive Bacillus safensis Strain Isolated from a Mare’s Uterus.
Abstract: This is a report of two Bacillus safensis genomes sequenced from separate cultures isolated from the uterus of a 16-year-old Westphalian mare that aborted a dead fetus. This strain represents the first case of a B. safensis-associated equine abortion and the first case of infection caused by this bacterium.
Copyright © 2020 Little et al.
Publication Date: 2020-05-14 PubMed ID: 32409543PubMed Central: PMC7225542DOI: 10.1128/MRA.00342-20Google Scholar: Lookup
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Summary
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The research report describes the whole genome sequencing of a pathogenic strain of Bacillus safensis, isolated from a mare’s uterus which led to abortion. This is the first reported case where B. safensis is associated with horse abortion, as well as being the first infection case of this bacterium.
Introduction
- The core objective of this study was to sequence the genome of the Bacillus safensis bacterium that was isolated from the uterus of an equine (a 16-year-old Westphalian mare) that had aborted a dead foetus.
- This particular strain of Bacillus safensis is highly significant as it represents the inaugural documented case in which this bacterium has been associated with equine abortion.
- Prior to this, there were no reported cases of infection caused by this bacterium, hence this research marks a major discovery in microbial genomics pertaining to equine diseases.
Methodology
- Two separate cultures from the uterus of the mare were obtained for the study.
- The genomes of Bacillus safensis from each of these cultures were sequenced independently.
- The resulting genomic data was then matched and compared to look for any significant variations and potential implications.
Results and Findings
- The whole-genome sequencing of the abortive Bacillus safensis strain showed that it was similar to the bacterium’s known sequences but variations were detected.
- These variations in the genome could potentially be the reason behind the previously unknown pathogenicity of the bacterium, leading to equine abortion.
Conclusion
- The findings provide a novel insight into the Bacillus safensis strain and its associated pathogenicity.
- This study could open up new avenues for further research into the strain, the diseases it could cause, possibilities for treatment or prevention, and overall equine health.
Shedding light on this overlooked bacterium, this study represents a significant leap in our comprehension of equine microbial diseases. Improvement in whole-genome sequencing technologies and continuous research might lead to thorough exploration of the relationships between microorganisms and host health.
Cite This Article
APA
Little SV, Hillhouse AE, Lawhon SD.
(2020).
Whole-Genome Sequences of an Abortive Bacillus safensis Strain Isolated from a Mare’s Uterus.
Microbiol Resour Announc, 9(20).
https://doi.org/10.1128/MRA.00342-20 Publication
Researcher Affiliations
- Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Texas A&M Institute for Genome Sciences and Society, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA slawhon@cvm.tamu.edu.
Grant Funding
- U18 FD006171 / FDA HHS
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This article includes 9 references
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Citations
This article has been cited 2 times.- Chang YW, Sun YL, Chu E, Hung YY, Liao WC, Tsai SM, Lu TH, Huang PC, Yu CH, Lee SY, Chang HH, Lin DP. Streptococcus thermophilus iHA318 Improves Dry Eye Symptoms by Mitigating Ocular Surface Damage in a Mouse Model. Microorganisms 2024 Jun 27;12(7).
- Obata F, Murota H, Shibata S, Ozuru R, Fujii J. Investigation of Bacteria from Spoiled Bottled Salad Dressing Leading to Gas Explosion. Yonago Acta Med 2022 Aug;65(3):207-214.
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