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Home / Equine Research Bank / Chromosome Res / Zoo-FISH delineates conserved chromosomal segments in horse ...
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1996; 4(3); 218-225; doi: 10.1007/BF02254963

Zoo-FISH delineates conserved chromosomal segments in horse and man.

Abstract: Human chromosome specific libraries (CSLs) were individually applied to equine metaphase chromosomes using the fluorescence in situ hybridization (FISH) technique. All CSLs, except Y, showed painting signals on one or several horse chromosomes. In total 43 conserved chromosomal segments were painted. Homoeology could not, however, be detected for some segments of the equine genome. This is most likely related to the very weak signals displayed by some libraries, rather than to the absence of similarity with the human genome. In spite of divergence from the human genome, dated 70-80 million years ago, a fairly high degree of synteny conservation was observed. In seven cases, whole chromosome synteny was detected between the two species. The comparative painting results agreed completely with the limited gene mapping data available in horses, and also enabled us provisionally to assign one linkage group (U2) and one syntenic group (NP, MPI, IDH2) to specific equine chromosomes. Chromosomal assignments of three other syntenic groups are also proposed. The findings of this study will be of significant use in the expansion of the hitherto poorly developed equine gene map.
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Publication Date: 1996-04-01 PubMed ID: 8793207DOI: 10.1007/BF02254963Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the application of human chromosome specific libraries to equine (horse) metaphase chromosomes to assess their similarities and conservation. Despite a divergence that occurred 70-80 million years ago, a fairly high degree of synteny conservation was identified the two species, with some whole chromosome synteny detected.

Introduction and Methodology

  • The study utilizes fluorescence in situ hybridization (FISH), a powerful technique that allows for visual identification of specific DNA sequences on chromosomes.
  • Through FISH, human chromosome specific libraries (collections of DNA clones from one specific human chromosome) were applied individually to horse metaphase chromosomes. Metaphase is a stage in cell division where chromosomes are at their most condensed and coiled stage, making them easy to view under a microscope.
  • All human chromosome libraries, excluding Y chromosome, displayed signals on one or multiple horse chromosomes, revealing shared segments between human and horse chromosomes.

Results and Analysis

  • The study identified 43 conserved chromosomal segments shared between horses and humans. Conserved segments are the preserved sections of DNA that have remained unchanged through evolutionary time due to their critical role in survival or reproduction.
  • Some segments of the equine genome couldn’t display homoeology (equivalent chromosomal segments) with humans. This was attributed to the weak signals emitted by certain libraries, not necessarily because there was no similarity with the human genome.
  • Despite the evolutionary divergence between horses and humans estimated to be 70-80 million years ago, the research demonstrated a considerable degree of synteny conservation. Synteny refers to the presence of two or more genes on the same chromosome, implying an evolutionary relationship.
  • Whole chromosome synteny was identified in seven instances between horses and humans, further substantiating the similarities at the chromosomal level between these two species.

Findings and Conclusion

  • The research findings not only corroborated the limited gene mapping data available for horses but it also allowed the assignment of one linkage group (U2) and one syntenic group (NP, MPI, IDH2) to specific horse chromosomes.
  • The study also made chromosomal assignments for three other syntenic groups.
  • The results of this study are highly significant and useful for expanding the equine gene map, which has been relatively underdeveloped so far.

Cite This Article

APA
Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP. (1996). Zoo-FISH delineates conserved chromosomal segments in horse and man. Chromosome Res, 4(3), 218-225. https://doi.org/10.1007/BF02254963

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 4
Issue: 3
Pages: 218-225

Researcher Affiliations

Raudsepp, T
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Frönicke, L
    Scherthan, H
      Gustavsson, I
        Chowdhary, B P

          MeSH Terms

          • Animals
          • Chromosome Mapping / veterinary
          • Chromosomes / ultrastructure
          • Chromosomes, Human / ultrastructure
          • Evolution, Molecular
          • Hominidae / genetics
          • Horses / genetics
          • Humans
          • In Situ Hybridization, Fluorescence
          • Male
          • Phylogeny
          • Species Specificity

          References

          This article includes 22 references
          1. Stanyon R, Arnold N, Koehler U, Bigoni F, Wienberg J. Chromosomal painting shows that "marked chromosomes" in lesser apes and Old World monkeys are not homologous and evolved by convergence.. Cytogenet Cell Genet 1995;68(1-2):74-8.
            pubmed: 7956365doi: 10.1159/000133894google scholar: lookup
          2. Bailey E, Graves KT, Cothran EG, Reid R, Lear TL, Ennis RB. Synteny-mapping horse microsatellite markers using a heterohybridoma panel.. Anim Genet 1995 Jun;26(3):177-80.
            pubmed: 7793685doi: 10.1111/j.1365-2052.1995.tb03158.xgoogle scholar: lookup
          3. Andersson L, Haley CS, Ellegren H, Knott SA, Johansson M, Andersson K, Andersson-Eklund L, Edfors-Lilja I, Fredholm M, Hansson I. Genetic mapping of quantitative trait loci for growth and fatness in pigs.. Science 1994 Mar 25;263(5154):1771-4.
            pubmed: 8134840doi: 10.1126/science.8134840google scholar: lookup
          4. Hayes H. Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes.. Cytogenet Cell Genet 1995;71(2):168-74.
            pubmed: 7656590doi: 10.1159/000134100google scholar: lookup
          5. Collins C, Kuo WL, Segraves R, Fuscoe J, Pinkel D, Gray JW. Construction and characterization of plasmid libraries enriched in sequences from single human chromosomes.. Genomics 1991 Dec;11(4):997-1006.
            pubmed: 1783406doi: 10.1016/0888-7543(91)90025-agoogle scholar: lookup
          6. Vooijs M, Yu LC, Tkachuk D, Pinkel D, Johnson D, Gray JW. Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR.. Am J Hum Genet 1993 Mar;52(3):586-97.
            pubmed: 8447324
          7. Edwards JH. Comparative genome mapping in mammals.. Curr Opin Genet Dev 1994 Dec;4(6):861-7.
            pubmed: 7888756doi: 10.1016/0959-437x(94)90071-xgoogle scholar: lookup
          8. Lundin LG. Evolution of the vertebrate genome as reflected in paralogous chromosomal regions in man and the house mouse.. Genomics 1993 Apr;16(1):1-19.
            pubmed: 8486346doi: 10.1006/geno.1993.1133google scholar: lookup
          9. Rønne M. Putative fragile sites in the horse karyotype.. Hereditas 1992;117(2):127-36.
            pubmed: 1459856doi: 10.1111/j.1601-5223.1992.tb00166.xgoogle scholar: lookup
          10. Rettenberger G, Klett C, Zechner U, Bruch J, Just W, Vogel W, Hameister H. ZOO-FISH analysis: cat and human karyotypes closely resemble the putative ancestral mammalian karyotype.. Chromosome Res 1995 Dec;3(8):479-86.
            pubmed: 8581300doi: 10.1007/BF00713962google scholar: lookup
          11. O'Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG. Anchored reference loci for comparative genome mapping in mammals.. Nat Genet 1993 Feb;3(2):103-12.
            pubmed: 8499943doi: 10.1038/ng0293-103google scholar: lookup
          12. Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H. Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting.. Genomics 1995 Mar 20;26(2):372-8.
            pubmed: 7601464doi: 10.1016/0888-7543(95)80222-8google scholar: lookup
          13. Bradley RD, Wichman HA. Rapidly evolving repetitive DNAs in a conservative genome: a test of factors that affect chromosomal evolution.. Chromosome Res 1994 Sep;2(5):354-60.
            pubmed: 7981939doi: 10.1007/BF01552794google scholar: lookup
          14. Richer CL, Power MM, Klunder LR, McFeely RA, Kent MG. Standard karyotype of the domestic horse (Equus caballus). Committee for standardized karyotype of Equus caballus. The Second International Conference for Standardization of Domestic Animal Karyotypes, INRA, Jouy-en Josas, France, 22nd-26th May 1989.. Hereditas 1990;112(3):289-93.
            pubmed: 1976611doi: 10.1111/j.1601-5223.1990.tb00069.xgoogle scholar: lookup
          15. Oakenfull EA, Buckle VJ, Clegg JB. Localization of the horse (Equus caballus) alpha-globin gene complex to chromosome 13 by fluorescence in situ hybridization.. Cytogenet Cell Genet 1993;62(2-3):136-8.
            pubmed: 8428512doi: 10.1159/000133456google scholar: lookup
          16. Scherthan H, Cremer T, Arnason U, Weier HU, Lima-de-Faria A, Frönicke L. Comparative chromosome painting discloses homologous segments in distantly related mammals.. Nat Genet 1994 Apr;6(4):342-7.
            pubmed: 8054973doi: 10.1038/ng0494-342google scholar: lookup
          17. Wienberg J, Jauch A, Stanyon R, Cremer T. Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization.. Genomics 1990 Oct;8(2):347-50.
            pubmed: 2249853doi: 10.1016/0888-7543(90)90292-3google scholar: lookup
          18. Williams H, Richards CM, Konfortov BA, Miller JR, Tucker EM. Synteny mapping in the horse using horse-mouse heterohybridomas.. Anim Genet 1993 Aug;24(4):257-60.
            pubmed: 8239069doi: 10.1111/j.1365-2052.1993.tb00308.xgoogle scholar: lookup
          19. MacLennan DH, Duff C, Zorzato F, Fujii J, Phillips M, Korneluk RG, Frodis W, Britt BA, Worton RG. Ryanodine receptor gene is a candidate for predisposition to malignant hyperthermia.. Nature 1990 Feb 8;343(6258):559-61.
            pubmed: 1967823doi: 10.1038/343559a0google scholar: lookup
          20. Solinas-Toldo S, Lengauer C, Fries R. Comparative genome map of human and cattle.. Genomics 1995 Jun 10;27(3):489-96.
            pubmed: 7558031doi: 10.1006/geno.1995.1081google scholar: lookup
          21. Georges M, Dietz AB, Mishra A, Nielsen D, Sargeant LS, Sorensen A, Steele MR, Zhao X, Leipold H, Womack JE. Microsatellite mapping of the gene causing weaver disease in cattle will allow the study of an associated quantitative trait locus.. Proc Natl Acad Sci U S A 1993 Feb 1;90(3):1058-62.
            pubmed: 8430074doi: 10.1073/pnas.90.3.1058google scholar: lookup
          22. OHNO S, BECAK W, BECAK ML. X-AUTOSOME RATIO AND THE BEHAVIOR PATTERN OF INDIVIDUAL X-CHROMOSOMES IN PLACENTAL MAMMALS.. Chromosoma 1964 Apr 1;15:14-30.
            pubmed: 14171167doi: 10.1007/BF00326912google scholar: lookup

          Citations

          This article has been cited 39 times.
          1. Brosnahan MM, Brooks SA, Antczak DF. Equine clinical genomics: A clinician's primer.. Equine Vet J 2010 Oct;42(7):658-70.
            doi: 10.1111/j.2042-3306.2010.00166.xpubmed: 20840582google scholar: lookup
          2. Musilova P, Kubickova S, Horin P, Vodicka R, Rubes J. Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes.. Chromosome Res 2009;17(6):783-90.
            doi: 10.1007/s10577-009-9069-3pubmed: 19731053google scholar: lookup
          3. Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome.. Cytogenet Genome Res 2008;122(1):28-36.
            doi: 10.1159/000151313pubmed: 18931483google scholar: lookup
          4. McNamara G, Difilippantonio MJ, Ried T. Microscopy and image analysis.. Curr Protoc Hum Genet 2005 Aug;Chapter 4:Unit 4.4.
            doi: 10.1002/0471142905.hg0404s46pubmed: 18428379google scholar: lookup
          5. Trifonov VA, Stanyon R, Nesterenko AI, Fu B, Perelman PL, O'Brien PC, Stone G, Rubtsova NV, Houck ML, Robinson TJ, Ferguson-Smith MA, Dobigny G, Graphodatsky AS, Yang F. Multidirectional cross-species painting illuminates the history of karyotypic evolution in Perissodactyla.. Chromosome Res 2008;16(1):89-107.
            doi: 10.1007/s10577-007-1201-7pubmed: 18293107google scholar: lookup
          6. Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence.. Chromosome Res 2008;16(1):109-27.
            doi: 10.1007/s10577-008-1204-zpubmed: 18274866google scholar: lookup
          7. Rios JJ, Perelygin AA, Long MT, Lear TL, Zharkikh AA, Brinton MA, Adelson DL. Characterization of the equine 2'-5' oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes.. BMC Genomics 2007 Sep 7;8:313.
            doi: 10.1186/1471-2164-8-313pubmed: 17822564google scholar: lookup
          8. Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals.. Mamm Genome 2005 Aug;16(8):631-49.
            doi: 10.1007/s00335-005-0023-1pubmed: 16180145google scholar: lookup
          9. Bernot A, Weissenbach J. Estimation of the extent of synteny between Tetraodon nigroviridis and Homo sapiens genomes.. J Mol Evol 2004 Oct;59(4):556-69.
            doi: 10.1007/s00239-004-2649-0pubmed: 15638467google scholar: lookup
          10. Paulis M, Moralli D, Bensi M, De Carli L, Raimondi E. Isolation from the horse genome of a new DNA transposon belonging to the Tigger family.. Mamm Genome 2004 May;15(5):399-403.
            doi: 10.1007/s00335-004-3040-6pubmed: 15170229google scholar: lookup
          11. Yang F, Fu B, O'Brien PC, Nie W, Ryder OA, Ferguson-Smith MA. Refined genome-wide comparative map of the domestic horse, donkey and human based on cross-species chromosome painting: insight into the occasional fertility of mules.. Chromosome Res 2004;12(1):65-76.
            doi: 10.1023/b:chro.0000009298.02689.8apubmed: 14984103google scholar: lookup
          12. Richard F, Lombard M, Dutrillaux B. Reconstruction of the ancestral karyotype of eutherian mammals.. Chromosome Res 2003;11(6):605-18.
            doi: 10.1023/a:1024957002755pubmed: 14516069google scholar: lookup
          13. Frönicke L, Wienberg J, Stone G, Adams L, Stanyon R. Towards the delineation of the ancestral eutherian genome organization: comparative genome maps of human and the African elephant (Loxodonta africana) generated by chromosome painting.. Proc Biol Sci 2003 Jul 7;270(1522):1331-40.
            doi: 10.1098/rspb.2003.2383pubmed: 12965023google scholar: lookup
          14. Lundin LG, Larhammar D, Hallböök F. Numerous groups of chromosomal regional paralogies strongly indicate two genome doublings at the root of the vertebrates.. J Struct Funct Genomics 2003;3(1-4):53-63.
            pubmed: 12836685
          15. Chowdhary BP, Raudsepp T, Kata SR, Goh G, Millon LV, Allan V, Piumi F, Guérin G, Swinburne J, Binns M, Lear TL, Mickelson J, Murray J, Antczak DF, Womack JE, Skow LC. The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes.. Genome Res 2003 Apr;13(4):742-51.
            doi: 10.1101/gr.917503pubmed: 12671008google scholar: lookup
          16. Santani A, Raudsepp T, Chowdhary BP. Interstitial telomeric sites and NORs in Hartmann's zebra (Equus zebra hartmannae) chromosomes.. Chromosome Res 2002;10(7):527-34.
            doi: 10.1023/a:1020945400949pubmed: 12498342google scholar: lookup
          17. Volleth M, Heller KG, Pfeiffer RA, Hameister H. A comparative ZOO-FISH analysis in bats elucidates the phylogenetic relationships between Megachiroptera and five microchiropteran families.. Chromosome Res 2002;10(6):477-97.
            doi: 10.1023/a:1020992330679pubmed: 12489830google scholar: lookup
          18. Raudsepp T, Chowdhary BP. Correspondence of human chromosomes 9, 12, 15, 16, 19 and 20 with donkey chromosomes refines homology between horse and donkey karyotypes.. Chromosome Res 2001;9(8):623-9.
            doi: 10.1023/a:1012948122600pubmed: 11778685google scholar: lookup
          19. Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis.. Chromosome Res 2001;9(1):53-9.
            doi: 10.1023/a:1026743700819pubmed: 11272792google scholar: lookup
          20. Raudsepp T, Christensen K, Chowdhar BP. Cytogenetics of donkey chromosomes: nomenclature proposal based on GTG-banded chromosomes and depiction of NORs and telomeric sites.. Chromosome Res 2000;8(8):659-70.
            doi: 10.1023/a:1026707002538pubmed: 11196129google scholar: lookup
          21. Richard F, Lombard M, Dutrillaux B. Phylogenetic origin of human chromosomes 7, 16, and 19 and their homologs in placental mammals.. Genome Res 2000 May;10(5):644-51.
            doi: 10.1101/gr.10.5.644pubmed: 10810086google scholar: lookup
          22. Shiue Y-L, Millon LV, Skow LC, Honeycutt D, Murray JD, Bowling AT. Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes.. Chromosome Res 2000;8(1):45-55.
            doi: 10.1023/a:1009275102977pubmed: 10730588google scholar: lookup
          23. Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. A comparative gene map of the horse (Equus caballus).. Genome Res 1999 Dec;9(12):1239-49.
            doi: 10.1101/gr.9.12.1239pubmed: 10613847google scholar: lookup
          24. Saccone S, Federico C, Solovei I, Croquette MF, Della Valle G, Bernardi G. Identification of the gene-richest bands in human prometaphase chromosomes.. Chromosome Res 1999;7(5):379-86.
            doi: 10.1023/a:1009220131225pubmed: 10515213google scholar: lookup
          25. Shetty S, Griffin DK, Graves JA. Comparative painting reveals strong chromosome homology over 80 million years of bird evolution.. Chromosome Res 1999;7(4):289-95.
            doi: 10.1023/a:1009278914829pubmed: 10461874google scholar: lookup
          26. Raudsepp T, Chowdhary BP. Construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey.. Chromosome Res 1999;7(2):103-14.
            doi: 10.1023/a:1009234814635pubmed: 10328622google scholar: lookup
          27. Volleth M, Klett C, Kollak A, Dixkens C, Winter Y, Just W, Vogel W, Hameister H. ZOO-FISH analysis in a species of the order Chiroptera: Glossophaga soricina (Phyllostomidae).. Chromosome Res 1999;7(1):57-64.
            doi: 10.1023/a:1009227428727pubmed: 10219733google scholar: lookup
          28. Toder R, Xia Y, Bausch E. Interspecies comparative genome hybridization and interspecies representational difference analysis reveal gross DNA differences between humans and great apes.. Chromosome Res 1998 Sep;6(6):487-94.
            doi: 10.1023/a:1009208730186pubmed: 9865788google scholar: lookup
          29. Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome.. Genome Res 1998 Sep;8(9):951-66.
            doi: 10.1101/gr.8.9.951pubmed: 9750194google scholar: lookup
          30. Lear TL, Breen M, Ponce de Leon FA, Coogle L, Ferguson EM, Chambers TM, Bailey E. Cloning and chromosomal localization of MX1 and ETS2 to chromosome 26 of the horse (Equus caballus).. Chromosome Res 1998 Jun;6(4):333-5.
            doi: 10.1023/a:1009283126868pubmed: 9688525google scholar: lookup
          31. Morescalchi MA, Schempp W, Consigliere S, Bigoni F, Wienberg J, Stanyon R. Mapping chromosomal homology between humans and the black-handed spider monkey by fluorescence in situ hybridization.. Chromosome Res 1997 Dec;5(8):527-36.
            doi: 10.1023/a:1018489602312pubmed: 9451952google scholar: lookup
          32. Chaudhary R, Raudsepp T, Guan XY, Zhang H, Chowdhary BP. Zoo-FISH with microdissected arm specific paints for HSA2, 5, 6, 16, and 19 refines known homology with pig and horse chromosomes.. Mamm Genome 1998 Jan;9(1):44-9.
            doi: 10.1007/s003359900677pubmed: 9434944google scholar: lookup
          33. Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Committee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996.. Chromosome Res 1997 Nov;5(7):433-43.
            doi: 10.1023/a:1018408811881pubmed: 9421259google scholar: lookup
          34. Bowling AT, Millon LV, Dileanis S. Physical mapping of genetic markers to chromosome 30 using a trisomic horse and evidence for maternal origin of the extra chromosome.. Chromosome Res 1997 Sep;5(6):429-31.
            doi: 10.1023/a:1018456727811pubmed: 9364945google scholar: lookup
          35. Raudsepp T, Otte K, Rozell B, Chowdhary BP. FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11.. Mamm Genome 1997 Aug;8(8):569-72.
            doi: 10.1007/s003359900505pubmed: 9250862google scholar: lookup
          36. Frönicke L, Scherthan H. Zoo-fluorescence in situ hybridization analysis of human and Indian muntjac karyotypes (Muntiacus muntjak vaginalis) reveals satellite DNA clusters at the margins of conserved syntenic segments.. Chromosome Res 1997 Jun;5(4):254-61.
            doi: 10.1023/B:CHRO.0000032298.22346.46pubmed: 9244453google scholar: lookup
          37. Sjöberg A, Peelman LJ, Chowdhary BP. Application of three different methods to analyse fibre-FISH results obtained using four lambda clones from the porcine MHC III region.. Chromosome Res 1997 Jun;5(4):247-53.
            doi: 10.1023/a:1018419619634pubmed: 9244452google scholar: lookup
          38. Toder R, O'Neill RJ, Wienberg J, O'Brien PC, Voullaire L, Marshall-Graves JA. Comparative chromosome painting between two marsupials: origins of an XX/XY1Y2 sex chromosome system.. Mamm Genome 1997 Jun;8(6):418-22.
            doi: 10.1007/s003359900459pubmed: 9166586google scholar: lookup
          39. Hameister H, Klett C, Bruch J, Dixkens C, Vogel W, Christensen K. Zoo-FISH analysis: the American mink (Mustela vison) closely resembles the cat karyotype.. Chromosome Res 1997 Feb;5(1):5-11.
            doi: 10.1023/a:1018433200553pubmed: 9088638google scholar: lookup
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