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Blood transfusion = Trasfusione del sangue2022; 21(4); 314-326; doi: 10.2450/2022.0118-22

ZOOMICS : comparative metabolomics of red blood cells from dogs, cows, horses and donkeys during refrigerated storage for up to 42 days.

Abstract: The use of omics technologies in human transfusion medicine has improved our understanding of the red blood cell (RBC) storage lesion(s). Despite significant progress towards understanding the storage lesion(s) of human RBCs, a comparison of basal and post-storage RBC metabolism across multiple species using omics technologies has not yet been reported, and is the focus of this study. Blood was collected in a standard bag system (CPD-SAG-Mannitol) from dogs (n=8), horses, bovines, and donkeys (n=6). All bags were stored at 4°C for up to 42 days (i.e., the end of the shelf life in Italian veterinary clinics) and sampled weekly for metabolomics analyses. In addition, data comparisons to our ongoing Zoomics project are included to compare this study's results with those of non-human primates and humans. Significant interspecies differences in RBC metabolism were observed at baseline, at the time of donation, with bovine showing significantly higher levels of metabolites in the tryptophan/kynurenine pathway; dogs showing elevated levels of high-energy compounds (especially adenosine triphosphate and S-adenosyl-methionine) and equine (donkey and horse) RBCs showing almost overlapping phenotypes, with the highest levels of free branched chain amino acids, glycolytic metabolites (including 2,3-diphosphoglycerate), higher total glutathione pools, and elevated metabolites of the folate pathway compared to the other species. Strikingly, previously described metabolic markers of the storage lesion(s) in humans followed similar trends across all species, though the rate of accumulation/depletion of metabolites in energy and redox metabolism varied by species, with equine blood showing the lowest degree of storage lesion(s). These results interrogate RBC metabolism across a range of mammalian species and improve our understanding of both human and veterinary blood storage and transfusion.
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Publication Date: 2022-07-25 PubMed ID: 35969134PubMed Central: PMC10335344DOI: 10.2450/2022.0118-22Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the differences in red blood cell (RBC) metabolism across various animal species—dogs, cows, horses, and donkeys—when their blood is stored under refrigeration for a period of up to 42 days. The aim is to compare these findings against human and non-human primate data, with the ultimate goal of enhancing understanding about transfusion medicine processes.

Research Methodology

  • Subjects: Blood samples were collected from dogs (8 samples), horses, cows, and donkeys (6 samples each). The rationale for the choice of these animals was not provided in the abstract. The study could have likely chosen these species to represent a broad range of mammalian species.
  • Storage: This study replicated storage conditions typically used in veterinary clinics in Italy. Blood collected was stored in a standard bag system (CPD-SAG-Mannitol) at a temperature of 4°C for up to 42 days. This period matches the typical shelf life of blood under these storage conditions. Thus, the research not only seeks to understand blood storage but also directly relates to pragmatic storage needs.
  • Data Analysis: For the analysis, weekly samples were taken for metabolomics study, looking to understand the changes and differences in RBC metabolism.

Results

  • Baseline differences: Notably, the researchers found significant differences in RBC metabolism at the time of donation across the species samples. Metrics such as metabolite levels in the tryptophan/kynurenine pathway, high-energy compounds, free branched-chain amino acids, glycolytic metabolites, total glutathione pools, and metabolites of the folate pathway showed variations among the species.
  • Change over time: Researchers also noted that metabolic markers associated with storage lesions in humans showed similar trends across all species, albeit at varying rates. Specifically, they observed a difference in the rate of depletion or accumulation of metabolites affecting energy and redox metabolism, with horse blood showing the least deterioration.

Conclusion

From these observations, it can be inferred that the metabolism of RBCs differs significantly across species, and these differences become more pronounced with the extended shelf life of the blood. The understanding gained from this research adds to the field of transfusion medicine, particularly around the storage and utilization of blood products in both human and veterinary contexts. It improves our understanding of the species-specific physiological characteristics of RBCs, which might be helpful in effectively managing and predicting the outcomes of blood transfusion in various species.

Cite This Article

APA
Miglio A, Maslanka M, Di Tommaso M, Rocconi F, Nemkov T, Buehler PW, Antognoni MT, Spitalnik SL, D'Alessandro A. (2022). ZOOMICS : comparative metabolomics of red blood cells from dogs, cows, horses and donkeys during refrigerated storage for up to 42 days. Blood Transfus, 21(4), 314-326. https://doi.org/10.2450/2022.0118-22

Publication

Blood transfusion = Trasfusione del sangue
ISSN: 2385-2070
NlmUniqueID: 101237479
Country: Italy
Language: English
Volume: 21
Issue: 4
Pages: 314-326

Researcher Affiliations

Miglio, Arianna
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Maslanka, Mark
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.
Di Tommaso, Morena
  • Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
Rocconi, Francesca
  • Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
Nemkov, Travis
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.
Buehler, Paul W
  • Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, United States of America.
  • Department of Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland School of Medicine, Baltimore, MD, United States of America.
Antognoni, Maria T
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Spitalnik, Steven L
  • Department of Pathology & Cell Biology, Columbia University, New York, NY, United States of America.
D'Alessandro, Angelo
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.
  • Department of Medicine, Division of Hematology, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.

MeSH Terms

  • Female
  • Horses
  • Humans
  • Animals
  • Cattle
  • Dogs
  • Equidae
  • Blood Preservation / methods
  • Erythrocytes / metabolism
  • Metabolomics / methods
  • Glycolysis

Grant Funding

  • R01 HL146442 / NHLBI NIH HHS
  • R01 HL161004 / NHLBI NIH HHS
  • R01 HL148151 / NHLBI NIH HHS
  • R21 HL150032 / NHLBI NIH HHS
  • R01 HL149714 / NHLBI NIH HHS

Conflict of Interest Statement

. Though unrelated to the contents of this manuscripts, the authors declare that AD and TN are founders of Omix Technologies Inc. AD is also a consultant for Altis Biosciences LLC., Rubius Inc. and Forma Inc. AD and SLS are both consultants for Hemanext Inc. SLS is also a consultant for Tioma, Inc., TCIP, Inc., and the Executive Director of the Worldwide Initiative for Rh Disease Eradication (WIRhE). All the other authors disclose no conflicts of interest relevant to this study.

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Citations

This article has been cited 10 times.
  1. D'Alessandro A, Anastasiadi AT, Tzounakas VL, Nemkov T, Reisz JA, Kriebardis AG, Zimring JC, Spitalnik SL, Busch MP. Red Blood Cell Metabolism In Vivo and In Vitro. Metabolites 2023 Jun 27;13(7).
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