Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.
Publisher:
The Society,. [Cham] : Springer (2022)
Frequency: Monthly, 1999-
Country: Switzerland
Language: English
Author(s):
Nihon Bunseki Kagakkai.
Start Year:1985 -
ISSN:
0910-6340 (Print)
1348-2246 (Electronic)
0910-6340 (Linking)
1348-2246 (Electronic)
0910-6340 (Linking)
Impact Factor
1.6
2022
| NLM ID: | 8511078 |
| (DNLM): | SR0054047(s) |
| (OCoLC): | 12754332 |
| Coden: | ANSCEN |
| LCCN: | sn 86031135 |
| Classification: | W1 AN1918T |
Simultaneous doping analysis of main urinary metabolites of anabolic steroids in horse by ion-trap gas chromatography-tandem mass spectrometry. The use of anabolic steroids in racehorses is strictly regulated. We have developed a method for the simultaneous analysis of 11 anabolic steroids: fluoxymesterone, 17alpha-methyltestosterone, mestanolone, methandienone, methandriol, oxymetholone, boldenone, furazabol, methenolone, nandrolone, and stanozolol, for possible application to a doping test in racehorses. We selected 15 kinds of target substances for a doping test from the main metabolites of these anabolic steroids, and established a method for simultaneous analysis. Urine was hydrolyzed and subjected to solid-phase extraction. Then...
Characterization and quantification of fluoxymesterone metabolite in horse urine by gas chromatography/mass spectrometry. Fluoxymesterone, an anabolic steroid with the 17alpha-methyl,17beta-hydroxy group, has been developed as an oral formulation for therapeutic purposes. However, it is also used illegally in racehorses to enhance racing performance. In this study, we detected 9alpha-fluoro-17,17-dimethyl-18-norandrostane-4,13-dien-11beta-ol-3-one by gas chromatography/mass spectrometry (GC/MS), which has not been reported as a fluoxymesterone metabolite so far in horse. It was synthesized for use as a reference standard, and characterized on the basis of (1)H NMR and (13)C NMR spectra, as well as GC/MS EI mass s...