Biochemical and biophysical research communications.

Characterization and differentiation of equine umbilical cord-derived matrix cells.

Biochemical and biophysical research communications August 13, 2007 Volume 362, Issue 2 347-353 doi: 10.1016/j.bbrc.2007.07.182

Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L, Sand T, Mitchell KE.Stem cells are being evaluated in numerous human clinical trials and are commercially used in veterinary medicine to treat horses and dogs. Stem cell differentiation, homing to disease sites, growth and cytokine factor modulation, and low antigenicity contribute to their therapeutic success. Bone marrow and adipose tissue are the two most common sources of adult-derived stem cells in animals. We report on the existence of an alternative source of primitive, multipotent stem cells from the equine umbilical cord cellular matrix (Wharton's jelly). Equine umbilical cord matrix (EUCM) cells can be ...

Biochemical and biophysical research communications April 28, 1989 Volume 160, Issue 2 486-494 doi: 10.1016/0006-291x(89)92459-5

Roberts MM, Oroszlan S.Capsids of equine infectious anemia virus have been isolated as cone-shaped particles 60 x 120 nm in size. Detergent treatment of whole virus followed by two cycles of rate-zonal centrifugation in Ficoll produces these capsids in a yield of approximately 10%. The major protein components are the gag-encoded p11 nucleocapsid protein and p26 capsid protein, which are present in equimolar amounts. Substantial cleavage of p11 to p6 and p4 can be observed under conditions where the viral protease packaged in the capsid is enzymatically active.

Biochemical and biophysical research communications July 29, 2000 Volume 274, Issue 2 467-471 doi: 10.1006/bbrc.2000.3167

Iqbal J, Dudhia J, Bird JL, Bayliss MT.The synthesis of proteoglycans was measured in normal equine articular cartilage of ages 9 months to 20 years and the effect of TGF-beta1 on this activity was investigated. The rate of incorporation of [(35)S]Na(2)SO(4) decreased with age as did the responsiveness of the tissue to the growth factor. The enhanced synthesis of proteoglycan induced at all ages by TGF-beta1 was down-regulated by IL-1 beta and retinoic acid. The expression of mRNA for TGF-beta1, 2, and 3 was also measured, and although the level of TGF-beta1 was highest at all ages, the expression of each growth factor decreased wi...

Biochemical and biophysical research communications December 23, 1974 Volume 61, Issue 4 1400-1406 doi: 10.1016/s0006-291x(74)80439-0

Corradin G, Harbury HA.No abstract available

Biochemical and biophysical research communications November 24, 2010 Volume 404, Issue 1 190-194 doi: 10.1016/j.bbrc.2010.11.091

Ascenzi P, Ciaccio C, Sinibaldi F, Santucci R, Coletta M.Upon interaction with bovine heart cardiolipin (CL), horse heart cytochrome c (cytc) changes its tertiary structure disrupting the heme-Fe-Met80 distal bond, reduces drastically the midpoint potential out of the range required for its physiological role, binds CO and NO with high affinity, and displays peroxidase activity. Here, the effect of CL on peroxynitrite isomerization by ferric cytc (cytc-Fe(III)) is reported. In the absence of CL, hexa-coordinated cytc does not catalyze peroxynitrite isomerization. In contrast, CL facilitates cytc-Fe(III)-mediated isomerization of peroxynitrite in a d...

Biochemical and biophysical research communications June 12, 2006 Volume 346, Issue 3 1108-1113 doi: 10.1016/j.bbrc.2006.06.022

Rodríguez-Roldán V, García-Heredia JM, Navarro JA, Hervás M, De la Cerda B, Molina-Heredia FP, De la Rosa MA.Two synthetic genes coding for human and Arabidopsis cytochrome c, respectively, have been designed and constructed, and the recombinant proteins have been over-expressed in Escherichia coli cells. Thus a comparative analysis of the two heme proteins, including horse cytochrome c as a reference, has been performed. In addition to their physico-chemical properties, the redox behavior of the three proteins has been analyzed by following the kinetics of both their reduction by flavin semiquinones (lumiflavin, riboflavin, and FMN) and oxidation by cytochrome c oxidase. The resulting data indicate ...

Biochemical and biophysical research communications October 16, 2004 Volume 324, Issue 3 1108-1115 doi: 10.1016/j.bbrc.2004.09.154

Ghim SJ, Rector A, Delius H, Sundberg JP, Jenson AB, Van Ranst M.Equus caballus papillomavirus type 1 (EcPV-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. The complete EcPV-1 nucleotide sequence and genomic organization were determined. Phylogenetic analysis showed that EcPV-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. To produce EcPV-1 virus-like particles (VLPs), the EcPV-1 L1 major capsid protein was expressed in insect cells using a recombinant baculovirus vector. The self-assembled EcPV-1 VLPs were morphologically indistingui...

Biochemical and biophysical research communications October 14, 1964 Volume 17, Issue 3 211-214 doi: 10.1016/0006-291x(64)90385-7

Akeson A.No abstract available

Biochemical and biophysical research communications August 8, 1977 Volume 77, Issue 3 1024-1029 doi: 10.1016/s0006-291x(77)80080-6

Hudgins PM, Bond GH.No abstract available

Biochemical and biophysical research communications January 29, 2010 Volume 393, Issue 2 196-200 doi: 10.1016/j.bbrc.2010.01.092

Ascenzi P, di Masi A, Gullotta F, Mattu M, Ciaccio C, Coletta M.Cyanide binds to ferric heme-proteins with a very high affinity, reflecting the very low dissociation rate constant (k(off)). Since no techniques are available to estimate k(off), we report herewith a method to determine k(off) based on the irreversible reductive nitrosylation reaction to trap ferric myoglobin (Mb(III)). The k(off) value for cyanide dissociation from ferric cyanide horse heart myoglobin (Mb(III)-cyanide) was determined at pH 9.2 and 20.0 degrees C. Mixing Mb(III)-cyanide and NO solutions brings about absorption spectral changes reflecting the disappearance of Mb(III)-cyanide w...

Biochemical and biophysical research communications November 30, 1981 Volume 103, Issue 2 573-580 doi: 10.1016/0006-291x(81)90490-3

Nishita T, Deutsch HF.No abstract available

Biochemical and biophysical research communications May 20, 1974 Volume 58, Issue 2 446-453 doi: 10.1016/0006-291x(74)90385-4

Boeynaems JM, Pochet R, Dumont JE.No abstract available

Biochemical and biophysical research communications September 23, 1997 Volume 238, Issue 1 81-85 doi: 10.1006/bbrc.1997.7246

Bird JL, Wells T, Platt D, Bayliss MT.Proteoglycan degradation was induced in young equine articular cartilage explants cultured for eight days in the presence of 50 ng/ml recombinant human interleukin-1 beta. Degradation was initiated after 6 hours of exposure to the cytokine. This was accompanied by an induction of nitric oxide synthesis and a decrease in the incorporation of [36S]sulphate into the glycosaminoglycan chains of proteoglycans. The addition of 1mM N-iminoethyl-L-ornithine (an inhibitor of nitric oxide synthase) to the explant cultures in the presence of rhIL-1 beta suppressed the synthesis of NO and restored proteog...

Biochemical and biophysical research communications February 10, 2013 Volume 432, Issue 2 256-261 doi: 10.1016/j.bbrc.2013.02.002

Bonin CP, Baccarin RY, Nostell K, Nahum LA, Fossum C, de Camargo MM.Engagement of Toll-like receptor 4 (TLR4) by lipopolysaccharide (LPS) is a master trigger of the deleterious effects of septic shock. Horses and humans are considered the most sensitive species to septic shock, but the mechanisms explaining these phenomena remain elusive. Analysis of tlr4 promoters revealed high similarity among LPS-sensitive species (human, chimpanzee, and horse) and low similarity with LPS-resistant species (mouse and rat). Four conserved nuclear factor kappa B (NFκB) binding sites were found in the tlr4 promoter and two in the md2 promoter sequences that are likely to be t...

Biochemical and biophysical research communications March 29, 2006 Volume 343, Issue 3 928-936 doi: 10.1016/j.bbrc.2006.03.055

Podolsky MJ, Lasker A, Flaminio MJ, Gowda LD, Ezekowitz RA, Takahashi K.The mannose-binding lectin (MBL), a pattern recognition serum protein, participates in the innate immune system of mammals as an opsonin. In humans, MBL plays a key role in first-line host defense against infection during the lag period prior to the development of a specific immune response. MBL also activates complement via the lectin pathway that requires a MBL-associated serine protease-2 (MASP-2). Homologues of human MBL (hMBL) have been identified in a variety of mammals, fish, and primitive animals such as ascidians. In this study, we report that equine MBL (eMBL) has properties that are...

Biochemical and biophysical research communications January 31, 1991 Volume 174, Issue 2 940-945 doi: 10.1016/0006-291x(91)91509-b

Matsui T, Sugino H, Miura M, Bousfield GR, Ward DN, Titani K, Mizuochi T.The glycoprotein hormones, equine chorionic gonadotropin (eCG) and lutenizing hormone (eLH), possess a beta-subunit with an identical amino acid sequence. The Asn-linked oligosaccharide chains of eCG beta and eLH beta were quantitatively liberated as tritium-labeled oligosaccharides by hydrazinolysis followed by N-acetylation and NaB3H4-reduction. Paper electrophoresis in combination with sialidase digestion and solvolytic desulfation indicated that eCG beta contained neutral and sialylated oligosaccharides, while eLH beta contained neutral, sialylated, sulfated, and both sialylated and sulfat...

Biochemical and biophysical research communications May 20, 1974 Volume 58, Issue 2 397-404 doi: 10.1016/0006-291x(74)90378-7

Papkoff H.No abstract available

Biochemical and biophysical research communications October 29, 1979 Volume 90, Issue 4 1387-1392 doi: 10.1016/0006-291x(79)91189-6

Poiree JC, Mengual R, Sudaka P.No abstract available

Biochemical and biophysical research communications December 15, 1975 Volume 67, Issue 4 1488-1493 doi: 10.1016/0006-291x(75)90194-1

Sytkowski AJ, Vallee BL.The catalytic and non-catalytic Zn atom pairs of horse liver alcohol dehydrogenase (LADH) have been replaced sequentially either by 65Zn, Co or 65Zn and Co. The Co derivatives exhibit characteristic spectra. When Co replaces the Zn atoms which exchange secondly, enzymatic activity is altered, and both imidazole and 1,10-phenanthroline (OP) significantly modify the spectrum of the catalytic Co atoms. Further, due to the removal of cobalt, the instantaneous and reversible OP inhibition of the native enzyme becomes time-dependent and irreversible. Jointly, these data identify the pair of metal at...

Biochemical and biophysical research communications March 3, 1975 Volume 63, Issue 1 43-49 doi: 10.1016/s0006-291x(75)80008-8

Seybert DW, Moffat K, Gibson QH.No abstract available

Biochemical and biophysical research communications May 30, 1994 Volume 201, Issue 1 201-207 doi: 10.1006/bbrc.1994.1689

Robert P, Amsellem S, Christophe S, Benifla JL, Bellet D, Koman A, Bidart JM.To investigate the possibility that specific structural determinants within the equine follitropin receptor (eFSHR) are critical to the enhanced specificity of this receptor compared to other FSHRs, we used the RACE-PCR technique to clone the eFSHR from equine testis. Sequence analysis revealed that the eFSHR is highly homologous to other mammal FSHRs, but it presents 10 unique amino acid residue replacements in the extracellular domain. Furthermore, a potential N-glycosylation site was detected at a position not encountered in other receptors. Northern blot analysis identified three transcrip...

Biochemical and biophysical research communications March 11, 2015 Volume 459, Issue 4 668-672 doi: 10.1016/j.bbrc.2015.02.168

Michalek M, Jung S, Shomali MR, Cauchard S, Sönnichsen FD, Grötzinger J.Defensins are small effector molecules of the innate immune system that are present in almost all organisms including plants and animals. These peptides possess antimicrobial activity against a broad range of microbes including bacteria, fungi and viruses and act as endogenous antibiotics. α-Defensins are a subfamily of the defensin family and their expression is limited to specific tissues. Equine DEFA1 is an enteric α-defensin exclusively secreted by Paneth cells and shows an activity against a broad spectrum of microbes, including typical pathogens of the horse such as Rhodococcus equi, v...

Biochemical and biophysical research communications November 14, 1978 Volume 85, Issue 1 7-13 doi: 10.1016/s0006-291x(78)80003-5

Pande A, Myer YP.No abstract available

Biochemical and biophysical research communications April 5, 2016 Volume 473, Issue 4 1058-1063 doi: 10.1016/j.bbrc.2016.04.015

Kumar CS, Swamy MJ.The major protein of equine seminal plasma, HSP-1/2 exhibits chaperone-like activity and protects a variety of target proteins against thermal and chemical stress conditions. Here, we show that HSP-1/2 is able to protect enzymes such as alcohol dehydrogenase and glucose-6-phosphate dehydrogenase against H2O2 induced stress, clearly demonstrating that HSP-1/2 acts as a chaperone against oxidative stress. Further, the present studies show that HSP-1/2 also inhibits lipid (linoleic acid) peroxidation by hydroxyl radicals in vitro. These results are of great significance considering that so far l...

Biochemical and biophysical research communications November 14, 1991 Volume 180, Issue 3 1365-1371 doi: 10.1016/s0006-291x(05)81346-4

Thomas DA, Furman PA.The reverse transcriptase of Equine Infectious Anemia Virus (EIAV) was partially purified from virus particles and appeared to be a heterodimer with subunit molecular masses of 70 kdal and 59 kdal. The polymerase activity of this enzyme had an absolute requirement for a divalent cation, preferring Mg++ over Mn++. Addition of a monovalent cation to the reaction mixture enhanced, but was not required for enzyme activity. Kinetically, the reverse transcriptase of EIAV is similar to the reverse transcriptase of Human Imunodeficiency Virus Type 1 (HIV-1). Both enzymes have similar Km values for 2'-...

Biochemical and biophysical research communications December 17, 1971 Volume 45, Issue 6 1444-1450 doi: 10.1016/0006-291x(71)90182-3

Ward RL, Happe JA.No abstract available

Biochemical and biophysical research communications July 13, 2000 Volume 273, Issue 3 816-819 doi: 10.1006/bbrc.2000.3021

Myshkin AE, Khromova VS.Histidin has been shown to effectively inhibit coagulation of horse oxyhemoglobin (HbO(2)) modified by mercury(II) ion bound to reactive thiol groups of protein. Kinetic parameters were measured and the histidin-to-mercury binding constant was kinetically estimated. Histidin, as other pharmaceutically acceptable compounds with some mercury-binding capacity, has been suggested to alleviate mercury intoxication conditions.

Biochemical and biophysical research communications December 30, 1992 Volume 189, Issue 3 1255-1260 doi: 10.1016/0006-291x(92)90208-3

Prosser CG, McLaren RD.Ligand blotting analysis of serum from the horse using radiolabelled IGF-I revealed a protein at 96 kDa which was not present in serum from goat, cow, sheep, deer or donkey. These latter species all displayed five labelled bands in the range 24 to 41 kDa. Conversely, these were only weakly labelled in serum from the horse. Size exclusion chromatography of horse serum pre-incubated with radiolabelled IGF-I revealed reduced binding in the 130-kDa peak compared with goat plasma, and ligand blotting analysis indicated the 96-kDa protein was present in this peak. The 96-kDa protein from horse serum...

Biochemical and biophysical research communications July 30, 1982 Volume 107, Issue 2 435-441 doi: 10.1016/0006-291x(82)91510-8

Dianoux AC, Freyssinet JM.No abstract available

Biochemical and biophysical research communications November 25, 2003 Volume 312, Issue 1 91-96 doi: 10.1016/j.bbrc.2003.09.215

Toscano WA, Toscano JS, Toscano DG, Gross MK.The soluble calmodulin-sensitive isoform of adenylyl cyclase isolated from equine sperm is unique because it requires Mn(2+) rather than Mg(2+) for activity. To gain insight into the molecular action of metals on sperm adenylyl cyclase, the kinetics of Mn(2+) and ATP effect was examined. A biphasic response to increases in ATP concentration was observed when metal was held constant. When [Mn(2+)] exceeded [ATP], however, greatly enhanced enzyme activity was observed. The kinetic profiles were consistent with allosteric activation of adenylyl cyclase by Mn(2+). Linear transformation of the data...

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NLM ID:	0372516
(DNLM):	B14760000(s)
(OCoLC):	01532958
Coden:	BBRCA9
Classification:	W1 BI6198

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